DEVELOPMENT OF FIBRIN BIOMATERIAL FOR WOUND HEALING AND BLOOD CLOT

Abstract

FORM 2 ABSTRACT Fibrin-based biomaterials are rapidly advancing as a cutting-edge solution in wound healing and hemostasis. Leveraging fibrin, a natural protein central to the body’s clotting process, these biomaterials create a supportive network that not only stabilizes clots but also fosters cellular migration and tissue repair. Derived from human or animal fibrinogen and thrombin, fibrin biomaterials are often strengthened with crosslinking agents to boost their durability. Engineered fibrin scaffolds can incorporate growth factors, cells, and bioactive compounds, enhancing their regenerative capabilities and making them versatile tools for surgical hemostasis, chronic wound care, tissue engineering, and targeted drug delivery. While fibrin biomaterials offer significant benefits, including biodegradability and biocompatibility, challenges like mechanical fragility, immune responses, and controlled degradation remain. To overcome these, recent innovations focus on hybrid materials and nanotechnology, aiming to unlock the full potential of fibrin biomaterials in wound care and regenerative medicine. Fibrin, a protein formed during the blood clotting cascade serves as a scaffold that facilitates the repair of damaged tissue. Fibrin is formed from fibrinogen when the enzyme thrombin is activated. Fibrin fibers from a mesh like structure that traps red blood cells and platelets stabilizing the blood clot. Fibrin forms the temporary matrix for cell attachment, migration and tissue repair, it supports angiogenesis (formation of New blood vessels) and the migration of fibroblasts and keratinocytes, key cells in tissues repair KEYWORDS: Wound Healing ,Blood Clotting, Fibrinogen, Thrombin, Fibrin Biomaterial.

Specification

FORM 2
THE PATENT ACT 1970
(39 of 1970)
The Patents Rules, 2003 /COMPLETE
SPECIFICATION
(See section 10 and njt*13)

1. 1. TITLE OF THE INVENTION: DEVELOPMENT OF FIBRIN BIOMATERIAL FOR
WOUND HEALING AND BLOOD CLOT
2. APPLICANT (S)
(a) NAME: Ms. S. PAVITHRA
(b) NATIONALITY: Indian
(c) ADDRESS: Sri gj^^lnstitute of Engineering and Technology, L&T By-Pass Road,
Coimbatore, Tamil Nadu, 641062
3. PREAMBLE TO THE DESCRIPTION
4. DESCRIPTION (Description shall start from next page.)

5. CLAIMS (not applicable for provisional specification. Claims should start with the preamble -
"I/we claim" on separate page)
6. DATE AND SIGNATURE (to be given at the end of last page of specification)
7. ABSTRACT OF TNE INVENTION (to be given along with complete specification on separate page)

Note: -
* Repeat boxes in case of more than one entry.
uTo be signed by the applicant(s) or by registered patent agent. Name of the applicant should be given in full, family name in the beginning.
* Complete address of the applicant should be given stating the postal index no./code, state and
country. |
* Strike out the column which Is/are not applicable

FORM 2
PREAMBLE TO THE DESCRIPTION
1. Today's world in biomaterials science are paving the way for innovative treatments in wound healing and hemostasis, with fibrin-based biomaterials at the forefront. Fibrin, a key protein in the body's natural clotting and repair
treatment, offers remarkable potential as a foundation for biomaterials that support faster healing, effective clot formation, and reduced complications.

2. By mimicking the body's natural mechanisms, fibrin biomaterials provide a biocompatible scaffold for cell growth, tissue regeneration, and sustained hemostasis, making them promising candidates for various medical applications, from surgical wounds to trauma care.

3. This exploration will examine the development and application of fibrin biomaterials, focusing on their structure, functionality, and impact on the future of wound care and regenerative medicine. Through understanding fibrin's role
in wound healing and blood clotting, we can better appreciate the transformative possibilities that these biomaterials bring to modern medicine.

DESCRIPTION
1.
Wound healing is a complex process that involves the body's natural to tissue injury and its refers to a living organisms replacement of destroyed or damaged tissue by newly produced tissue. In undamaged skin, the epidermis and dermis form a protective barrier against the external environment and also blood clot is a vital process that prevents excessive bleeding when a blood vessel is injured. It occurs when platelets and proteins in the blood work together to form a clot over the damaged area. The body usually dissolves the clot once the injury has healed.

2. Fibrinogen and thrombin are proteins that play important roles in blood clotting and hemostatic a proteins produced in the liver that circulates in the blood plasma. Fibrinogen is a risk factor for thrombosis and is involved in a complex interplay with thrombin and fibrin of protein produced during coagulation that converts fibrinogen into fibrin which forms blood clot to stop bleeding.

3. The development of biomaterial fibrin, a natural biopolymer, plays a crucial role in wound healing and in addition to its important role in hemostasis due to their ability to mimic the natural coagulation process and promote tissue regeneration.

4. Fibrin, a protein formed during the blood clotting cascade serves as a scaffold that facilitates the repair of damaged tissue. Fibrin is formed from fibrinogen when the enzyme thrombin is activated. Fibrin fibers from a mesh like structure that traps red blood cells and platelets stabilizing the blood clot. Fibrin forms the temporary matrix for cell attachment, migration and tissue repair, it supports angiogenesis (formation of New blood vessels) and the migration of fibroblasts and keratinocytes, key cells in tissues repair

PROBLEM DESCRIPTION:
1. In case of severe wounds, chronic wound, or patient with clotting disorder so, we are developing a fibrin biomaterial for natural wound healing

2. Delayed re-epithelization it's provides stability for a growth factor for natural tissue repair

3. Ensuring the fibrin biomaterial is biocompatible and reduces risks of immune reactions, inflammation, particularly for patients with sensitive or compromised immune systems

The objectives of this invention are,
1. Develop a fibrin biomaterial that accelerates wound healing by providing a scaffold for tissue regeneration, promoting cell migration, and enhancing collagen deposition.
2/ Design a fibrin biomaterial that rapidly forms a stable blood clot, reducing bleeding and promoting hemostasis in surgical or traumatic injuries.
3. Ensure the fibrin biomaterial is non-toxic, non-immunogenic, and biodegradable, minimizing adverse reactions and promoting tissue integration.
4. Develop a fibrin biomaterial with adjustable degradation rates to match various wound healing processes and tissue regeneration requirements.

SUMMARY

1. Wound healing is a complex process that involves the body's natural to tissue injury and its refers to a living organisms replacement of destroyed or damaged tissue by newly produced tissue. In undamaged skin, the epidermis and dermis form a protective barrier against the external environment and also blood clot is
a vital process that prevents excessive bleeding when a blood vessel is injured. It occurs when platelets and proteins in the blood work together to form a clot over the damaged area. The body usually dissolves the clot once the injury has
healed.

2. Fibrinogen and thrombin are proteins that play important roles in blood clotting and hemostatic a proteins produced in the liver that circulates in the blood plasma. Fibrinogen is a risk factor for thrombosis and is involved in a complex interplay with thrombin and fibrin of protein produced during coagulation that converts fibrinogen into fibrin which forms blood clot to stop bleeding.

3. The development of biomaterial fibrin, a natural biopolymer, plays a crucial role in wound healing and in addition to its important role in hemostasis due to their ability to mimic the natural coagulation process and promote tissue
regeneration.

4. Fibrin, a protein formed during the blood clotting cascade serves as a scaffold that facilitates the repair of damaged tissue. Fibrin is formed from fibrinogen when the enzyme thrombin is activated. Fibrin fibers from a mesh like structure that traps red blood cells and platelets stabilizing the blood clot. Fibrin forms the temporary matrix for cell attachment, migration and tissue repair, it supports angiogenesis (formation of New blood vessels) and the migration of fibroblasts and keratinocytes, key cells in tissues repair

CONCLUSION
The field of fibrin biomaterials for wound healing and blood clotting holds transformative potential, bridging natural healing mechanisms with innovative biomaterial design. By enhancing the regenerative and hemostatic capabilities of
fibrin, researchers are working towards advanced solutions for complex wounds, surgical repair, and trauma care. This exploration not only seeks to improve current treatment outcomes but also to pave the way for personalized and more effective regenerative therapies in medicine.

Advantages of creating this are as follows:
These advantages aim to enhance the quality of life for patients by providing greater mobility and independence while also easing the workload for caregivers.
1. Films are flexible and retain moisture. They provide the ability to monitor wounds visually. Films are semi-permeable, thus allowing for gas exchange while not allowing for external bacteria to enter the wound. These are selfadhesive.
2. Faster healing: Keeping a wound moist can speed up healing by three to five times
3. Lower risk of infection: A moist wound environment creates a barrier that can help reduce the risk of infection.
4. Reduced pain: A moist environment can help prevent the wound from drying out, which can minimize discomfort.
5. Painless plaster removal: A moist wound environment can make it easier to remove or change a plaster without pain.

CLAIMS
WeClaim,
1. Rapid Bleeding Control: This fibrin biomaterial accelerates clot formation to manage bleeding
quickly, ensuring stable hemostasis during both surgeries and emergencies.

2. Enhanced Wound Healing: With a scaffold that encourages cell attachment and growth, it boosts
collagen production and speeds up wound closure for effective recovery.

3. Body-Friendly and Naturally Degradable: Made from natural fibrinogen and thrombin, the
biomaterial is both biocompatible and biodegradable, promoting safe integration with minimal risk of adverse reactions.

4. Versatile and Customizable: Tailored for flexibility, the fibrin biomaterial's strength, elasticity, and degradation rate can be adjusted to meet the needs of various medical applications-from cardiovascular and orthopedic to dermatological treatment

Documents