Highly sensitive glucose sensor based on MnFe2O4@SiO2/Nafion modified glassy carbon electrode

Abstract

The facile sonochemical method was employed to synthesize MnFe2O4@SiO2 nanocomposite and was well-characterized using spectral, analytical, electrochemical, and theoretical studies. The as-synthesized MnFe2O4@SiO2 nanocomposite material showed excellent properties owing to its surface area, catalytic property, stability, reproducibility, and sensitivity towards biomolecules like D-Glucose. The electron transfer resistance value was estimated to be less compared to MnFe2O4 and SiO2 nanomaterials. Fabricated MnFe2O4@SiO2 immobilized glassy carbon electrode (GCE) sensor exhibited higher sensitivity and low detection limit (0.0023 µM) against D-glucose molecule in phosphate buffer medium at pH-7.0 than other reported electrodes in the literature. The MnFe2O4@SiO2/Nafion/GCE modified sensor was used for real blood sample analysis and its recovery results were found to be good.

Specification

Description:FIELD OF INVENTION
[001] The field of invention in patent applications refers to biosensing nanotechnology. In this, a developed MnFe2O4@SiO2/Nafion/GCE modified sensor is utilized to D-glucose molecule in human blood samples by electrochemical technique.
BACKGROUND AND PRIOR ART
[002] Determination and monitoring of glucose level in blood is receiving much attention for human beings affected by diabetes, High level of blood glucose level leads adverse effect to the human health such as heart attack, kidney problem, eye sight issues and age-related diseases. Many spectral, analytical methods are availed to monitor the glucose level now days, among them electrochemical sensor based techniques are important in terms of fast response, easy operation, accuracy, low cost, low detection limit, portability, high sensitivity and stability of the electrode material etc. Till date, no work was reported using MnFe2O4@SiO2/Nafion/GCE modified sensor for D-glucose sensing. This is the first work we have developed MnFe2O4@SiO2/Nafion/GCE modified sensor for highly sensitive and low detection limit (LOD: 0.0023 µM) detection of glucose level in human blood samples by cyclic voltammetric technique at pH-7.0. The evaluated values of detection limit, wide linear range of response, and sensitivity are found to be better as compared to the reported electrodes for D-glucose.
SUMMARY OF THE INVENTION
[003] MnFe2O4@SiO2 nanocomposite material was prepared using the sonochemical method and calcined by combustion technique and the as-prepared material was analyzed by UV-vis, FT-IR, SEM, EDX, HR-TEM and SEAD, and electrochemical techniques. The developed MnFe2O4@SiO2/Nafion/GCE modified sensor was used for the investigation of the accurate detection of D-glucose in human blood samples and it is monitored by electrochemical techniques. The detection limit was found to be low (0.0023 µM), higher sensitivity, and linear range of response are achieved for the detection of D-glucose at pH-7.0. The present modified sensor may be used for clinical, forensic, and diagnostic applications in future under optimized environment conditions.
BRIEF DESCRIPTIONS OF DRAWINGS
[004] The drawing descriptions are following the steps, MnFe2O4@SiO2 nanocomposite material was prepared by sonochemical method. The result product was filtered using conical flask using stem-cut funnel with Whatman no.1 filter paper. The as-prepared MnFe2O4@SiO2 nanocomposite material was calcined using solution combustion method in a muffle furnace at 450 ºC. The ash-like powdered MnFe2O4@SiO2 nanocomposite material was dispersed in 5 ml ethanol containing 5 µM solution of Nafion polymer and sonicated for 30 mins. Coating of MnFe2O4@SiO2/Nafion on the polished GCE surface (0.5 mm diameter) using micro-syringe by drop casting technique and dried at room temperature. The developed MnFe2O4@SiO2/Nafion immobilized GCE used as a working electrode for the electrochemical sensing of biomolecules.
DETAILED DESCRIPTION OF THE INVENTION
[005] The electrochemical methods such as CV and DPV were used in this study. To setup electrochemical cell, MnFe2O4@SiO2 used as an electrode material, nafion polymer used as an immobilizer on the glassy carbon electrode (GCE), Phosphate buffer saline (PBS) (0.1 M) was utilized as electrolyte in this study. A conventional three electrode systems were employed such as MnFe2O4@SiO2/Nafion immobilized GCE as a working electrode, Ag/AgCl filled with saturated KCl as a reference electrode, and platinum was used as a counter electrode. A wide linear range of response was found when increasing the concentration of D-glucose in human blood samples with good correlation coefficient (R2) value. The limit of detection was calculated to be 0.0023 µM and the sensitivity was estimated to be 0.0242 µAµM/cm2 respectively. The selectivity of the D-glucose was analyzed with 100-fold excess presence of the interferents' molecules such as ascorbic acid, glutamic acid, methionine, cysteine, alanine, lecuine, and serine biomolecules. , C , Claims:1. This work claims the fabrication of MnFe2O4@SiO2/Nafion immobilized GCE electrode and it's the selective sensing applications towards D-glucose in human blood samples in 0.1 M PBS as electrolyte.

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