NOVEL SURGICAL SILK SUTURE COATING WITH GRAPHENE OXIDE NANOPARTICLES LOADED WITH RESVERATROL

Abstract

ABSTRACT: The development of infection-resistant surgical sutures remains a significant challenge in modem periodontal surgery. This presents a novel silk suture coating incorporating graphene oxide nanoparticles (GO NPs) loaded with resveratrol, aimed at enhancing the healing process and reducing infection risk in surgical applications. The coating utilizes the unique properties of graphene oxide, including high surface area and biocompatibility, to effectively deliver resveratrol, a natural compound known for its antioxidant and anti-inflammatory effects. The graphene oxide nanoparticle silk suture coating represents a significant advancement in surgical technology, integrating mechanical robustness with bioactive properties to facilitate improved healing outcomes. This innovative approach could lead to safer surgical practices, reduced complication rates, and enhanced patient recovery in various clinical settings.

Specification

FORM - 2
THE PATENTS ACT, 1970
(39 OF 1970)
&
THE PATENTS RULES, 2003
COMPLETE SPECIFICATION
(see Section 10 & rule 13)

1. TITLE OF THE INVENTION: Novel surgical silk suture coating with graphene oxide nanoparticles loaded with resveratrol.

S.No NAME NATIONALITY ADDRESS
1 Saveetha Institute of Medical
and Technical Sciences
INDIAN
No: 162, Poonamallee High
Road, Velappanchavadi,
Chennai-600077

3. PREAMBLE TO THE DESCRIPTION:
The following specification describes the invention and how it is to be performed.

Bank : INDUSIND BANK
Branch : NUNGAMBAKKAM
( SAVEETHA UNIVERSITY )
DD No : 402481
DD Date: 19/10/2024
DD. Amount; 8900/-

TITLE OF INVENTION :
Novel surgical silk suture coating with graphene oxide nanoparticles loaded with resveratrol.

FIELD OF INVENTION :
The invention relates to a suitable surgical silk suture coating with graphene oxide nanoparticles loaded with resveratrol for periodontal
surgeries .

BACKGROUND:

Surgical site infections remain a significant challenge in healthcare, necessitating ongoing research and the implementation of effective
preventive measures. Innovations in surgical materials, such as antimicrobial coatings on sutures and other devices, are promising
strategies to reduce the incidence of SSIs and improve patient outcomes. Reducing SSIs is crucial for enhancing surgical safety, reducing
healthcare costs, and improving overall patient satisfaction.

Silk sutures have been widely used in surgical procedures due to their favorable mechanical properties, biocompatibility, and ability to promote tissue integration. However, they are susceptible to bacterial colonization,
which can lead to infections and delayed healing. This concern is particularly pressing in the context of increasing antibiotic resistance,
underscoring the need for alternative approaches to enhance the efficacy of sutures. The development of innovative coatings that incorporate therapeutic agents represents a promising strategy to overcome these limitations.

Graphene oxide (GO) nanoparticles are a derivative of graphene characterized by their two-dimensional structure and functional groups that allow for easy modification. Their unique properties-including high surface area, mechanical strength, and biocompatibility-make them an attractive candidate for biomedical applications. GO can be loaded with various therapeutic agents, facilitating targeted delivery and sustained
release.

Resveratrol is a natural polyphenolic compound found in various plants, particularly in grapes and berries. It is renowned for its antioxidant, antiinflammatory, and antimicrobial properties. Resveratrol has been shown to promote wound healing by enhancing angiogenesis, reducing
inflammation, and stimulating cellular proliferation. However, its clinical use is limited by its low bioavailability and rapid degradation.

The integration of resveratrol into GO nanoparticles creates a synergistic effect that leverages the strengths of both materials. The graphene oxide acts as a stable carrier for resveratrol, improving its stability and allowing
for controlled release. This combination not only provides antimicrobial activity against common pathogens but also promotes tissue regeneration
through the bioactive effects of resveratrol.

SUMMARY OF INVENTION :
This invention introduces a novel silk suture coating that incorporates graphene oxide nanoparticles (GO NPs) loaded with resveratrol, designed to enhance wound healing and reduce the risk of surgical site infections.

Known for their excellent tensile strength and biocompatibility, silk sutures are widely used in surgical applications but are prone to bacterial
colonization. Graphene Oxide Nanoparticles are engineered from graphene oxide, boasting properties such as high surface area, mechanical
strength, and biocompatibility. They serve as carriers for therapeutic agent. A natural polyphenolic compound with antioxidant, antiinflammatory, and antimicrobial properties. It enhances healing and reduces inflammation.

The incorporation of GO enhances the antibacterial efficacy of the suture, reducing the risk of infections. The controlled release of resveratrol can enhance local tissue regeneration, promoting faster healing and reducing inflammation.

The addition of reduced graphene oxide (rGO) to silk enhances its antioxidant activity. rGO has properties that can contribute to free radical
scavenging. Combining resveratrol with rGO on silk provides the highest antioxidant activity. Resveratrol is a potent antioxidant, and its
combination with rGO synergistically enhances the overall antioxidant potential

The combination of silk and GO provides a robust suture that maintains its strength while delivering therapeutic agents. Both silk and graphene oxide are biocompatible, minimizing adverse reactions and promoting healing.

This picture depicts surgical silk suture coating with graphene oxide nanoparticles loaded with resveratrol.

CLAIMS OF THE INVENTION :

1. A silk suture coated with graphene oxide nanoparticles (GO NPs) loaded with resveratrol, wherein the GO NPs are uniformly distributed across the silk surface to enhance antimicrobial and healing properties.

2. The coated silk sutures demonstrate significant antibacterial activity against common pathogens associated with surgical site infections,
including Staphylococcus aureus and Escherichia coli.

3. The combination of silk and GO NPs provides enhanced mechanical strength, flexibility and biocompatibility ensuring that the sutures
maintain their structural integrity during the healing process.

4. Resveratrol is a potent antioxidant, and its combination with rGO synergistically enhances the overall antioxidant potential.

5. The novel coating significantly decreases the incidence of surgical site infections and complications, leading to faster patient recovery
and reduced healthcare costs.

REFERENCE :
28-Oct-2024/130784/202441082181/Form 2(Title Page)
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Lewis BD, Brown ICR, Towne JB. Bacterial adherence to surgical
sutures: can antibacterial-coated sutures reduce the risk of
microbial contamination?. Journal of the American College of
Surgeons. 2006 Oct 1 ;203(4):481-9.
2. Oksiiz KE, Kurt B, §ahin Inan ZD, Hepokur C. Novel bioactive
glass/graphene oxide-coated surgical sutures for soft tissue
regeneration. ACS omega. 2023 Jun 8;8(24):21628-41.
3. Grant JJ, Pillai SC, Hehir S, McAfee M, Breen A. Biomedical
applications of electrospun graphene oxide. ACS Biomaterials
Science & Engineering. 2021 Mar 17;7(4): 1278-301.
4. Haley RM. Dual Delivery of Anti-Inflammatory and Anti-
Microbial Drugs from Affinity Polymer Coated Sutures Parallels
Wound Healing Timeline (Master's thesis, Case Western Reserve
University).
5. Lakshmanan R, Campbell J, Ukani G, Beringhs AO, Selvaraju V,
Thirunavukkarasu M, Lu X, Palesty JA, Maulik N. Evaluation of
dermal tissue regeneration using resveratrol loaded fibrous matrix
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