CLOEME VISCOSA ENRICHED (CVE) MEDIUM FOR TRICHODERMA VIRIDE

Abstract

This invention presents a Cloeme viscosa enriched medium tailored for Trichoderma viride, enhancing its growth, sporulation, and biocontrol efficacy. The CVE medium combines specific nitrogen, carbon sources, and trace minerals to support the metabolic needs of T. viride. By promoting sustained growth and metabolite production, this medium offers an improved solution for agricultural and environmental applications, supporting sustainable biocontrol practices.

Specification

Description:FIELD OF THE INVENTION
This invention relates to agricultural and biotechnological applications, specifically to a nutrient-enriched medium using Cloeme viscosa (CVE) designed to optimize the growth, sporulation, and metabolite production of the biocontrol agent Trichoderma viride. The CVE medium enhances the efficacy of T. viride, supporting its application in biocontrol, agriculture, and environmental biotechnology.
BACKGROUND OF THE INVENTION
Trichoderma viride is a fungal species widely used as a biological control agent in agriculture and environmental biotechnology due to its ability to suppress plant pathogens and promote plant growth. However, conventional media for T. viride cultivation, such as Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA), are not specifically tailored to maximize the growth and biocontrol efficacy of T. viride. These standard media lack essential nutrients and conditions that support optimal growth, sporulation, and production of secondary metabolites, which are critical for its effectiveness in biocontrol applications. Consequently, the limited growth and activity of T. viride on these media restrict its potential impact and efficiency in field applications.
Current research highlights the importance of tailored nutrient profiles that cater to the unique metabolic needs of T. viride. Studies show that specific nitrogen and carbon sources, as well as trace minerals, significantly influence the growth and efficacy of T. viride as a biocontrol agent. However, existing formulations lack the specific nutrient enrichment necessary to maximize these attributes. This invention addresses this gap by introducing an enriched medium formulated with Cloeme viscosa extracts, which provide essential nutrients and growth factors, leading to enhanced biomass production, sporulation, and metabolite synthesis in T. viride. The CVE medium thus represents a novel approach to improving the efficacy of T. viride in various agricultural and environmental applications.
SUMMARY OF THE INVENTION
This summary is provided to introduce a selection of concepts, in a simplified format, that are further described in the detailed description of the invention.
This summary is neither intended to identify key or essential inventive concepts of the invention and nor is it intended for determining the scope of the invention.
To further clarify advantages and features of the present invention, a more particular description of the invention will be rendered by reference to specific embodiments thereof, which is illustrated in the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail with the accompanying drawings.
The invention provides an enriched medium for Trichoderma viride, formulated with Cloeme viscosa extracts and tailored nutrient sources. The CVE medium promotes enhanced growth, sporulation, and metabolite production in T. viride, supporting its efficacy as a biocontrol agent. By optimizing the nutrient profile with nitrogen sources, carbon sources, trace minerals, and bioactive compounds, this medium enhances the commercial and agricultural applications of T. viride, offering a sustainable solution for biocontrol.
DETAILED DESCRIPTION OF THE INVENTION
The detailed description of various exemplary embodiments of the disclosure is described herein with reference to the accompanying drawings. It should be noted that the embodiments are described herein in such details as to clearly communicate the disclosure. However, the amount of details provided herein is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the present disclosure as defined by the appended claims.
It is also to be understood that various arrangements may be devised that, although not explicitly described or shown herein, embody the principles of the present disclosure. Moreover, all statements herein reciting principles, aspects, and embodiments of the present disclosure, as well as specific examples, are intended to encompass equivalents thereof.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms "a"," "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises," "comprising," "includes" and/or "including," when used herein, specify the presence of stated features, integers, steps, operations, elements and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and/or groups thereof.
It should also be noted that in some alternative implementations, the functions/acts noted may occur out of the order noted in the figures. For example, two figures shown in succession may, in fact, be executed concurrently or may sometimes be executed in the reverse order, depending upon the functionality/acts involved.
In addition, the descriptions of "first", "second", "third", and the like in the present invention are used for the purpose of description only, and are not to be construed as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may include at least one of the features, either explicitly or implicitly.
Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, e.g., those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The Cloeme Viscosa Enriched (CVE) Medium for Trichoderma viride is an innovative nutrient formulation that enhances the growth, sporulation, and metabolite production of T. viride. The medium preparation begins with the extraction of bioactive compounds from fresh or dried Cloeme viscosa leaves, which are known for their nutrient richness, including proteins, carbohydrates, and phytohormones that promote fungal growth. The extraction process involves washing the plant material, followed by boiling or soaking in distilled water to release the bioactive components. The resulting extract is filtered to remove solid residues, retaining a nutrient-rich liquid phase used in the CVE medium.
The enriched medium combines Cloeme viscosa extract with nitrogen sources like ammonium sulfate or urea, which are critical for protein synthesis and robust fungal growth. Yeast extract is added to supply B-vitamins and amino acids, supporting metabolic activity and enhancing sporulation. Carbon sources such as glucose and starch provide both immediate and sustained energy for T. viride, facilitating balanced growth and activity. Mineral supplements, including calcium carbonate and magnesium sulfate, are incorporated to maintain pH stability and provide essential minerals. Additionally, trace elements like iron, zinc, and manganese are added in chelated forms, supporting enzymatic functions crucial for fungal metabolism.
To further optimize growth, vitamins and growth factors, including thiamine and biotin, are included in the medium to enhance metabolic pathways. The incorporation of hormones, such as auxins and gibberellins derived from Cloeme viscosa, promotes hyphal extension and sporulation, significantly improving the overall biomass yield. Agar-agar may be added as a gelling agent for solid media formulations, enabling plate assays and other laboratory applications. The prepared CVE medium is sterilized through autoclaving and adjusted to a pH of 6.0 to 6.5 for optimal T. viride growth conditions. After cooling, the medium is inoculated with T. viride under sterile conditions, followed by incubation at controlled temperatures to assess mycelial density, sporulation, and secondary metabolite production.
, Claims:1. A nutrient-enriched medium for promoting the growth, sporulation, and metabolite production of Trichoderma viride, comprising Cloeme viscosa extract, nitrogen sources, carbon sources, and trace minerals.
2. The medium as claimed in Claim 1, wherein the Cloeme viscosa extract provides bioactive compounds that support fungal growth and biocontrol efficacy.
3. The medium as claimed in Claim 1, wherein nitrogen sources include ammonium sulfate or urea to promote protein synthesis and fungal biomass.
4. The medium as claimed in Claim 1, wherein yeast extract supplies B-vitamins and amino acids essential for T. viride growth and sporulation.
5. The medium as claimed in Claim 1, wherein carbon sources include glucose for immediate energy and starch for sustained release, supporting balanced fungal growth.
6. The medium as claimed in Claim 1, wherein mineral supplements, including calcium carbonate and magnesium sulfate, provide essential elements for metabolic processes.
7. A method for preparing the enriched medium as claimed in Claim 1, involving Cloeme viscosa extraction, mixing with nutrient components, and pH adjustment.
8. The medium as claimed in Claim 1, wherein it includes trace elements like iron, zinc, and manganese, enhancing enzymatic activity and fungal metabolism.
9. The medium as claimed in Claim 1, wherein it incorporates thiamine, biotin, auxins, and gibberellins to stimulate growth, hyphal extension, and sporulation.

10. The medium as claimed in Claim 1, wherein it supports the commercial application of Trichoderma viride as a sustainable biocontrol agent in agriculture.

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