TRIFLORINE 10S: A BIO-BASED FORMULATION FOR MANAGING EARLY BLIGHT OF TOMATO

Abstract

This invention provides a bio-based formulation, Triflorine 10S, for controlling early blight in tomato plants. Comprising extracts from Desmodium triflorum, ginger, sacred basil, and vermicompost, the formulation offers a sustainable alternative to synthetic fungicides. Triflorine 10S enhances plant resilience, reduces pathogen resistance, and supports eco-friendly agricultural practices by providing prolonged protection against A. solani.

Specification

Description:FIELD OF THE INVENTION
This invention relates to agricultural biotechnology, particularly a bio-based formulation designed to control early blight in tomato plants caused by Alternaria solani. The formulation leverages natural bio-compounds, providing a sustainable alternative to synthetic fungicides, enhancing crop resilience and promoting eco-friendly agricultural practices.
BACKGROUND OF THE INVENTION
Early blight, primarily caused by the fungal pathogen Alternaria solani, is a severe disease that affects tomato crops, leading to reduced yield and compromised fruit quality. Conventional approaches to managing early blight often rely on synthetic fungicides, which can have adverse effects on the environment, human health, and beneficial organisms. The excessive use of these chemicals has led to increased pathogen resistance, necessitating the development of alternative, sustainable solutions.
While bio-based fungicides exist, there is a gap in formulations specifically targeting early blight in tomatoes with proven efficacy and safety. The invention of Triflorine 10S introduces a novel combination of bioactive compounds derived from Desmodium triflorum, ginger, sacred basil, and vermicompost, which collectively inhibit the growth of A. solani and enhance plant health. The unique delivery system in Triflorine 10S ensures prolonged activity on plant surfaces, addressing challenges faced by existing bio-based treatments.
Triflorine 10S not only targets early blight but also contributes to the overall health of tomato plants by boosting natural defenses and reducing reliance on synthetic fungicides. This invention provides an effective and sustainable approach to disease management, supporting healthier crops and eco-friendly farming practices.
SUMMARY OF THE INVENTION
This summary is provided to introduce a selection of concepts, in a simplified format, that are further described in the detailed description of the invention.
This summary is neither intended to identify key or essential inventive concepts of the invention and nor is it intended for determining the scope of the invention.
To further clarify advantages and features of the present invention, a more particular description of the invention will be rendered by reference to specific embodiments thereof, which is illustrated in the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail with the accompanying drawings.
The invention provides a bio-based formulation, Triflorine 10S, that utilizes bio-compounds from Desmodium triflorum, ginger, sacred basil, and vermicompost to control early blight in tomato plants. The formulation combines active ingredients that inhibit fungal growth and enhance plant resilience, offering a safe and effective alternative to synthetic fungicides. Triflorine 10S is designed for prolonged efficacy, adhering to plant surfaces to provide lasting protection against A. solani while promoting sustainable agriculture.
DETAILED DESCRIPTION OF THE INVENTION
The detailed description of various exemplary embodiments of the disclosure is described herein with reference to the accompanying drawings. It should be noted that the embodiments are described herein in such details as to clearly communicate the disclosure. However, the amount of details provided herein is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the present disclosure as defined by the appended claims.
It is also to be understood that various arrangements may be devised that, although not explicitly described or shown herein, embody the principles of the present disclosure. Moreover, all statements herein reciting principles, aspects, and embodiments of the present disclosure, as well as specific examples, are intended to encompass equivalents thereof.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms "a"," "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises," "comprising," "includes" and/or "including," when used herein, specify the presence of stated features, integers, steps, operations, elements and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and/or groups thereof.
It should also be noted that in some alternative implementations, the functions/acts noted may occur out of the order noted in the figures. For example, two figures shown in succession may, in fact, be executed concurrently or may sometimes be executed in the reverse order, depending upon the functionality/acts involved.
In addition, the descriptions of "first", "second", "third", and the like in the present invention are used for the purpose of description only, and are not to be construed as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may include at least one of the features, either explicitly or implicitly.
Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, e.g., those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The Triflorine 10S formulation process begins with the extraction of bioactive compounds from the leaves of Desmodium triflorum, fresh ginger rhizomes, and sacred basil leaves. Each plant material is washed thoroughly, shade-dried to prevent degradation of bioactive components, and ground into a fine powder. The powdered D. triflorum leaves are soaked in 95% ethanol for 24 hours to extract active compounds, filtered, and evaporated to yield the extract. Fresh ginger and sacred basil are blended with distilled water to obtain their respective juices, which are filtered to remove solids.
The extracted bio-compounds are then combined with vermicompost in a 1:10 ratio (w/v), providing a substrate rich in organic matter and microbial activity that enhances the efficacy of the formulation. The addition of a natural emulsifier, such as lecithin, stabilizes the formulation, while pH adjustments using citric acid or sodium bicarbonate maintain the pH at approximately 7.0 for optimal application.
Characterization of the Formulation is performed using high-performance liquid chromatography (HPLC) to analyze the presence and concentration of active bio-compounds. Antioxidant and antimicrobial activities are documented through DPPH and agar diffusion methods, respectively, establishing the formulation's efficacy in inhibiting A. solani.
Efficacy Testing involves both laboratory and pot trials. In laboratory tests, the poisoned food technique is used, where varying concentrations of the extract are applied to potato dextrose agar (PDA) plates inoculated with A. solani, assessing fungal growth inhibition. Pot trials are conducted by applying the formulation in the soil and spraying it on tomato plants infected with early blight, observing disease progression, plant growth, and yield over a four-week period.
, Claims:1. A bio-based formulation for managing early blight in tomato plants, comprising bio-compounds from Desmodium triflorum, ginger, sacred basil, and vermicompost.
2. The formulation as claimed in Claim 1, wherein Desmodium triflorum is extracted with 95% ethanol to yield bioactive compounds.
3. The formulation as claimed in Claim 1, wherein ginger and sacred basil are blended with distilled water for juice extraction.
4. The formulation as claimed in Claim 1, wherein vermicompost serves as a substrate, providing microbial activity that enhances bio-compound efficacy.
5. The formulation as claimed in Claim 1, wherein a natural emulsifier is added to stabilize the formulation.
6. The formulation as claimed in Claim 1, wherein the pH is adjusted to approximately 7 using citric acid or sodium bicarbonate.
7. A method of application as claimed in Claim 1, involving the foliar spray and soil application for disease management in tomato plants.
8. The formulation as claimed in Claim 1, wherein high-performance liquid chromatography (HPLC) characterizes bio-compound concentration and efficacy.
9. The method as claimed in Claim 1, wherein the formulation inhibits A. solani growth through antioxidant and antimicrobial bio-compounds.

10. The formulation as claimed in Claim 1, wherein it reduces reliance on synthetic fungicides and promotes eco-friendly tomato cultivation

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