NUTRIENT-RICH FORMULATION FOR ENHANCING FIBER QUALITY AND YIELD IN COTTON CROPS

Abstract

ABSTRACT The invention presents a novel nanoformulation designed to protect honey bees from the detrimental effects of microplastic contamination. Utilizing advanced nano-encapsulation techniques, this formulation comprises biodegradable nanoparticles that encapsulate a synergistic blend of bioactive agents, including antioxidants, detoxifying enzymes, and chelating agents, ensuring targeted and controlled release. This approach specifically addresses the adverse interactions between microplastics and honey bee physiology, mitigating oxidative stress and digestive blockages caused by microplastic ingestion. The formulation is designed for efficient delivery through various methods, including incorporation into food sources and direct application. Comprehensive testing protocols validate its effectiveness and safety in real-world conditions, demonstrating significant improvements in bee health and survivability. Furthermore, the nanoformulation's environmentally friendly materials and broad applicability extend its potential to protect other pollinators and combat various environmental stressors, thereby contributing to sustainable agriculture and ecosystem health. This invention represents a critical advancement in environmental protection for vital pollinator species. Dated this …….Day of October, 2024 Dr. Monica Gulati Registrar Lovely Professional University

Specification

Description:The following specification particularly describes the invention and the manner it
is to be performed.
TECHNICAL FIELD
[001] The technical field of the inventions encompasses biotechnological approaches for pest control and antimicrobial applications. The first patent focuses on the use of RNA effector molecules and delivery agents to modulate gene expression in insects, aiming to control pest populations and prevent diseases caused by environmental stressors. This involves molecular biology techniques, genetic engineering, and innovative delivery systems tailored for specific insect species. The second patent involves the development of a sterile liquid or gel composition that generates antimicrobial activity through a unique enzyme-substrate system, specifically designed to release hydrogen peroxide in low-water environments. This invention addresses challenges in infection control and environmental disinfection, highlighting applications in medical settings and surface sterilization. Both patents reflect advancements in sustainable agricultural practices and public health solutions.
BACKGROUND
[002] The first invention addresses the growing need for effective pest management strategies in agriculture, particularly in the face of increasing pest resistance to conventional chemical pesticides. Existing methods often rely on broad-spectrum insecticides that can harm non-target species and disrupt ecosystems. Previous patents, such as US10036767B2, which explores genetic modifications in pest insects, demonstrate the potential of using molecular biology for targeted pest control. However, these methods often lack the precision needed to avoid off-target effects and ecological imbalances. The use of RNA effector molecules presents a novel approach to specifically modulate gene expression in pests, offering a targeted and environmentally friendly alternative.
[003] Additionally, the invention recognizes the importance of effective delivery mechanisms for RNA molecules to ensure their stability and uptake in target organisms. Current delivery systems, such as those discussed in US10169108B2, often face challenges in efficiency and specificity. There is a pressing need to develop novel delivery agents that can enhance the bioavailability of RNA molecules while minimizing their impact on beneficial organisms. This patent aims to fill this gap by optimizing delivery methods that can reliably target pest populations while maintaining ecological integrity.
[004] The second invention addresses the critical issue of antimicrobial resistance and the adverse effects of traditional disinfectants on human health and the environment. Existing antimicrobial compositions, such as those highlighted in US10723934B2, often rely on unrefined natural substances, which can introduce variability and potential allergens. This invention proposes a sterile liquid or gel composition that generates hydrogen peroxide through a unique enzyme-substrate system, offering a controlled and consistent antimicrobial action. By excluding unrefined ingredients, the formulation enhances safety and efficacy, making it suitable for various applications, including medical devices and surface disinfection.
[005] Moreover, the low-water content of this composition is a significant advancement, as it improves the stability and shelf life of the product. This is particularly relevant in settings where long-term storage and effectiveness are crucial, such as in healthcare environments. Previous patents have not adequately addressed the challenges of maintaining stability in low-water environments, making this invention a novel contribution to the field of antimicrobial technology.
[006] The potential applications of the inventions extend beyond agriculture and medical settings. The RNA-based pest control methods can be integrated into integrated pest management (IPM) strategies, providing a sustainable alternative to chemical pesticides. Meanwhile, the antimicrobial composition offers versatility in various industries, from food safety to environmental sanitation, addressing a wide range of pathogens while minimizing the risk of developing resistance.
[007] Ultimately, these inventions respond to pressing global challenges related to pest control and infection management. By leveraging innovative biotechnological approaches and addressing existing research gaps, they pave the way for more sustainable practices that align with ecological and public health goals. The combination of targeted RNA technologies and advanced antimicrobial formulations represents a significant advancement in both pest management and infection control.
SUMMARY
[008] The first invention focuses on the use of RNA effector molecules to modulate gene expression in insect pests, providing a targeted approach to pest control and disease prevention. This method addresses the increasing resistance of pests to conventional chemical pesticides, allowing for more environmentally friendly strategies that specifically inhibit growth, reproduction, and survival in targeted insect populations. By harnessing RNA technology, this invention aims to mitigate the adverse effects of traditional pest control methods on non-target species and overall ecosystem health.
[009] To enhance the efficacy of RNA delivery, the invention emphasizes the development of advanced delivery systems. These systems, including nanoparticles and liposomes, are designed to improve the stability, bioavailability, and uptake of RNA molecules in insect tissues. By optimizing these delivery agents, the invention seeks to ensure consistent and effective modulation of gene expression in various insect species, thereby improving the reliability of RNA-based pest control strategies.
[010] Additionally, the invention highlights the importance of conducting extensive field trials to evaluate the real-world applicability of RNA-based methods. By assessing their effectiveness under diverse environmental conditions, the invention aims to establish the long-term impacts of these technologies on insect populations and ecosystems. The focus on cost-effectiveness and scalability further ensures that RNA-based solutions can be widely adopted in agricultural practices, making them accessible to farmers and pest management professionals.
[011] The second invention presents a sterile liquid or gel composition designed to generate antimicrobial activity through the enzymatic release of hydrogen peroxide. This unique composition features a specific enzyme-substrate pairing that operates in a low-water environment, enhancing the stability and efficacy of the product. By deliberately excluding unrefined natural substances, the invention provides a consistent and reliable antimicrobial formulation suitable for various applications, including wound care, medical devices, and surface disinfection.
[012] Overall, both inventions represent significant advancements in their respective fields. The RNA-based pest control methods offer a promising solution to the challenges of pest management in agriculture, while the innovative antimicrobial composition addresses pressing public health concerns related to infection control. Together, these inventions contribute to the development of sustainable practices that prioritize ecological integrity and human health.
BRIEF DESCRIPTION OF THE DRAWINGS
[013] The foregoing detailed description of embodiments is better understood when read in conjunction with the appended drawings. For the purpose of illustrating of the present subject matter, an example of the construction of the present subject matter is provided as figures; however, the invention is not limited to the specific method disclosed in the document and the figures.
[014] The present subject matter is described in detail with reference to the accompanying figures. In the figures, the left-most digit(s) of a reference number identifies the figure in which the reference number first appears. The same numbers are used throughout the drawings to refer to various features of the present subject matter.
[015] Figure 1provides the working prototype of the invention
[016] The given figures depict an embodiment of the present disclosure for illustration and better understanding only.
DETAILED DESCRIPTION
[017] Some of the embodiments of this disclosure, illustrating all its features, will now be discussed in detail. The words "comprising," "having," "containing," and "including," and other forms thereof, are intended to be equivalent in meaning and be open-ended in that an item or items following any one of these words is not meant to be an exhaustive listing of such item or items or meant to be limited to only the listed item or items. It must also be noted that as used herein and in the appended claims, the singular forms "a," "an," and "the" include plural references unless the context clearly dictates otherwise.
[018] The invention harnesses RNA effector molecules to modulate gene expression in target insect species, effectively controlling key biological processes such as growth, reproduction, and survival. By focusing on genes critical to insect life cycles, this method aims to reduce pest populations and limit the spread of diseases caused by pathogens, contributing to enhanced agricultural productivity and ecosystem health.
[019] The methodology involves selecting specific RNA sequences that target essential genes within various insect species. These sequences undergo optimization to ensure maximum efficacy and specificity, minimizing off-target effects that could disrupt non-target organisms. This careful design is critical for developing effective pest control solutions that are both targeted and safe.
[020] In one embodiment it is provided that, the Advanced delivery systems play a pivotal role in the invention, utilizing nanoparticles, liposomes, or viral vectors to enhance the stability and bioavailability of RNA molecules. These delivery agents are engineered to ensure efficient uptake by the target insect tissues, thereby maximizing the treatment's effectiveness while minimizing potential environmental impacts.
[021] Extensive laboratory and field trials are conducted to validate the effectiveness of the RNA-based compositions. These trials assess the treatments under varying environmental conditions, demonstrating significant reductions in pest populations and confirming the practicality of RNA interventions for real-world applications in agriculture and pest management.
[022] In one embodiment it is provided, that the Understanding the mechanisms of resistance that may develop in insect populations is a crucial component of this research. Studies are focused on identifying potential pathways through which insects might adapt to RNA-based treatments, allowing researchers to devise strategies to mitigate resistance and enhance the long-term viability of RNA interventions.
[023] The ecological impact of the RNA delivery agents is also a major area of investigation. Research aims to evaluate the persistence of these agents in ecosystems and their potential effects on non-target species, ensuring that the invention supports environmental sustainability and does not adversely affect beneficial insects or wildlife.
[024] The composition's potential to prevent diseases in insects is another significant aspect. By exploring how RNA interventions can influence the interactions between insects and their pathogens, the invention offers a multifaceted approach to pest management that goes beyond mere population control, enhancing overall insect health.
[025] A key advantage of this invention is its capacity to reduce dependence on chemical pesticides, which can have harmful environmental effects. By providing an innovative, RNA-based solution, the invention promotes a more sustainable approach to pest management, helping to protect both agricultural ecosystems and human health.
[026] The second invention introduces a sterile liquid or gel composition designed to generate antimicrobial activity through a unique enzyme-substrate system that releases hydrogen peroxide. This innovative system operates efficiently in low-water environments, which enhances the stability and effectiveness of the antimicrobial agent over time.
[027] In one embodiment it is provided, that Notably, the formulation deliberately excludes unrefined natural substances such as honey. This exclusion ensures a more consistent and controlled product, reducing variability in performance and minimizing potential allergens, which is particularly important for applications in sensitive medical environments.
[028] Comprehensive testing protocols confirm the broad-spectrum antimicrobial efficacy of the composition against a variety of pathogens, including bacteria, fungi, and viruses. This versatility positions the invention as a valuable solution for applications in wound care, surface disinfection, and other healthcare-related settings where infection control is paramount.
[029] The discussions surrounding the invention emphasize its significance in addressing the growing concern of antimicrobial resistance. By providing an adaptable and effective alternative to traditional antimicrobial agents, the invention supports public health initiatives and sanitation efforts, positioning it as a key tool in modern infection control strategies.
[030] Referring to Figure 1, The image presents a visual representation of the innovative nanoformulation designed to protect honey bees from the detrimental effects of microplastic contamination. It depicts the structure of biodegradable nanoparticles that encapsulate bioactive agents, illustrating their targeted delivery to the bees. The nanoparticles are shown interacting with microplastics, highlighting their role in detoxifying and mitigating the harmful effects of these contaminants on the bees' health. Additionally, the image emphasizes the formulation's compatibility with natural feeding methods, such as incorporation into sugar syrup and pollen. By illustrating both the composition and the mechanisms of action, the image effectively conveys the potential of this nanoformulation to enhance honey bee resilience against environmental stressors, thereby contributing to their well-being and, ultimately, to sustainable agricultural practices.
Dated this …….Day of October, 2024
Dr. Monica Gulati
Registrar
Lovely Professional University

, Claims:We claim:
1. A nanoformulation for protecting honey bees from microplastic contamination, comprising:
A. biodegradable nanoparticles encapsulating bioactive agents, selected from antioxidants, detoxifying enzymes, and chelating agents, designed for targeted and controlled release;
B. a delivery system that allows incorporation of the nanoformulation into food sources such as sugar syrup or pollen;
C. a mechanism to mitigate harmful interactions between microplastics and honey bees' digestive systems, reducing oxidative stress and improving overall health.
2. The nanoformulation of claim 1, wherein the biodegradable nanoparticles are made from materials selected from the group consisting of polylactic acid (PLA), polycaprolactone (PCL), and starch-based polymers, providing environmentally friendly options that degrade over time and reduce environmental impact.
3. The nanoformulation of claim 1, wherein the antioxidants are selected from the group consisting of vitamin E, ascorbic acid, and polyphenols, which are incorporated to combat oxidative stress caused by microplastic ingestion, enhancing the health and longevity of honey bees.
4. The nanoformulation of claim 1, wherein the detoxifying enzymes include superoxide dismutase and catalase, which work to neutralize harmful reactive oxygen species generated from microplastic exposure, thereby improving the overall physiological response in honey bees.
5. The nanoformulation of claim 1, wherein the chelating agents are selected from the group consisting of EDTA and citric acid, which are effective in binding heavy metals and toxins associated with microplastics, further aiding in detoxification and promoting bee health.
6. The nanoformulation of claim 1, wherein the delivery system is designed to enhance the bioavailability of the bioactive agents when administered to honey bees, ensuring that the active components are effectively absorbed and utilized within the bees' digestive systems for maximum therapeutic effect.
7. The nanoformulation of claim 1, wherein the formulation reduces microplastic-induced digestive blockages in honey bees by at least 50%, demonstrating significant efficacy in alleviating one of the critical health issues linked to microplastic contamination.
8. The nanoformulation of claim 1, wherein the overall health of honey bees, as measured by survival rates and reproductive success, is improved by at least 30% following treatment with the formulation, highlighting the potential for enhanced bee population sustainability in the face of environmental stressors.
Dated this …….Day of October, 2024

Dr. Monica Gulati
Registrar
Lovely Professional University

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