METHOD FOR DRUG QUANTIFICATION USING THIN LAYER CHROMATOGRAPHY

Abstract

ABSTRACT A method (100) for drug quantification using thin layer chromatography, the method (100) comprising: preparing standard solutions of different concentrations of 4-Acetamidophenone and Brucine free base; spotting 50 µl of each concentration on a TLC plate; developing the TLC plate using specified solvent systems; visualizing the developed spots using a web application for TLC analysis; and validating the method based on ICH guidelines.

Specification

Description:FIELD OF THE DISCLOSURE
[0001] This invention generally relates to a field of quantifying drug compounds, and more specifically, to a method for using thin layer chromatography (TLC) and a web application for the precise quantification of 4-Acetamidophenone and Brucine free base in various concentrations.
BACKGROUND

[0002] The subject matter discussed in the background section should not be assumed to be prior art merely as a result of its mention in the background section. Similarly, a problem mentioned in the background section or associated with the subject matter of the background section should not be assumed to have been previously recognized in the prior art. The subject matter in the background section merely represents different approaches, which in and of themselves may also correspond to implementations of the claimed technology.
[0003] Traditional methods of drug quantification, such as High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC), often require complex instrumenta , Claims:1. A method (100) for drug quantification using thin layer chromatography, the method (100) comprising:
preparing standard solutions of different concentrations of 4-Acetamidophenone and Brucine free base;
spotting 50 µl of each concentration on a TLC plate; developing the TLC plate using specified solvent systems; and
visualizing the developed spots using a web application for TLC analysis; and validating the method based on ICH guidelines.

2. The method (100) as claimed in claim 1, wherein the specified solvent systems are ethyl acetate: methanol: ammonia (85:15:05) for 4-Acetamidophenone and methanol: ammonia (90:10) for Brucine free base

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