METHOD FOR CHARACTERIZING BACTERIOPHAGES

Abstract

ABSTRACT A method (100) for characterizing bacteriophages comprising the steps of collecting sewage water samples from a natural source, processing the sewage samples with a phage buffer solution to obtain viable and concentrated solution of bacteriophages, isolating the bacteriophages from the concentrated solution of bacteriophages by performing a double-layer agar plaque assay, determining the bacteriophage-induced plaque formations based on the double-layer agar plaque assay and characterising genetic material of bacteriophages of the bacteriophage-induced plaque formations.

Specification

Description:FIELD OF THE DISCLOSURE
[0001] This invention generally relates to a field bacteriophages and in particular relates to a method for characterising bacteriophages.
BACKGROUND

[0002] The subject matter discussed in the background section should not be assumed to be prior art merely as a result of its mention in the background section. Similarly, a problem mentioned in the background section or associated with the subject matter of the background section should not be assumed to have been previously recognized in the prior art. The subject matter in the background section merely represents different approaches, which in and of themselves may also correspond to implementations of the claimed technology.
[0003] The rapid rise of multi-drug resistant (MDR) bacterial infections has posed significant challenges in treating common bacterial diseases, resulting in increased mortality, prolonged hospital stays, and substantial healthcare costs. Traditional antibiotic treatments are increasingly ineffective against M , Claims:1. A method (100) for characterizing bacteriophages, the method (100) comprising the steps of:
collecting sewage water samples from a natural source;
processing the sewage samples with a phage buffer solution to obtain viable and concentrated solution of bacteriophages;
isolating the bacteriophages from the concentrated solution of bacteriophages by performing a double-layer agar plaque assay;
determining the bacteriophage-induced plaque formations based on the double-layer agar plaque assay; and
characterising genetic material of bacteriophages of the bacteriophage-induced plaque formations.

2. The method (100) as claimed in claim 1, wherein the bacteriophage-induced plaque formations exhibit lytic activity against target multi-drug resistant bacteria, including at least Staphylococcus aureus and Pseudomonas aeruginosa.

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