MULTIMEDIA SLIDE CULTURE PLATE FOR SIMULTANEOUS DETECTION OF MONOFUNGAL SPORE MORPHOLOGY

Abstract

ABSTRACT OF THE INVENTION The study presents a new square-shaped plate that is specifically developed to improve the precision and effectiveness of identifying the shape and structure of fungal spores using the slide culture technique. Conventional fungal culture methods typically involve using many plates, each containing a specific type of medium. This results in higher consumption of resources, longer time requirements, and an increased likelihood of experimental variance. This novel plate overcomes these constraints by allowing the concurrent growing of fungus on three distinct media within a singular unit. The plate is carefully designed to fit three standard microscope slides, each with its own partition, to ensure that the growth of fungi on each slide remains separate. This design effectively reduces the risk of cross-contamination, enabling a straightforward comparison of fungal growth on various media. With the addition of an inner top projection that snugly fits into the bottom of the plate, the culture environment is further strengthened, guaranteeing that the slides stay securely in place during the cultivation process. This plate's main advantage is its ability to enable a thorough examination of fungal growth in different media conditions with just one experimental setup. Scientists can now analy;(e how various nutritional environments impact spore morphology and fungal development simultaneously, greatly enhancing the effectiveness of experimental processes. This design also allows for efficient use of laboratory resources, minimizing the requirement for multiple plates and other consumables.

Specification

BACKGROUND OF THE INVENTION
Understanding fungal spore morphology is essential in the fields of mycology, microbiology,
and biotechnology. Precise recognition and comprehension of fungal species are crucial for a
range of purposes, such as disease detection, agricultural control, and industrial operations.
Conventional techniques for studying fungi typically require the use of numerous culture plates,
each containing a specific type of medium. This method, although highly efficient, requires a
significant number of resources, takes up a considerable amount of time, and is susceptible to
differences between samples because of the need to use different plates for various media.
Observing and comparing the growth and morphological characteristics of fungi across
different media conditions within a single experimental setup can significantly improve the
efficiency and accuracy of fungal research. Developing a new plate that allows for the
simultaneous cultivation of fungi on multiple media using the slide culture technique has
become necessary to improve efficiency and conserve resources. This design enables a holistic
comprehension of the impact of different media on fungal growth, morphology, and spore
formation, all within a single, controlled environment.
PRIOR ART:
An apparatus described in U.S. Patent No. 5,252,468, issued on October 12, 1993, and assigned
to Becton Dickinson and Company, is designed for the cultivation and observation of fungi.
The apparatus consists of a chamber equipped with a nutrient medium and a support structure
designed to securely hold a fungal sample. This invention aims to establish a controlled
environment that enables precise observation of fungal growth, including spore formatio-n and
mycelial development
This patent, U.S. Patent No. 6,475,753, focuses on a specialized Petri dish created by Millipore
Corporation. The dish is designed to enable the cultivation of multiple microbial samples
simultaneously, with each sample having its own dedicated compartment within the dish. The
patent was issued on November 5, 2002. The compartments are separated by raised dividers,
ensuring a strict separation between samples to prevent any cross-contamination. This design is
particularly useful for general microbial cultures, but it also has great potential in fungal
research. Although it has compartments, the design does not include specific areas for placing
slides for fungal cultivation.

SUMMARY OF THE INVENTION:
This invention presents a square-shaped culture plate that aims to greatly improve the precision
and effectiveness of detecting fungal spore morphology. It is titled "Multimedia Slide Culture
Plate for Simultaneous Detection of Monofungal. Spore Morphology." This innovative plate
addresses the shortcomings of conventional fungal culture methods, which frequently
necessitate the utilization of numerous plates, each featuring a solitary variety of medium.
These conventional approaches require a significant number of resources, take up a lot of time,
and are prone to experimental inconsistencies.
This plate's design enables the cultivation of fungi on three distinct types of media
simultaneously, all within a single unit. This method helps to decrease the necessity for
numerous plates, thus saving resources and reducing the time needed for fungal culture. In
addition, housing various media in a single unit helps minimize experimental variation and
contamination, resulting m more consistent and reliable results.
This invention provides a streamlined, efficient, and accurate method for detecting and studying
monofungal spore morphology, making it a valuable tool for laboratories engaged in fungal
research and diagnostics.






CLAIMS
Claim 1: The culture plate is a square-shaped culture plate that has been thoughtfully designed
for convenient handling during fungal cultivation. The compact and ergonomic design of this
product improves user convenience and stability when preparing and observing fungal
cultures.
Claim 2: The culture plate has three separate partitions, each specifically made to hold a slide
containing agar blocks of different media. This setup enables the concurrent cultivation of
fungi on various media, making it easier to conduct comparative analysis within a single
experiment.
Claim 3: The culture plate has an upper lid that is carefully designed to perfectly align with
the partitioned bottom section. This design effectively prevents any cross-contamination
between compartments, guaranteeing the isolation and purity of each fungal culture throughout
the growth process.
Claim 4: This fungal cultivation plate ensures a consisteiltly regulated environment throughout
all partitions. By carefully observing and analyzing the effects of various media on fungal
growth, we can ensure that our experimental n:sults are reliable and consistent.
Claim
Claim 5: A fungal culture plate that is user-friendly and convenient to handle. The integrated
design of this product streamlines the process of preparing, observing, and transporting fungal
cultures, resulting in improved efficiency in the laboratory.

Documents