Formulation of agrowaste and essential ingredients based antimicrobial diffuser oil

Abstract

This invention relates to a formulation of antimicrobial diffuser oil and in particular, this invention relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil. This invention also relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which contains Loban, Citrus limmeta and other herbal ingredients. More particularly, this present invention relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which exhibits excellent antibacterial and antifungal activity through direct effect on harmful microorganisms, thereby being able to be usefully used for a pharmaceutical composition, a cosmetic composition, a preservative for food or feed preservation, etc.

Specification

Description:Field of the Invention

[001] This invention relates to a formulation of antimicrobial diffuser oil and in particular, this invention relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil. This invention also relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which contains Loban, Citrus limmeta and other herbal ingredients. More particularly, this present invention relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which exhibits excellent antibacterial and antifungal activity through direct effect on harmful microorganisms, which causes common infections. thereby being able to be usefully used for a pharmaceutical composition, a cosmetic composition, a preservative for food or feed preservation, etc.

Background of the invention and the related Prior Art

[002] A myriad of microorganisms inhabit the skin and cause various skin diseases. Examples of such microorganisms include Staphylococcus aureus causing skin suppuration and resident skin flora such as Staphylococcus epidermis, Propioni- bacterium acnes and Pityrosporum ovale. These bacteria decompose sebaceous matter and sweat secreted from the skin, thus causing unpleasant odors and the resulting decomposed products irritate the skin, causing inflammation. In particular, Propionibacterium acnes decomposes sebum to produce free fatty acids, utilizing lipase which is a fatty acid-decomposing enzyme. The resulting free fatty acids not only irritate skin, but also cause inflammatory lesions such as red rashes including papules, pustules and nodules, as exhibited in acne. Dermatophytes invade the stratum corneum of hairs and skin, causing superficial mycoses. As examples of Dermatophytes causing superficial mycoses, mention may be made of Microsporum, Trichophyton and Epodermophyton which are pathogens causing trichophytosis. Candida albicans causes skin candidiasis and chronic skin/mucosal candidiasis. Such fungi cause superficial mycoses leading to various lesions due to their parasitic nature and proliferation on corneous tissues of the skin. As a causative agent that causes granulomatous lesions on other skin and subcutaneous tissues, mention may be made of the pathogenic fungi Sporothrix schenckii.

[003] Meanwhile, among herbal medicines, there are known a large number of medicines that have been widely utilized as external preparations since ancient times, in treatment of skin diseases caused by bacteria and fungi. Recently, a great deal of attempts have been made to scientifically elucidate effectiveness of herbal medicines that have been conventionally used in folk remedies and passed on from generation to generation, and based on results therefrom, research to find active ingredients of herbal medicines having novel antimicrobial activity is being actively undertaken. As a relatively well-known herbal medicine having antimicrobial activity, berberine, which is an alkaloid contained in Phellodendron amurense, Coptis japonica, etc., is listed as berberine chloride in the Korean Pharmacopoeia and has a broad antimicrobial spectrum.
[004] The document US2023398051 relates to a skin-care composition comprising beta-glucan and a natural oil, as well as either one or both of hydrogen peroxide and lactic acid. The skin-care composition is mainly based on natural ingredients. The composition has biocidal/anti-microbial activity and is very skin-friendly and non-irritative. The skin-care composition contains beta-glucan, which has an emulsifying effect, as well as a smoothening and moisturizing effects on the skin. A skin-care composition of the present invention does not negatively affect the barrier function of the skin.
[005] The cited document CN107823602 discloses an anti-microbial microcapsule of Chinese herbal medicine essential oil and its preparation method. The anti-microbial microcapsule is prepared by mixing wasabi essential oil,folium artemisiae argyi essential oil, coral ginger essential oil, and cinnamon essential oil as a capsule core, taking chitosan, sodium caseinate, konjac glucomannan, and carboxymethylcellulose as acapsule wall material, and applying sucrose ester as the emulsifier. By adopting the microcapsule technology, the Chinese herbal medicine essential oil with bacteria resistance and bacteriostasis effects can be embedded; the Chinese herbal medicine essential oil with different with bacteria resistance and bacteriostasis effects is reasonably mixed, thus the microcapsule has broad-spectrum bacteriaresistance and bacteriostasis effects, obvious anti-microbial and itching relieving effects, good stability and long efficiency, long acting time of bacteria resistance and bacteriostasis, and smalltoxic and side effects.
[006] The document CN105968425 belongs to the field of food antimicrobial preservation, in particular to a preparation method of an edible anti-microbial composite membrane containing tea tree oil three-level nano-liposomes, which is auxiliarily processed by a plasma. The preparation method comprises the following steps of dissolving tara gum into a certain amount of deionized water; water bathing at the temperature of 80 DEG C and stirring for 30min; then dissolving low-methoxy pectin into the deionized water; respectively adding CaCl2, Nisin and citric acid; water bathing and stirring uniformly; then adding glycerinum to form gel, and cooling to be the room temperature; ultrasonically removing gas in a membrane dispersion system; pouring into a vessel; drying for 12h in a constant temperature and humidity drying oven at the temperature of 25 DEG C; processing the composite membrane for 1 to 3min by the 50 to 100W cold plasma; after a surface is rough, spraying the prepared tea tree oil three-level nano-liposomes; continuously drying to form the membrane. The preparation method of the edible anti-microbial composite membrane containing the tea tree oil three-level nano-liposomes, which is auxiliarily processed by the plasma, can be applied in the packaging of fresh food, and is beneficial to effectively prolonging the expiration date of the fresh food.
[007] The patent document KR102171948 relates to an antibacterial and antifungal composition containing a mixed herbal essence of Origanum majorana, Cymbopogon citratus, Aniba rosaeodora, Cymbopogon nardus, Melaleuca alternifolia, and Thymus vulgaris as an active component. The mixed herbal essence of Origanum majorana, Cymbopogon citratus, Aniba rosaeodora, Cymbopogon nardus, Melaleuca alternifolia, and Thymus vulgaris.
[008] WO1999027793 disclosed an antimicrobial composition comprising an antimicrobially effective amount of clove bud oil and two or more of eucalyptus oil, cajaput oil, lemongrass, lavender or tea tree oils. Use of the composition as a treatment for cold sores, head lice, vaginal thrush, verruca, warts, athletes foot, an antimicrobial mouth wash in addition to a surface cleaner are also disclosed.
[009] The document US6846498 discloses an antimicrobial composition and more particularly a germicidal spray for sanitizing and disinfecting surfaces including leather, wood, metal, plastic and fabric, comprises a mixture of essential oils capable of being dissolved or dispersed by a solvent in a water carrier, oils and an ionizing agent such as Blue Stone ions which speeds up the antimicrobial activity of the essential oils. The product of the invention is completely natural and non-toxic to adults and children even if swallowed.
[010] US-8741271 describes compositions for cleaning, disinfecting and sanitizing are described. The compositions comprise essential oils and are stable, non-toxic and environmentally sustainable. Also disclosed are deodorants and scalp refreshers having these properties.
[011] Monika Sienkiewicz, et al (2012) describes essential oils derived from aromatic plants possess useful properties concerning human health, such as antibacterial, antiviral and antifungal activities. They can be used as natural alternatives to synthetic preparations to prevent and treat infectious diseases caused by multidrug resistant microorganisms. They are used successfully to treat respiratory tract, digestive system and skin infections. Many of them may be applied in anticancer therapy, cardiovascular and nervous system disorders, as well as to reduce the level of cholesterol and decrease and regulate the glucose level. Due to their ability to stimulate adrenal and estrogen hormone production and their antimicrobial properties, they are useful in the treatment of gynecological diseases. Oils are commonly used in the food and cosmetic industry. The present paper describes recent patents concerning potential uses of essential oils in human health and treatment of diseases.

[012] The invention consists of certain novel features and a combination of ingredients hereinafter fully described, and particularly pointed out in the appended claims, it being understood that various changes in the details may be made without departing from the spirit, or sacrificing any of the advantages of the present invention.

Summary of the invention
[013] This invention relates to a formulation of antimicrobial diffuser oil and in particular, this invention relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil. This invention also relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which contains Loban, Citrus limmeta and other herbal ingredients. More particularly, this present invention relates to the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which exhibits excellent antibacterial and antifungal activity through direct effect on harmful microorganisms, thereby being able to be usefully used for a pharmaceutical composition, a cosmetic composition, a preservative for food or feed preservation, etc.

Detailed description of the invention with accompanying drawings
[014] For the purpose of facilitating an understanding of the invention, there is illustrated in the preferred embodiment along with accompanying drawings thereof, from an inspection of which, when considered in connection with the following description along with accompanying drawings, the invention, its construction and operation, and many of its advantages should be readily understood and appreciated.

[015] The principal object of the invention is to provide formulation of agro waste and essential ingredients based antimicrobial diffuser oil.
[016] The other object of the invention is to provide the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which comprises the ingredients Loban; Citrus limmeta; Citrus sinensis ; Lemon grass oil ; Mentha and Deorderized Kerosene in particular ratio.
[017] The other object of the invention is to provide the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which comprises;
- 5 gm of Loban;
-20ml of Citrus limmeta ;
-20ml of Citrus sinensis ;
-5ml of Lemon grass oil ;
-1ml of Mentha and
-50ml of Deorderized Kerosene;
[018] The other object of the invention is to provide the process of preparing the formulation of agro waste and essential ingredients based antimicrobial diffuser oil as claimed in claim 1 which comprises the ingredients Loban; Citrus limmeta; Citrus sinensis ; Lemon grass oil ; Mentha and Deorderized Kerosene in particular ratio.

[019] The other object of the invention is to provide the process of preparing the formulation of agro waste and essential ingredients based antimicrobial diffuser oil which comprises the mixing the ingredients of a) 5 gm of Loban; b) 20ml of Citrus limmeta ;c) 20ml of Citrus sinensis ; d) 5ml of Lemon grass oil ; f) 1ml of Mentha and g) 50ml of Deorderized Kerosene homogeneously and prepare the mixture product.

[020] The novel features that are considered characteristic of the present invention are set forth with particularity in the appended claims. The invention itself, however, both as to its composition and its process of preparation, together with additional objects and advantages thereof, will best be understood from the following description of certain specific embodiments, when read in connection with the accompanying drawings, in which:

[021] Fig 1- illustrates the Total number of colonies isolated from air :72 Total number of colonies isolated after using diffuser of the present invention;
[022] Fig 2- illustrates the Antimicrobial activity of Mentha oil and loban against Staphylococcus aureus Antimicrobial activityof the present invention;
[023] Fig 3- illustrates the Antimicrobial activity of Mentha oil and loban against E.coli of the present invention;

[024] In todays life , due to lack of spare time, people often seek short term relief measures in the form of allopathy, ie, conventional means of consumption of drugs that incite symptoms contrasting to those of the disease. In accordance to the present world, the demographic scenario has been affected, witnessing the enhanced rate of diseases due to the rampant handling of chemicals. Fumigation has been used effectively as a process of decontamination after bio terrorism events in agricultural as well as residential structures. However, even in these situations, life threatening incidents may occur by the accidental breakout of large amount of fumigants cause OMG serious havoc to the exposed workers as well as the public. Witnessing the most fatal ordeal of today, the research project brings attention to the extraction of natural plant extracts for combatting the problem by unique sterilisation technique through the development of Sanjeevani- the herbal antimicrobial diffuser.Methicillin resistant Staphylococcus aureus MRSA and other gram positive bacteria has become an increasingly common problem problem in health care sectors. The project being a herbal plant based sterilising system will prove to be a great help as well as a nobel procedure for the elimination of the various airborne microbes present in the environment. The product basically deals with the creation of tremendously efficacious neotechnology i.e., our green peculiar approach named Sanjeevani- A great antimicrobial herbal diffuser/vaporizer.
[025] Antimicrobial activity has been studied against staphylococcus aureusand zone of inhibition has been formed with the component of the herbal constituents have been formulated in a proportion of
Loban :5g
Citrus limmeta-20ml
Citrus sinensis- 20ml
Lemon grass oil: 5ml
Mentha : 1ml
Deorderized Kerosene: 50ml
[026] Different combinations have been tried which are illustrated by the following examples. Out of five examples given below, example V is found to be most suitable because it obviates the drawbacks.
[027] Example I :
All the ingredients of 6 gm of Loban; b) 50ml of Citrus limmeta ;c) 20ml of Citrus sinensis ; d) 5ml of Lemon grass oil ; f) 2 ml of Mentha and g) 50ml of Deorderized Kerosene homogeneously and prepare the mixture product.
[028] Example II :
All the ingredients of 3 gm of Loban; b) 40ml of Citrus limmeta ;c) 50ml of Citrus sinensis ; d) 7 ml of Lemon grass oil ; f) 2 ml of Mentha and g) 70ml of Deorderized Kerosene homogeneously and prepare the mixture product.
[029] Example III :
All the ingredients of 4 gm of Loban; b) 60ml of Citrus limmeta ;c) 50ml of Citrus sinensis ; d) 12 ml of Lemon grass oil ; f) 6ml of Mentha and g) 40ml of Deorderized Kerosene homogeneously and prepare the mixture product
[030] Example IV :
All the ingredients of 6 gm of Loban; b) 60ml of Citrus limmeta ;c) 50ml of Citrus sinensis ; d) 8ml of Lemon grass oil ; f) 9ml of Mentha and g) 60ml of Deorderized Kerosene homogeneously and prepare the mixture product.
[031] Example V :
All the ingredients of 5 gm of Loban; b) 20ml of Citrus limmeta ;c) 20ml of Citrus sinensis ; d) 5ml of Lemon grass oil ; f) 1ml of Mentha and g) 50ml of Deorderized Kerosene homogeneously and prepare the mixture product.

[032] The results of this study showed that this particular novel combination herbal product as stated in example V can make a significant difference.
[033] The efficacy of the present invention lies in the formulation of the composition of the ingredients in a particular ratio which is highly significant as shown.

[034] In accordance to the present world, the demographic scenario has been affected due to the rampant handling and inhaling chemical.An herbal fumigator is a need of hour because in these situations, there have been incidents where chemical fumigants have developed resistance to most of the pathogenic microorganism. In this era of globalization industrialization and urbanization this product is sustainable meaning no harm to mother nature and Human Host.
[035] LOBAN : Extracted from the fragrant balsamic resin obtained from the bark of a tree under the genus Styrax., It gives an anxiolytic effect, Serves as an excellent insect repellent and fumigant.
[036] Mentha: Use medicinally. Can be also be used as decongestant, promote easier breathing, temporarily relieve nasal congestion, soothe sore throats, headaches, and sinus discomforts, support immunity, and stabilizes the emotions. Lemon Grass:Lemongrass essential oil is used in aromatherapy to freshen the air, reduce stress, and uplift the mood. Lemongrass is also used as a folk remedy to promote sleep, relieve pain, and boost immunity.
[037] Citrus Limmetta (Sweet Lime) The fruit that we typically associate with limes is not the same as sweet lime. The fruit resembles a giant lemon in size when fully ripe, and its skin is either pale green or, more frequently, yellow. The tartness-causing acidity is absent from the flavor. The end product has a light, crisp flavor that is best consumed straight from the tree or as a juice. This unusual citrus has dense, evergreen foliage that is frequently distinctly cupped or rolled. It is said to be a cross between a Mexican lime and a sweet lemon.
[038] Sweet lime, like all citrus fruits, contains potent vitamins and nutrients. Its high vitamin C and folic acid content ensure strong immunity, glowing skin health, and support for bones and joints. As explained in the book "Health-Promoting Properties of Fruits and Vegetables" (Simonne et al., 2011), citrus also contains other extremely beneficial compounds including chlorophyll, carotenoids, phenolics, flavonoids, and limonoids. According to the "Nutritive Value of Indian Foods" published by the National Institute of Nutrition, 100g of sweet lime has following significant nutrient values: Sweet limes are used for many purposes. For example, they are suitable for eating and contain oils, including peel oil as well as juice oil, which are volatile in nature. They contain a high amount of ascorbic acid (Sandhu & Minhas, 2006), and their juice is used as a sweet drink in summer. Limonene (60.17%) is the most abundant terpene, followed by gamma-terpinene (11.80%), terpinolene (2.96%), beta-pinene (2.08%), linalool (1.85%), p-cymene (1.38%), geranial (1.33%), and alpha-terpineol (1.28%). The remaining terpenes amount to less than 1% and are found in the peel oil of the sweet lime (Abdul et al., 1992).
[039] ORANGE (CITRUS SINENSIS)
[040] This family includes the sweet orange (Citrus sinensis L. Osbeck), sometimes known as orange. It is a key source of vitamins, particularly vitamin C, and has adequate amounts of folacin, calcium, potassium, thiamine, niacin, and magnesium (Angew, 2007). Oranges are a valuable fruit crop from an economic standpoint; as of 2005, production of 60 million metrictonnes of oranges was valued at $9 billion globally. Half of this total originated in the United States and Brazil (Goudeau et al., 2008; Bernardi et al., 2010). According to FAO estimates from 2009, there were nine million hectares of citrus growing worldwide, and 122.3 million tons of fruit were produced, placing sweet oranges as the most popular fruit crop (Xu et al, 2013).(6) Citrus sinensis (L. Osbeck) or sweet orange originated from south East Asia, but is consumed all over the world as an excellent source of vitamin C, a powerful natural antioxidant that builds the body immune system. Important phytochemicals like liminoids, synephrine, hesperidin flavonoid, polyphenols, pectin, and sufficient amount of folacin, calcium, potassium, thiamine, niacin and magnesium are also present. These biologically active compounds prevent arteriosclerosis, cancer, kidney stones, stomach ulcers and reduction in cholesterol level and high blood which promote human health(6).
[041] NUTRITIVE VALUES
[042] Citrus sinensis (L. Osbeck) or sweet orange originated from south East Asia, but is consumed all over the world as an excellent source of vitamin C, a powerful natural antioxidant that builds the body immune system. Important phytochemicals like liminoids, synephrine, hesperidin flavonoid, polyphenols, pectin, and sufficient amount of folacin, calcium, potassium, thiamine, niacin and magnesium are also present.

[043] In the preceding specification, the invention has been described with reference to specific exemplary embodiments thereof. It will be evident that various modifications and changes may be made thereunto without departing from the broader spirit and scope of the invention as set forth in the claims that follow. The specification and drawings are accordingly to be regarded in an illustrative rather than restrictive sense. Therefore, the aim in the appended claims is to cover all such changes and modifications as fall within the true spirit and scope of the invention. The matter set forth in the foregoing description and accompanying drawings is offered by way of illustration only and not as a limitation. The actual scope of the invention is intended to be defined in the following claims when viewed in their proper perspective based on the prior art.





, Claims:We claim:

1) A formulation of agro waste and essential ingredients based antimicrobial diffuser oil which comprises the ingredients Loban; Citrus limmeta; Citrus sinensis ; Lemon grass oil ; Mentha and Deorderized Kerosene in particular ratio.
2) The formulation of agro waste and essential ingredients based antimicrobial diffuser oil as claimed in claim 1 which comprises;
- 5 gm of Loban;
-20ml of Citrus limmeta ;
-20ml of Citrus sinensis ;
-5ml of Lemon grass oil ;
-1ml of Mentha and
-50ml of Deorderized Kerosene;
3) A process of preparing the formulation of agro waste and essential ingredients based antimicrobial diffuser oil as claimed in claim 1 which comprises the ingredients Loban; Citrus limmeta; Citrus sinensis ; Lemon grass oil ; Mentha and Deorderized Kerosene in particular ratio.
4) The process of preparing the formulation of agro waste and essential ingredients based antimicrobial diffuser oil as claimed in claim 1 which comprises the mixing the ingredients of a) 5 gm of Loban; b) 20ml of Citrus limmeta ;c) 20ml of Citrus sinensis ; d) 5ml of Lemon grass oil ; f) 1ml of Mentha and g) 50ml of Deorderized Kerosene homogeneously and prepare the mixture product.

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