MODIFIED ESTERASE

Abstract

The present invention provides any of the following modified esterases (i) to (iii): (i) a modified esterase including an amino acid sequence in which at least one amino acid in a position selected from the group consisting of (1) to (24) in the amino acid sequence of SEQ ID NO: 1 or 3 is substituted with another amino acid, said group consisting of (1) V64, (2) S65, (3) A102, (4) A109, (5) L151, (6) I153, (7) Y173, (8) Y197, (9) L200, (10) I211, (11) F217, (12) S220, (13) D222, (14) D227, (15) L229, (16) L242, (17) A245, (18) V257, (19) L298, (20) T301, (21) V326, (22) S329, (23) A350, and (24) T371; (ii) the modified esterase of (i), wherein one or more amino acids are additionally substituted (excluding the amino acid position substituted in (i)), added, inserted, or deleted (excluding the amino acid position substituted in (i)) and the reactivity to (1R,3R)-Methyl 3-(2-chloro-3,3,3-trifluoroprop-1-en-1-yI)-2,2-dimethylcyclopropane-1-carboxylate is improved compared to the reactivity of an esterase comprising the amino acid sequence of SEQ ID NO: 1 or 3; and (iii) the modified esterase of (i), wherein an amino acid other than the amino acid substituted in (i) is additionally substituted with another amino acid, the modified esterase has at least 70% homology with the modified esterase of (i), and the reactivity to (1R,3R)-Methyl 3-(2-chloro-3,3,3-trifluoroprop-1-en-1-yI)-2,2-dimethylcyclopropane-1-carboxylate is improved compared to the reactivity of an esterase comprising the amino acid sequence of SEQ ID NO: 1 or 3.

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