IMAGE CAPTURING DEVICE FOR DIGITAL COLORIMETRIC ANALYSIS IN A MICROFLUIDIC DEVICE

Abstract

Embodiments of the present disclosure relate to an image capturing device (100) for digital colorimetric analysis in a microfluidic device. The image capturing device (100) includes an upper section (102) comprising a housing (108) for enclosing a plurality of LED lights, a LED screen, a plurality of camera sensors configured to capture images of the microfluidic samples from multiple angles, and a microcontroller. The microcontroller is configured to perform digital colorimetric detection of biomolecules on the images and obtain corresponding concentration values based on the digital colorimetric detection. The image capturing device (100) also includes a lower section for housing a microfluidic chip holder to securely position a microfluidic chip (112) and a positioning slider (110) for ensuring optimal focus and alignment for accurate colorimetric measurements. The image capturing device (100) is configured to capture a plurality of images thus enabling simultaneous colorimetric detection for a plurality of analytes.

Specification

Description:TECHNICAL FIELD
[0001] The present disclosure relates to the field of microfluidic detection devices. More particularly, the present disclosure relates to an image capturing device for digital colorimetric analysis in a microfluidic device.

BACKGROUND
[0002] There has been significant interest and demand in designing and developing microfluidic detection devices, particularly for Point-of-Care (POC) diagnostic applications. Colorimetric detection is ideal for POC diagnostics due to its simplicity, low cost, and quick, visually interpretable results, requiring minimal training and no advanced equipment. Its portability, ability to operate without external power, and potential for high specificity and multiplexing make it especially valuable in low-resource or remote healthcare settings. In line with this, a glass microfluidic device with a multi-layered polyvinyl chloride film has been filed for an Indian patent. This microfluidic device serves as a platform for sample collection, reagent mixing, and facilitating the colorimetric reaction.
[0003] For point-of-care (POC) colorimetric detection, two key components come into play. The first is the reaction platform/ microfluidic device, where the chemical reaction occurs, and the second is the image capturing equipment, which facilitates the actual colorimetric detection. Flatbed scanners have been widely explored as alternatives for colorimetric detection in POC setups. However, a major drawback of office scanners is their lack of portability and the need for trained personnel to scan data and manually operate the scanner software. Smartphones, on the other hand, are more suitable for POC testing due to their portability, powerful computing capabilities, and high-resolution cameras capable of quantifying colour intensity. Despite their potential, there are some concerns with using smartphones for digital image colorimetry. Data obtained from scanners and smartphone cameras are often pre-processed by the device's software, which can interfere with the extraction of raw data. This preprocessing can result in device-dependent variations in colorimetric readouts of microfluidic paper-based analytical devices (µPADs), potentially affecting the results. Another challenge with smartphones is the inconsistency in image capture parameters across different brands when using the built-in auto camera. Factors such as exposure time, light sensitivity, and white balance can significantly impact the baseline signal of images. Colour intensity in digital images is also influenced by the lighting conditions during image capture. As a result, imaging under different lighting conditions in the field, compared to a laboratory calibration curve, can lead to inaccurate results. To address this, several studies have introduced the use of a plastic box with smartphones to control lighting conditions, imaging angles, shadows, and focal distances. These boxes, often produced through additive manufacturing to minimize cost, typically contain an LED light source, a microprocessor, electronic circuits to power the light, slots for inserting the microfluidic test device, and windows for placing the smartphone camera. However, design flexibility becomes a challenge when accommodating smartphones of varying sizes and shapes.
[0004] To address these limitations, the present invention provides a novel device and method that overcome the shortcomings of the prior art.

OBJECTS OF THE PRESENT DISCLOSURE
[0005] Some of the objects of the present disclosure, which at least one embodiment herein satisfies are as listed herein below.
[0006] It is a primary object of the present disclosure to provide an image capturing device for digital colorimetric analysis that is compatible with glass microfluidic device fabricated by layers of poly-vinyl film stacked on top of each other.
[0007] It is another object of the present disclosure to provide an image capturing device for digital colorimetric analysis that enables multiple colorimetric detections on a single microfluidic chip.
[0008] It is yet another object of the present disclosure to provide an image capturing device for digital colorimetric analysis that is enabled to capture subtle colour changes in the microfluidic channels, allowing for precise quantification of analyte concentration even at low levels making it ideal for detecting minimal colorimetric shifts.
[0009] It is another object of the present disclosure to provide an image capturing device for digital colorimetric analysis in a microfluidic device that enables real-time observation of colour changes which allows for immediate feedback and assessment, reducing wait times and facilitating quicker decision-making in experimental workflows.
[0010] It is yet another object of the present disclosure to provide an image capturing device for digital colorimetric analysis in a microfluidic device that includes automated alignment, focus adjustment, and programmable settings, allowing for reproducible results across multiple tests which reduces user intervention, minimizing human error and enhancing data consistency.
[0011] It is yet another object of the present disclosure to provide an image capturing device for digital colorimetric analysis in a microfluidic device that eliminates device dependent image capture variability.

SUMMARY
[0012] The present disclosure relates to the field of microfluidic detection devices. More particularly, the present disclosure relates to an image capturing device for digital colorimetric analysis in a microfluidic device.
[0013] In an aspect of the present disclosure, an image capturing device for digital colorimetric analysis in a microfluidic device is disclosed. The image capturing device includes an upper section that includes a housing for enclosing a plurality of LED lights configured to provide uniform illumination across microfluidic samples enabling precise quantification of colour changes related to analyte concentration, a LED screen configured to display real-time images of the microfluidic samples, a plurality of camera sensors configured to capture images of the microfluidic samples from multiple angles, and a microcontroller, operatively coupled with the plurality of camera sensors. The microcontroller is configured to obtain the images of the samples from the plurality of camera sensors. The microcontroller is configured to process the images for extraction of data. The microcontroller is configured to perform digital colorimetric detection of biomolecules on the processed images. The microcontroller is configured to obtain corresponding concentration values based on the digital colorimetric detection. The image capturing device further includes a lower section that includes a microfluidic chip holder configured to securely position a microfluidic chip ensuring precise alignment and stable illumination for accurate colorimetric analysis and a positioning slider configured to enable fine adjustments to positioning of the microfluidic chip ensuring optimal focus and alignment for accurate colorimetric measurements. The image capturing device is configured to capture a plurality of images thus enabling simultaneous colorimetric detection for a plurality of analytes.
[0014] In an aspect of the present disclosure, a method of using the image capturing device for digital colorimetric analysis in the microfluidic device is disclosed. The method begins with obtaining, by the microcontroller, the images of the samples from the plurality of camera sensors. The method proceeds with processing the images, by the microcontroller, for extraction of data. The method proceeds with performing, by the microcontroller, digital colorimetric detection of biomolecules on the processed images. The method ends with obtaining, by the microcontroller, corresponding concentration values based on the digital colorimetric detection.

BRIEF DESCRIPTION OF DRAWINGS
[0015] The accompanying drawings are included to provide a further understanding of the present disclosure and are incorporated in, and constitute a part of this specification. The drawings illustrate exemplary embodiments of the present disclosure, and together with the description, serve to explain the principles of the present disclosure.
[0016] In the figures, similar components, and/or features may have the same reference label. Further, various components of the same type may be distinguished by following the reference label with a second label that distinguishes among the similar components. If only the first reference label is used in the specification, the description applies to any one of the similar components having the same first reference label irrespective of the second reference label.
[0017] Fig. 1 illustrates an exemplary three-dimensional rendered representation of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0018] Fig. 2 illustrates an exemplary representation of a top view of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0019] Fig. 3 illustrates an exemplary representation of a top view of a lower section of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0020] Fig. 4A-4B illustrates exemplary representations of an upper surface and a lower surface of a microfluidic chip holder and a positioning slider of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0021] Fig. 5 illustrates an exemplary representation of a battery casing and cover plate of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0022] Fig. 6 illustrates an exemplary flow representation of a method of using the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.

DETAILED DESCRIPTION
[0023] The following is a detailed description of embodiments of the disclosure depicted in the accompanying drawings. The embodiments are in such detail as to communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments. On the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit, and scope of the present disclosure as defined by the appended claims.
[0024] In an embodiment of the present disclosure, an image capturing device for digital colorimetric analysis in a microfluidic device is disclosed. The image capturing device includes an upper section that includes a housing for enclosing a plurality of LED lights configured to provide uniform illumination across microfluidic samples enabling precise quantification of colour changes related to analyte concentration, a LED screen configured to display real-time images of the microfluidic samples, a plurality of camera sensors configured to capture images of the microfluidic samples from multiple angles, and a microcontroller, operatively coupled with the plurality of camera sensors. The microcontroller is configured to obtain the images of the samples from the plurality of camera sensors. The microcontroller is configured to process the images for extraction of data. The microcontroller is configured to perform digital colorimetric detection of biomolecules on the processed images. The microcontroller is configured to obtain corresponding concentration values based on the digital colorimetric detection. The image capturing device further includes a lower section that includes a microfluidic chip holder configured to securely position a microfluidic chip ensuring precise alignment and stable illumination for accurate colorimetric analysis and a positioning slider configured to enable fine adjustments to positioning of the microfluidic chip ensuring optimal focus and alignment for accurate colorimetric measurements. The image capturing device is configured to capture a plurality of images thus enabling simultaneous colorimetric detection for a plurality of analytes.
[0025] In an aspect, the plurality of LED lights is configured to provide consistent and controllable illumination across the microfluidic chip allowing even light distribution and minimizing shadows or glare that could distort colour readings.
[0026] In an aspect, the LED screen is configured to overlay analytical data directly on the captured images which enhances accuracy of colorimetric readings and enables real-time assessment of quantitative results.
[0027] In an aspect, the plurality of camera sensors is optimized to capture specific colour wavelengths corresponding to LED illumination, ensuring that even subtle colour variations are recorded accurately.
[0028] In an aspect, the microcontroller is configured to apply deep learning techniques to process the images captured by the plurality of camera sensors.
[0029] In an aspect, the microfluidic chip holder is compatible with a glass microfluidic device with a multi-layered polyvinyl chloride film.
[0030] In an aspect, the microfluidic chip holder is configured to lock the microfluidic chip into a specific plane relative to the plurality of camera sensors and the plurality of LED lights, ensuring that the microfluidic chip remains flat and parallel to the plurality of LED lights, which is essential for achieving even illumination and accurate imaging across a sample area.
[0031] In an aspect, the microfluidic chip holder is configured to work in conjunction with the positioning slider, enabling fine-tuned adjustments in all three dimensions (X, Y, and Z axes) to achieve optimal focus and alignment with image capturing optics.
[0032] In an aspect, the microcontroller is powered by an AC adapter and a battery pack.
[0033] In an embodiment of the present disclosure, a method of using the image capturing device for digital colorimetric analysis in the microfluidic device is disclosed. The method begins with obtaining, by the microcontroller, the images of the samples from the plurality of camera sensors. The method proceeds with processing the images, by the microcontroller, for extraction of data. The method proceeds with performing, by the microcontroller, digital colorimetric detection of biomolecules on the processed images. The method ends with obtaining, by the microcontroller, corresponding concentration values based on the digital colorimetric detection.
[0034] The various embodiments throughout the disclosure will be explained in more detail with reference to FIGs. 1-6.
[0035] FIG. 1 illustrates an exemplary three-dimensional rendered representation of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0036] Illustrated in FIG. 1 is an image capturing device 100 for digital colorimetric analysis in a microfluidic device. The image capturing device 100 may include an upper section 102 and a lower section 104. There is also provided a top cover 106 with magnetic latches. The top cover 106 with the magnetic latches in the image capturing device 100 multiple practical and protective functions. Primarily, the top cover 106 securely encloses the microfluidic chip and internal components, to provide protection from dust, ambient light, and external contaminants that could interfere with image quality and colorimetric readings. The magnetic latches allow for quick and easy access, enabling the user to insert and remove microfluidic chips efficiently without the need for complex latching mechanisms. By blocking ambient light, the top cover 106 helps maintain controlled lighting conditions ensuring uniform illumination across the sample. This controlled environment is critical for accurate colour measurements, eliminating interference from external light sources. Additionally, the secure closure provided by the magnetic latches minimizes vibrations and movement during image capture, enhancing the stability and consistency of data collection. The magnetic design also reduces wear and tear, increasing the longevity of the image capturing device 100 by providing a reliable and user-friendly method for repeated access.
[0037] In an embodiment of the present disclosure, the upper section 102 of the image captioning device 100 includes a housing 108 for enclosing a plurality of LED lights, a LED screen, a plurality of camera sensors, and a microcontroller.
[0038] The plurality of LED lights in the image capturing device 100 plays a critical role in providing precise and controlled illumination essential for accurate colour measurements. The plurality of LED lights emits light across specific wavelengths (often red, green, blue, or even near-infrared), matching the colorimetric requirements of the assay to enhance the detection of subtle colour changes in the microfluidic sample. Uniform illumination across the sample area is ensured by careful positioning of the plurality of LED lights, which reduces shadows and glare, eliminating potential distortions in colour representation.
[0039] By providing consistent, stable light intensity, the plurality of LED lights enables the image capturing device 100 to capture reproducible data across multiple samples and experiments. The intensity of the plurality of LED lights may often be modulated, allowing for adjustments to accommodate different types of samples or assays with unique illumination needs. This flexibility ensures optimal lighting conditions for each measurement, enhancing sensitivity and accuracy in detecting slight colour variations, which may correspond to small changes in analyte concentration.
[0040] The plurality of LED lights may be synchronized with the plurality of camera sensors, allowing for rapid, timed illumination that captures transient colour changes in kinetic assays. This synchronization improves temporal resolution, ensuring that even fast or dynamic reactions are captured accurately. The plurality of LED lights creates a controlled, adaptable lighting environment that is vital for high-quality, reliable colorimetric analysis in microfluidic applications.
[0041] The LED screen in the image capturing device 100 serves as a vital interface, providing real-time visual feedback of the microfluidic sample for accurate monitoring and analysis. By displaying high-resolution images of the sample, the LED screen allows the user to observe colour changes directly in real-time, facilitating immediate assessment of reactions and assay progress. This is especially useful for time-sensitive analyses where rapid detection of colour shifts is critical to obtaining accurate results. Beyond simple display functions, the LED screen often includes overlays of analytical metrics, such as RGB values, intensity histograms, or other colorimetric data, which enhance quantification and interpretation of colour changes in real time. This immediate access to data ensures that any deviations, irregularities, or unexpected changes in the sample can be quickly identified and addressed. The LED screen may also support interactive controls, allowing the user to adjust parameters including focus, lighting, and exposure directly, optimizing image quality and ensuring precise colour measurements.
[0042] Furthermore, the LED screen acts as a convenient platform for calibration and setup of the image capturing device 100, guiding the user through alignment and positioning of the microfluidic chip for consistent results across different runs. By offering an intuitive interface, the LED screen simplifies operation and enables efficient troubleshooting, contributing to the ease of use and reliability of the image capturing device 100. In sum, the LED screen is an essential tool for real-time visualization, data interpretation, and parameter adjustment, supporting accurate and repeatable colorimetric analysis in microfluidic applications
[0043] In an embodiment of the present disclosure, the plurality of camera sensors in the image capturing device 100 provides high-resolution, multi-angle imaging that significantly enhances the accuracy and reliability of colorimetric measurements within a microfluidic setup. By strategically positioning the plurality of camera sensors around the microfluidic chip, the image capturing device 100 captures images from different perspectives, which reduces the likelihood of shadows or reflections distorting the colour data. This setup ensures that the entire sample area is evenly analysed, regardless of the geometry or the lighting angle of the microfluidic chip, leading to more precise and consistent colorimetric readings.
[0044] Each of the plurality of camera sensors may be optimized to capture specific wavelength ranges corresponding to the plurality of LED lights, allowing the image capturing device 100 to selectively capture images that highlight particular colour changes relevant to the analyte under investigation. This selective imaging, coupled with the ability of each of the plurality of camera sensors to independently adjust exposure, focus, and resolution, enables the image capturing device 100 to capture even the most subtle colour variations with high fidelity. By collecting colour data in different colour spaces (e.g., RGB, HSV, or Lab), the plurality of camera sensors provides a comprehensive dataset for accurate quantification of analyte concentration, supporting a broad range of colorimetric assays.
[0045] Moreover, the use of the plurality of camera sensors allows the image capturing device 100 to create composite images by stitching data from each angle, resulting in a single, detailed view that enhances spatial resolution and minimizes artifacts. This composite imaging approach not only improves the visual quality of the captured image but also enables more precise measurement by averaging out any minor inconsistencies from individual sensor images. In kinetic assays or experiments requiring real-time data collection, the plurality of camera sensors can operate in synchronization with each other and with the plurality of LED lights, capturing images in rapid succession to monitor dynamic colour changes over time.
[0046] The plurality of camera sensors is integrated with the microcontroller of the image capturing device 100. The microcontroller implements advanced image processing techniques to analyse and compare colour intensities from the perspective of each of the plurality of camera sensors, thereby increasing measurement accuracy and repeatability. Overall, the plurality of camera sensors of the image capturing device 100 is fundamental to achieving high-resolution, reliable, and reproducible digital colorimetric analysis, making the image capturing device 100 ideal for applications where precise colour differentiation is essential for detecting and quantifying analytes in microfluidic samples.
[0047] In an embodiment of the present disclosure, the lower section 104 of the image capturing device 100 includes a microfluidic chip holder and a positioning slider 110. The microfluidic chip holder and the positioning slider 110 of the image capturing 100 are essential components that work together to ensure precise alignment, stability, and positioning of the microfluidic chip relative to the plurality of camera sensors and the plurality of LED lights. The microfluidic chip holder securely anchors the microfluidic chip in place, preventing any movement or shifting that could impact the consistency and accuracy of colorimetric measurements. Designed to accommodate various chip sizes and configurations, the microfluidic chip holder may include adjustable clamps or guides that allow for easy insertion and removal, enhancing versatility while safeguarding delicate microfluidic channels from damage.
[0048] Working in conjunction with the microfluidic chip holder, the positioning slider allows for fine adjustments along the X, Y, and sometimes Z axes, enabling precise control over the spatial placement of the microfluidic chip. This flexibility is crucial for achieving optimal alignment with the optical path of the plurality of camera sensors and ensuring uniform illumination across the sample area. By allowing the user to make subtle adjustments, the positioning slider ensures that each sample is centred within the field of view and that the focal plane aligns perfectly with the microfluidic channels, producing sharp and high-resolution images.
[0049] For colorimetric analysis, where even slight misalignment or focus errors can lead to inaccurate colour readings, the combined function of the microfluidic chip holder and positioning slider 110 is invaluable. The positioning slider enables precise focus control, ensuring that all parts of the sample are clearly visible and accurately represented in the captured images. In some advanced devices, the positioning slider may be motorized and programmable, allowing for automated positioning that ensures reproducible placement across multiple samples or repeated experiments, which is vital for consistency in quantitative analysis. Together, the microfluidic chip holder and the positioning slider 110 create a stable, adjustable platform that minimizes errors from misalignment, uneven lighting, or focus discrepancies. This stability and control are essential for achieving accurate, repeatable colorimetric measurements in microfluidic applications, ultimately contributing to the reliability and precision of the image capturing device for digital colorimetric analysis.
[0050] In an embodiment of the present disclosure, a microfluidic chip 112 with twenty-one microwells may be fitted into the microfluidic chip holder and the positioning slider 110 by a meticulous process that ensures each microwell is accurately aligned and properly illuminated for precise colorimetric readings. The microfluidic chip holder is designed with adjustable clamps or slots that securely accommodate the chip's dimensions, keeping it stable throughout the analysis process. Each microwell must be positioned to receive uniform lighting from the plurality of LED lights, which is essential for consistent colour representation across all microwells.
[0051] Once the microfluidic chip is placed in the microfluidic chip holder, the positioning slider enables fine adjustments along the X, Y, and Z axes to align the microwells perfectly within a field of view of the plurality of camera sensors. This control is particularly crucial for a multi-well microfluidic chip, as slight misalignment can lead to uneven illumination or partial occlusion of specific wells, which would compromise data accuracy. The positioning slider allows the user to centre the entire 21-microwell array, ensuring each well is fully visible and accessible for the camera sensors. Additionally, the Z-axis adjustment capability of the positioning slider helps achieve precise focus on the microwells, allowing the image capturing device 100 to produce sharp, high-resolution images of each well. This focus control is important, as it allows the image capturing device 100 to capture subtle colour variations in each microwell accurately. For applications that require analysis of all twenty-one microwells in a single run, the positioning slider ensures the microfluidic chip is positioned at an optimal distance from the plurality of camera sensors and plurality of LED lights, providing consistent measurements across the entire sample.
[0052] In an embodiment of the present disclosure, the image capturing device 100 is provided with an opening 114 for the positioning slider to provide a designated pathway for the positioning slider, allowing precise movement and adjustment of the microfluidic chip holder. The opening 114 enables the slider to move smoothly along the X, Y, and sometimes Z axes, ensuring that the microfluidic chip can be aligned accurately with the plurality of camera sensors and the plurality of LED lights. By providing a controlled space for the slider's motion, the opening 114 minimizes potential vibrations or shifts that could disturb the alignment, enhancing stability during image capture.
[0053] Additionally, the opening 114 keeps the movement of the positioning slider constrained to specific paths, allowing for repeatable, fine-tuned adjustments essential for achieving consistent positioning of the chip. This precision is crucial for obtaining reproducible results in colorimetric analysis, as it ensures that each sample is identically aligned and optimally illuminated. The opening 114 thus supports a stable, accurate, and user-friendly operation for high-quality colorimetric imaging.
[0054] In an embodiment of the present disclosure, the image capturing device 100 is provided with a passage 116 for electrical wires. The passage 116 of wires and wiring connections enable the integrated functioning of various electronic elements, such as the plurality of LED lights, the plurality of camera sensors, and control modules. The wiring connections supply power and transmit data between the components of the image capturing device 100, ensuring synchronized operation and precise control over lighting, imaging, and sensor adjustments. Organized within dedicated passages or channels, the wires are strategically routed to prevent tangling, interference, or physical strain, which could disrupt functionality or affect image quality. Efficient wire management within the image capturing device 100 helps maintain a clean internal structure, reducing electromagnetic interference (EMI) that could compromise the accuracy of colorimetric readings. Properly shielded wiring and secure connections are essential for preventing signal degradation, especially in systems that rely on high-speed data transfer for real-time image analysis. Additionally, organized wiring simplifies maintenance, as components can be easily accessed and repaired without disturbing the overall setup. Further, the wiring connections may include feedback loops and sensors to monitor voltage, current, or temperature, ensuring optimal performance and preventing overheating. This structured approach to wiring thus enhances the stability, reliability, and safety of the image capturing device 100, contributing to precise, repeatable colorimetric analysis within the microfluidic setup.
[0055] Fig. 2 illustrates an exemplary representation of a top view of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0056] Illustrated in Fig. 2 is a top view representation 200 of the image capturing device 100. The top cover 106 has been removed and the housing 108 of the upper section 102 is in full view. The housing 108 of the image capturing device 100 is a critical structural component that encloses and protects the plurality of LED lights, the plurality of camera sensors, the LED screen, the microcontroller, and the wiring connections. Designed for both durability and functionality, the housing 108 provides a stable, enclosed environment that shields sensitive internal components from dust, moisture, and physical impacts, which could otherwise degrade performance or accuracy over time. The housing 108 often has a sleek, compact design to allow for easy placement in laboratory environments, conserving bench space while offering robust protection.
[0057] Inside the housing 108, the layout is meticulously organized to ensure that each component, from the plurality of LED lights to the plurality of camera sensors, has dedicated space and remains securely fixed. This organized internal structure helps prevent vibrations or accidental displacements during operation, maintaining consistent alignment, especially crucial for the camera sensors and the plurality of LED lights. Moreover, the housing 108 plays a key role in minimizing light interference from external sources, creating a controlled illumination environment for accurate colorimetric measurements. The internal surfaces of the housing 108 are often coated or lined to further reduce light reflection and optimize the effectiveness, enhancing the precision of image capture. Thus, the housing 108 not only safeguards the internal components but also enhances operational efficiency and user safety by providing a stable, user-friendly structure that supports the high-performance requirements of the image capturing device 100 for precise digital colorimetric analysis.
[0058] Fig. 3 illustrates an exemplary representation of a top view of a lower section of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0059] Illustrated in Fig. 3 is a top view representation 300 of the lower section 104 of the image capturing device 100. The lower section 104 of the image capturing device 100 houses the microfluidic chip holder and the positioning slider 110. The microfluidic chip holder and the positioning slider 110 in the image capturing device 100 work together to secure and precisely align the microfluidic chip within the image capturing device 100. The microfluidic chip holder firmly grips the microfluidic chip, preventing any movement that could disrupt accurate measurements during imaging. Meanwhile, the positioning slider enables fine adjustments along the X, Y, and Z axes, allowing the microfluidic chip to be aligned perfectly with the plurality of camera sensors and the plurality of LED lights for uniform illumination and sharp focus. This precise positioning is essential for obtaining clear, reproducible images across all areas of the microfluidic chip, especially in multi-well setups where each well needs to be accurately centred. By ensuring stable placement and controlled alignment, the microfluidic chip holder and the positioning slider 110 enhance the accuracy and consistency of colorimetric analysis results. Together, the microfluidic chip holder and the positioning slider 110 provide a stable and adjustable platform, supporting reliable and high-quality imaging for sensitive assays in microfluidic applications.
[0060] Fig. 4A-4B illustrates exemplary representations of an upper surface and a lower surface of a microfluidic chip holder and a positioning slider of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0061] Illustrated in Figs. 4A-4B exemplary representations 400A-400B of an upper surface and a lower surface of the microfluidic chip holder and a positioning slider of the image capturing device 100. The upper surface of the microfluidic chip holder in the image capturing device 100 is typically designed to securely support and align the microfluidic chip. This surface often includes adjustable clamps or grooves that match the dimensions of the chip, holding the microfluidic chip firmly in place without damaging delicate microchannels. The upper surface may also have alignment markers or guides to ensure consistent positioning for repeated measurements. The lower surface of the microfluidic chip holder is connected to the positioning slider, which allows for precise movement along the X, Y, and sometimes Z axes. This lower surface is usually smooth or equipped with sliding tracks, enabling controlled, stable adjustments for aligning the chip with the plurality of camera sensors and the plurality of LED lights. The positioning slider may have a non-slip base or guide rails on its lower surface, providing stability and accuracy during fine adjustments, ensuring reliable alignment and focus for high-quality colorimetric imaging. The microfluidic chip is made up of glass with poly-vinyl chloride film stacked on top of each other. The image capturing device 100 may capture multiple images thus enabling colorimetric detetcion for multiple analytes at a time. Accordingly, the microfluidic chip is design to have twenty-one distinct microwells, each well capable of analyte concentration detection colorimetrically.
[0062] Fig. 5 illustrates an exemplary representation of a battery casing and cover plate of the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0063] Illustrated in Fig. 5 is an exemplary representation of the battery casing 500 and the cover plate of the image capturing device 100. The battery casing 500 may be positioned at the base of the image capturing device 100. The image capturing device 100 is equipped with the battery casing 500 and cover plate to enhance its functionality, portability, and safety. The battery casing 500 serves as a protective enclosure for the power source of the image capturing device 100, typically housing rechargeable batteries that ensure uninterrupted operation even in the absence of a direct power supply. This enables the image capturing device 100 to be used in various settings, including remote laboratories or field applications, where access to electrical outlets may be limited. Additionally, the battery casing 500 is designed to safeguard the batteries from physical damage, environmental factors such as moisture and dust, and potential short circuits, thereby extending the overall lifespan of the power components.
[0064] The cover plate plays a crucial role in protecting the internal electronics and sensitive components, including the plurality of LED lights, the plurality of camera sensors, the microcontroller, and the wiring connections, from external contaminants and accidental damage. The cover plate ensures that the image capturing device 100 remains sealed against dust, spills, and other environmental hazards that could compromise performance or accuracy. The cover plate also contributes to the structural integrity of the image capturing device 100, providing a stable and secure enclosure that maintains the precise alignment of internal components essential for accurate colorimetric analysis. Furthermore, the cover plate often incorporates access points or compartments for easy battery replacement or maintenance, allowing users to service the image capturing device 100 without exposing sensitive parts. The cover plate may also include ventilation features or thermal management components to prevent overheating, especially when the image capturing device 100 operates for extended periods or under high-intensity lighting conditions. Further, the cover plate and battery casing 500 contribute to the professional appearance, making it suitable for use in clinical, research, and industrial environments. The battery casing 500 and the cover plate also enhance user safety by enclosing all electrical components, reducing the risk of electrical shocks or accidental contact with moving parts. Additionally, the cover plate may be designed with user-friendly features such as labelling, indicators for battery status, and secure locking mechanisms to ensure that the image capturing device 100 remains properly closed during operation. The inclusion of the battery casing 500 and cover plate ensures that the image capturing device 100 is robust, reliable, and versatile, capable of delivering high-precision colorimetric analysis while being protected against various operational and environmental challenges. These components are essential for maintaining the performance, durability, and ease of use of the image capturing device 100, ultimately supporting consistent and accurate analytical results in microfluidic applications.
[0065] Fig. 6 illustrates an exemplary flow representation of a method of using the proposed image capturing device for digital colorimetric analysis in a microfluidic device, in accordance with an embodiment of the present disclosure.
[0066] Illustrated in Fig. 6 is a flow diagram representation of the method 600 of using the image capturing device 100. The method 600 begins with obtaining 602, by the microcontroller, the images of the samples from the plurality of camera sensors. The method 600 proceeds with processing 604 the images, by the microcontroller, for extraction of data. The method 600 proceeds with performing 606, by the microcontroller, digital colorimetric detection of biomolecules on the processed images. The method 600 ends with obtaining 608, by the microcontroller, corresponding concentration values based on the digital colorimetric detection.
[0067] While the foregoing describes various embodiments of the invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions, or examples, which are comprised to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
, Claims:1. An image capturing device (100) for digital colorimetric analysis in a microfluidic device, the image capturing device (100) comprising:
an upper section (102) comprising a housing (108) for enclosing a plurality of LED lights configured to provide uniform illumination across microfluidic samples enabling precise quantification of colour changes related to analyte concentration, a LED screen configured to display real-time images of the microfluidic samples, a plurality of camera sensors configured to capture images of the microfluidic samples from multiple angles, and a microcontroller, operatively coupled with the plurality of camera sensors and configured to:
obtain the images of the microfluidic samples from the plurality of camera sensors;
process the images for extraction of data;
perform digital colorimetric detection of biomolecules on the processed images; and
obtain corresponding concentration values based on the digital colorimetric detection,
and
a lower section (104) comprising a microfluidic chip holder configured to securely position a microfluidic chip (112) ensuring precise alignment and stable illumination for accurate colorimetric analysis and a positioning slider (110) configured to enable fine adjustments to positioning of the microfluidic chip (112) ensuring optimal focus and alignment for accurate colorimetric measurements,
wherein the image capturing device (100) is configured to capture a plurality of images thus enabling simultaneous colorimetric detection for a plurality of analytes.
2. The image capturing device (100) as claimed in claim 1, wherein the plurality of LED lights is configured to provide consistent and controllable illumination across the microfluidic chip (112) allowing even light distribution and minimizing shadows or glare that could distort colour readings.
3. The image capturing device (100) as claimed in claim 1, wherein the LED screen is configured to overlay analytical data directly on the captured images which enhances accuracy of colorimetric readings and enables real-time assessment of quantitative results.
4. The image capturing device (100) as claimed in claim 1, wherein the plurality of camera sensors is optimized to capture specific colour wavelengths corresponding to LED illumination, ensuring that even subtle colour variations are recorded accurately.
5. The image capturing device (100) as claimed in claim 1, wherein the microcontroller is configured to apply deep learning techniques to process the images captured by the plurality of camera sensors.
6. The image capturing device (100) as claimed in claim 1, wherein the microfluidic chip holder is compatible with a glass microfluidic device with a multi-layered polyvinyl chloride film.
7. The image capturing device (100) as claimed in claim 1, wherein the microfluidic chip holder is configured to lock the microfluidic chip (112) into a specific plane relative to the plurality of camera sensors and the plurality of LED lights, ensuring that the microfluidic chip (112) remains flat and parallel to the plurality of LED lights, which is essential for achieving even illumination and accurate imaging across a sample area.
8. The image capturing device (100) as claimed in claim 1, wherein the microfluidic chip holder is configured to work in conjunction with the positioning slider (110), enabling fine-tuned adjustments in all three dimensions (X, Y, and Z axes) to achieve optimal focus and alignment with image capturing optics.
9. The image capturing device (100) as claimed in claim 1, wherein the microcontroller is powered by an AC adapter and a battery pack.
10. A method (600) of operating an image capturing device (100) for digital colorimetric analysis in a microfluidic device, the method (600) comprising steps of:
obtaining (602), by a microcontroller, images of microfluidic samples from a plurality of camera sensors;
processing (604), by the microcontroller, the images for extraction of data;
performing (606), by the microcontroller, digital colorimetric detection of biomolecules on the processed images; and
obtaining (608), by the microcontroller, corresponding concentration values based on the digital colorimetric detection.

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