NYCTANTHES ARBOR-TRISTIS-DERIVED CARBON DOTS FOR BIO-IMAGING APPLICATIONS

Abstract

ABSTRACT “NYCTANTHES ARBOR-TRISTIS-DERIVED CARBON DOTS FOR BIO-IMAGING APPLICATIONS” The present invention relates to the green synthesis of nitrogen-doped fluorescent carbon dots derived from Nyctanthes arbor-tristis leaf extract, a novel and sustainable precursor. The synthesized carbon dots exhibit excellent water solubility, low cytotoxicity, and significant photoluminescence properties, making them ideal for bioimaging applications, including cancer cell imaging. These carbon dots are specifically designed to image MCF-7 cancer cells with high accuracy. The synthesis process is environmentally friendly, requiring low energy and minimizing environmental impact. The carbon dots demonstrate high photostability, brightness, and contrast, offering superior imaging performance in biomedical diagnostics. The invention provides a sustainable, cost-effective alternative to conventional imaging agents and is applicable in various medical and research fields, including drug delivery systems and diagnostic kits. Figure 1

Specification

Description:TECHNICAL FIELD
[0001] The present invention relates to the field of bio-imagining, and more particularly, the present invention relates to the Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications.
BACKGROUND ART
[0002] The following discussion of the background of the invention is intended to facilitate an understanding of the present invention. However, it should be appreciated that the discussion is not an acknowledgment or admission that any of the material referred to was published, known, or part of the common general knowledge in any jurisdiction as of the application's priority date. The details provided herein the background if belongs to any publication is taken only as a reference for describing the problems, in general terminologies or principles or both of science and technology in the associated prior art.
[0003] Bioimaging is a non-invasive technique used to visualize biological processes over time without disrupting normal functions like movement or respiration. This method facilitates the visualization of three-dimensional structures within specimens and allows for detailed observation of both subcellular components and tissues within multicellular organisms. It employs a variety of sources for imaging, including light, fluorescence, electrons, ultrasound, X-rays, magnetic resonance, and positrons.
[0004] Recent advances in nanotechnology have led to the development of various nanomaterials for bioimaging. Carbon dots have gained prominence due to their low toxicity, chemical stability, and exceptional photoluminescence properties. While synthetic routes for carbon nanodots typically involve complex and costly chemical processes there is a growing interest in sourcing carbon nanodots from materials to reduce production costs and environmental impact.
[0005] Nyctanthes arbor-tristis, a plant commonly used in traditional medicine, presents a largely untapped source of raw material rich in carbon and other essential elements conducive to forming high-quality carbon nanodots.
[0006] While MRI, CT, ultrasound, SPECT, and PET are typically employed for lesion detection, their imaging resolution is generally macroscopic. In contrast, fluorescence imaging technology offers the highest spatial resolution for diagnosing diseases at a microscopic level.
[0007] In light of the foregoing, there is a need for Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications that overcomes problems prevalent in the prior art associated with the traditionally available method or system, of the above-mentioned inventions that can be used with the presented disclosed technique with or without modification.
[0008] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies, and the definition of that term in the reference does not apply.
OBJECTS OF THE INVENTION
[0009] The principal object of the present invention is to overcome the disadvantages of the prior art by providing Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications.
[0010] Another object of the present invention is to provide Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications that can be used in cancer cell bioimaging applications.
[0011] Another object of the present invention is to provide Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications that can be used in different biomedical applications.
[0012] Another object of the present invention is to provide Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications that is environmentally benign, utilizing cost-effective and energy-efficient materials without generating toxic byproducts. It employs plant-based materials, specifically leaf extract from Nyctanthes arbor-tristis, which is non-toxic to the environment.
[0013] The foregoing and other objects of the present invention will become readily apparent upon further review of the following detailed description of the embodiments as illustrated in the accompanying drawings.
SUMMARY OF THE INVENTION
[0014] The present invention relates to Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications.
[0015] Green synthesis of novel nitrogen-doped fluorescent carbon dots from an unreported Nyctanthes arbor-tristis leaf extract as a green precursor for the cancer cell imaging.
[0016] They are water-soluble and have low cytotoxicity and excellent photoluminescence properties.
[0017] This formulation shows significant fluorescence imaging of cancer cells.
[0018] Here we used MCF-7 cancer cell line for the bioimaging applications.
[0019] The novel carbon dots are synthesized using the leaf extract of Nyctanthes arbor-tristis, a method not previously documented for the synthesis of carbon dots. This use of a novel plant-based precursor introduces unique phytochemical properties to the carbon dots, potentially enhancing their biocompatibility and imaging capabilities. The Nyctanthes arbor-tristis-derived carbon dot has never been used in cancer cell imaging applications. The synthesized carbon dots exhibit excellent photoluminescence properties.
[0020] While the invention has been described and shown with reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.
BRIEF DESCRIPTION OF DRAWINGS
[0021] So that the manner in which the above-recited features of the present invention can be understood in detail, a more particular description of the invention, briefly summarized above, may have been referred by embodiments, some of which are illustrated in the appended drawings. It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered limiting of its scope, for the invention may admit to other equally effective embodiments.
[0022] These and other features, benefits, and advantages of the present invention will become apparent by reference to the following text figure, with like reference numbers referring to like structures across the views, wherein:
[0023] Figure 1 shows a schematic representation of synthesis and bioimaging application of nitrogen-doped carbon dots.
DETAILED DESCRIPTION OF THE INVENTION
[0024] While the present invention is described herein by way of example using embodiments and illustrative drawings, those skilled in the art will recognize that the invention is not limited to the embodiments of drawing or drawings described and are not intended to represent the scale of the various components. Further, some components that may form a part of the invention may not be illustrated in certain figures, for ease of illustration, and such omissions do not limit the embodiments outlined in any way. It should be understood that the drawings and the detailed description thereto are not intended to limit the invention to the particular form disclosed, but on the contrary, the invention is to cover all modifications, equivalents, and alternatives falling within the scope of the present invention as defined by the appended claim.
[0025] As used throughout this description, the word "may" is used in a permissive sense (i.e. meaning having the potential to), rather than the mandatory sense, (i.e. meaning must). Further, the words "a" or "an" mean "at least one" and the word "plurality" means "one or more" unless otherwise mentioned. Furthermore, the terminology and phraseology used herein are solely used for descriptive purposes and should not be construed as limiting in scope. Language such as "including," "comprising," "having," "containing," or "involving," and variations thereof, is intended to be broad and encompass the subject matter listed thereafter, equivalents, and additional subject matter not recited, and is not intended to exclude other additives, components, integers, or steps. Likewise, the term "comprising" is considered synonymous with the terms "including" or "containing" for applicable legal purposes. Any discussion of documents, acts, materials, devices, articles, and the like are included in the specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention.
[0026] In this disclosure, whenever a composition or an element or a group of elements is preceded with the transitional phrase "comprising", it is understood that we also contemplate the same composition, element, or group of elements with transitional phrases "consisting of", "consisting", "selected from the group of consisting of, "including", or "is" preceding the recitation of the composition, element or group of elements and vice versa.
[0027] The present invention is described hereinafter by various embodiments with reference to the accompanying drawing, wherein reference numerals used in the accompanying drawing correspond to the like elements throughout the description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiment set forth herein. Rather, the embodiment is provided so that this disclosure will be thorough and complete and will fully convey the scope of the invention to those skilled in the art. In the following detailed description, numeric values and ranges are provided for various aspects of the implementations described. These values and ranges are to be treated as examples only and are not intended to limit the scope of the claims. In addition, several materials are identified as suitable for various facets of the implementations. These materials are to be treated as exemplary and are not intended to limit the scope of the invention.
[0028] The present invention relates to Nyctanthes arbor-tristis-derived carbon dots for bio-imaging applications.
- Green synthesis of novel nitrogen-doped fluorescent carbon dots from an unreported Nyctanthes arbor-tristis leaf extract as a green precursor for the cancer cell imaging.
- They are water-soluble and have low cytotoxicity and excellent photoluminescence properties.
- This formulation shows significant fluorescence imaging of cancer cells.
- Here we used MCF-7 cancer cell line for the bioimaging applications.
[0029] The novel carbon dots are synthesized using the leaf extract of Nyctanthes arbor-tristis, a method not previously documented for the synthesis of carbon dots. This use of a novel plant-based precursor introduces unique phytochemical properties to the carbon dots, potentially enhancing their biocompatibility and imaging capabilities. The Nyctanthes arbor-tristis-derived carbon dot has never been used in cancer cell imaging applications. The synthesized carbon dots exhibit excellent photoluminescence properties.
[0030] This invention provides a new, efficient, and environmentally friendly approach to bioimaging, offering significant improvements over existing materials in terms of safety, stability, and functionality. This innovative use of natural resources to create high-performance imaging agents stands to make a substantial impact in the field of medical diagnostics and research.
[0031] Comparison of advantages between our formulation of carbon nanodots and conventional nanodots and other green synthesized material.
Featured Nyctanthes arbor-tristis-derived carbon dots Conventional carbon dots Other green synthesized carbon dots
Synthesis material Utilized Nyctanthes arbor-tristis leaf extract, a novel and sustainable source.
Cost-effective Often derived from non-renewable resources or less sustainable organic and inorganic materials.
Often more expensive Typically used plant or flower or food waste, but may not always be optimally sustainable
Cost-effective, though dependent on the economic availability of precursor materials.
Toxicity Non-toxic and ideal for biomedical applications Some may contain toxic precursors or residues affecting biocompatibility. Generally low toxicity, but dependent on the precursor materials and synthesis method.
Energy consumption Lower energy is required during synthesis Higher energy is required during synthesis Generally, energy-efficient but varies with the method.
Biocompatibility Excellent biocompatibility Effect of cytotoxicity Good biocompatibility
Though it can vary based on synthesis and purification.
Imaging Exhibits good imaging with better resolution Generally good, but can vary widely based on the surface passivation Good, but the imaging quality based on the synthesis method
[0032] Our developed carbon dot has significant economic potential in the field of biomedical applications because it exhibits good biocompatibility and low toxicity which make it an excellent candidate.
[0033] This synthesized dot can be commercially used in medical imaging, drug delivery systems, and diagnostic kits and can be useful in research and development.
[0034] The utilization of Nyctanthes arbor-tristis leaf extract as a precursor material for synthesizing carbon dots is novel and unprecedented in bioimaging applications.
[0035] The synthesis process is eco-friendly and cost-effective and utilizes plant-based materials which reduces the environmental impact associated with traditional synthesis methods. This feature addresses growing concerns regarding sustainability and environmental impact in material synthesis.
[0036] The synthesis process requires low energy, contributing to its sustainability and eco-friendliness. This feature distinguishes the Nyctanthes arbor-tristis-derived carbon dots from conventional carbon dot synthesis methods which often involve energy-intensive processes.
[0037] The carbon dots derived from Nyctanthes arbor-tristis leaf extract exhibit non-toxic properties, making them highly biocompatible and ensuring less adverse effects on biomedical applications such as bioimaging.
[0038] The developed carbon nanodots exhibit excellent imaging performance, including high photostability, brightness, and contrast. These characteristics contribute to their effectiveness as imaging agents, enabling clear and precise visualization of biological structures and processes in diverse imaging modalities.
[0039] By incorporating these innovative features, the Nyctanthes arbor-tristis-derived carbon dots offer a sophisticated solution for bioimaging applications, addressing key challenges in the field while paving the way for advanced imaging technologies with improved performance and sustainability.
[0040] Various modifications to these embodiments are apparent to those skilled in the art from the description and the accompanying drawings. The principles associated with the various embodiments described herein may be applied to other embodiments. Therefore, the description is not intended to be limited to the 5 embodiments shown along with the accompanying drawings but is to be providing the broadest scope consistent with the principles and the novel and inventive features disclosed or suggested herein. Accordingly, the invention is anticipated to hold on to all other such alternatives, modifications, and variations that fall within the scope of the present invention and appended claims. , Claims:CLAIMS
We Claim:
1) A method for synthesizing nitrogen-doped fluorescent carbon dots, wherein the method comprises the steps of:
- Utilizing Nyctanthes arbor-tristis leaf extract as a green precursor,
- Performing green synthesis to produce water-soluble carbon dots with low cytotoxicity and excellent photoluminescence properties,
- Wherein the synthesized carbon dots exhibit enhanced biocompatibility and are specifically designed for bioimaging applications, including imaging of cancer cells.
2) The carbon dots synthesized by the method of claim 1, wherein the carbon dots are non-toxic and exhibit significant fluorescence imaging capabilities, particularly for MCF-7 cancer cell line imaging.
3) A bioimaging agent comprising nitrogen-doped fluorescent carbon dots synthesized from Nyctanthes arbor-tristis leaf extract, wherein the carbon dots demonstrate high photostability, brightness, and contrast for enhanced imaging performance in biomedical applications.
4) The carbon dots as claimed in claim 3, wherein the carbon dots are synthesized through a low-energy, eco-friendly process, providing a sustainable alternative to conventional carbon dot synthesis methods and reducing the environmental impact.
5) A bio-imaging method utilizing the carbon dots as claimed in claim 3, wherein the method comprises:
- administering the carbon dots to biological tissues or cells, and
- utilizing the photoluminescence properties of the carbon dots to visualize and image biological structures, with the application focused on cancer cell imaging.

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