METHOD FOR BIOFUEL PRODUCTION

Abstract

ABSTRACT A method (100) for biofuel production, the method (100) comprising the steps of isolating a microalgae strain from an aquatic environment using nutrient-enriched media, sub-culturing the microalgae strain to obtain a microalgae biomass, increasing lipid content of the microalgae biomass by optimizing growth conditions in a wastewater-based cultivation and applying a lipid extraction technique selected from chemical or mechanical methods to obtain a biofuel.

Specification

Description:FIELD OF THE DISCLOSURE
[0001] This invention generally relates to a field of biofuels and in particular relates to a method for biofuel production using microalgae biomass.
BACKGROUND

[0002] The subject matter discussed in the background section should not be assumed to be prior art merely as a result of its mention in the background section. Similarly, a problem mentioned in the background section or associated with the subject matter of the background section should not be assumed to have been previously recognized in the prior art. The subject matter in the background section merely represents different approaches, which in and of themselves may also correspond to implementations of the claimed technology.
[0003] Traditionally, biofuel production has relied on sources such as crop-based feed stocks, including corn, soy, and palm oil. While these sources are capable of yielding biofuel, their use raises concern about land use, food security, and environmental impact. Moreover, traditional biofuel sourc , Claims:1. A method (100) for biofuel production, the method (100) comprising the steps of:
isolating a microalgae strain from an aquatic environment using nutrient-enriched media;
sub-culturing the microalgae strain to obtain a microalgae biomass;
increasing lipid content of the microalgae biomass by optimizing growth conditions in a wastewater-based cultivation; and
applying a lipid extraction technique selected from chemical or mechanical methods to obtain a biofuel.

2. The method (100) as claimed in claim 1, wherein the growth conditions include temperature, pH, and nutrient concentration.

3. The method (100) as claimed in claim 1, wherein the chemical method includes Soxhlet extraction or supercritical fluid extraction.

4. The method (100) as claimed in claim 1, wherein the mechanical method includes ultrasonic-assisted or microwave-assisted extraction.

5. The method (100) as claimed in claim 1, further comprising drying the microalgae biomass prior to extraction to improve lipid yield and quality of the b

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