BIOACTIVE HERBAL CREAM WITH NANOPARTICLES: ASSESSING ITS POTENTIAL FOR SKIN HEALTH AND INFLAMMATION MANAGEMENT

Abstract

This study explores the formulation and potential benefits of a bioactive herbal cream incorporating nanoparticles for skin health and inflammation management. With increasing interest in natural products and nanotechnology in dermatological applications, this research focuses on harnessing the therapeutic properties of herbal ingredients combined with the enhanced delivery capabilities of nanoparticles. The cream contains a blend of plant-based bioactive compounds known for their anti-inflammatory, antioxidant, and antimicrobial properties. By incorporating nanoparticles, such as liposomes or solid lipid nanoparticles, the bioactive compounds are expected to achieve better skin penetration, sustained release, and higher bioavailability. The research assesses the cream's efficacy in managing common skin conditions such as acne, eczema, and psoriasis, as well as its potential to reduce inflammation associated with various skin irritations. In vitro and in vivo studies are conducted to evaluate the cream’s anti-inflammatory effects, skin healing properties, and its ability to improve skin texture and appearance. The study also compares the nanoparticle-based formulation to conventional herbal creams, focusing on differences in skin absorption, effectiveness, and safety profiles. This innovative approach could provide a more effective, targeted solution for skin care, offering an alternative to conventional pharmaceutical treatments. The findings of this study contribute to the growing body of knowledge on the intersection of herbal medicine and nanotechnology, emphasizing the potential for improved skin health management and inflammation control in dermatological care.

Specification

Description:TECHNICAL FIELD
[001]. The development of bioactive herbal creams with nanoparticles represents a promising innovation in skin health and inflammation management. This technical field integrates traditional herbal medicine with advanced nanotechnology to enhance therapeutic efficacy and bioavailability. Nanoparticles serve as efficient carriers, facilitating targeted delivery of bioactive compounds to deeper skin layers, improving absorption, and reducing side effects.
Herbal ingredients, rich in antioxidants and anti-inflammatory agents, synergize with nanotechnology to address skin disorders such as eczema, acne, and psoriasis. Advanced formulations ensure stability, prolonged release, and enhanced penetration of active compounds like flavonoids, terpenoids, and polyphenols. Research focuses on optimizing nanoparticle size, material composition, and encapsulation techniques to improve functionality. Analytical methods like spectrometry, electron microscopy, and in vitro models evaluate cream efficacy. This interdisciplinary approach highlights the potential for developing sustainable, natural remedies for chronic inflammation and skin regeneration, blending science with holistic health principles.
BACKGROUND OF THE INVENTION

[002] Skin disorders and chronic inflammation represent a growing global health concern, driven by environmental factors, lifestyle changes, and an increase in autoimmune conditions. Conventional treatments often rely on synthetic chemicals or corticosteroids, which can have side effects and limited long-term effectiveness. This has spurred interest in alternative therapies that are safe, natural, and effective.
[003] Herbal medicine, with its rich history of treating skin ailments, offers a treasure trove of bioactive compounds known for their antioxidant, antimicrobial, and anti-inflammatory properties. However, challenges such as poor bioavailability, limited skin penetration, and degradation of active compounds hinder their clinical application.
[004] Nanotechnology provides a transformative solution by encapsulating these bioactives into nanoparticles, enabling enhanced stability, targeted delivery, and improved absorption through the skin. This invention integrates herbal bioactives with nanoparticle-based formulations to create an innovative cream that optimizes skin health while minimizing side effects, offering a novel therapeutic approach for inflammation management.
However, the invention has the potential to leverage the power of natural ingredients from herbal to create a novel and effective herbal cream for promoting healthy skin.
SUMMARY OF THE INVENTION
[005] The polyherbal extract of Lagerstroemia speciosa (Banaba), Moringa oleifera seeds, and Mussaenda has garnered attention due to its potential antioxidant and anti-inflammatory properties. Lagerstroemia speciosa, commonly known as Banaba, contains corosolic acid, which has been shown to exhibit glucose-lowering effects, making it beneficial for managing diabetes. Additionally, it possesses strong antioxidant properties that can help neutralize free radicals in the body, thereby reducing oxidative stress. Moringa oleifera seeds are rich in vitamins, minerals, and bioactive compounds, including flavonoids and phenolic acids, which contribute to its potent antioxidant and anti-inflammatory effects. Mussaenda, a lesser-known medicinal plant, is also reported to have bioactive compounds that contribute to its medicinal properties. Combining these three plant extracts into a polyherbal formulation aims to harness their synergistic effects, enhancing the overall therapeutic potential.
[006] To test the efficacy of this polyherbal formulation, several in vitro and in vivo studies have been conducted. In vitro antioxidant assays, such as DPPH, ABTS, and FRAP, have demonstrated that the combined extracts exhibit significant free radical scavenging activity. This indicates a strong potential for reducing oxidative stress when applied topically. In vivo studies using animal models have further validated these findings. For instance, studies on induced inflammation in rats showed a notable reduction in inflammatory markers when treated with the polyherbal formulation. Histopathological examinations of tissue samples from these studies revealed decreased cellular infiltration and edema, corroborating the anti-inflammatory claims. These preclinical findings provide a strong foundation for considering the polyherbal extract as a viable candidate for topical formulations aimed at managing skin conditions characterized by inflammation and oxidative stress.
[007] The formulation of a polyherbal cream incorporating these extracts involves meticulous processes to ensure stability and efficacy. The extract blend is combined with a cream base, typically consisting of emulsifying agents, humectants, and emollients, to create a stable emulsion. The addition of Titanium Dioxide Nanoparticles (TiO2 NPs) further enhances the formulation. TiO2 NPs are known for their UV-blocking properties, which provide an added benefit of sun protection, making the cream multifunctional. The nanoparticles also contribute to the aesthetic appeal of the cream, improving its texture and consistency. Ensuring that the nanoparticles are uniformly distributed within the cream base is crucial to maintain consistency and efficacy. Advanced formulation techniques, such as high-shear homogenization and ultrasonication, are employed to achieve a homogenous mixture.
The formulated cream undergoes rigorous testing to evaluate its safety, stability, and efficacy. Stability tests include assessing the physical and chemical properties of the cream under various conditions, such as different temperatures and humidity levels, to ensure it remains effective over its shelf life. Safety assessments involve testing for skin irritation and sensitization in both in vitro models and human volunteers. Clinical trials are essential to establish the efficacy of the cream in humans. In these trials, participants with skin conditions characterized by oxidative stress and inflammation, such as eczema or psoriasis, apply the cream over a specified period. Clinical outcomes are measured using standardized dermatological assessment tools, and results consistently show improvements in skin condition, reduced inflammation, and enhanced skin barrier function. These clinical studies are crucial for validating the preclinical findings and confirming the therapeutic potential of the polyherbal cream.
In conclusion, the integration of Lagerstroemia speciosa, Moringa oleifera seeds, and Mussaenda into a polyherbal cream formulation, augmented with Titanium Dioxide Nanoparticles, presents a promising approach for managing skin conditions characterized by inflammation and oxidative stress. The rigorous testing of the formulation, including in vitro antioxidant assays, in vivo anti-inflammatory studies, and comprehensive clinical trials, supports its safety and efficacy. The multifunctional benefits of the cream, including antioxidant, anti-inflammatory, and UV-blocking properties, make it a valuable addition to dermatological therapeutics. Continued research and development in this area could further enhance the formulation, optimizing its therapeutic potential and broadening its application scope in skincare and medical dermatology.
BRIEF DESCRIPTION OF THE DRAWINGS
[008] The embodiments of the invention will be better understood with the detailed description, by way of non-limiting examples and accompanying drawings in which:
[009] FIG. 1 Antimicrobial Study, Zone of Inhibition for E. Coli, Streptococcus Species for LMM Sample and Ampicillin
[010] FIG. 2 LMM - protein denaturation method
[011] FIG. 3 HRBC Membrane Stabilization Method
[012] FIG. 4 Evaluation of Polyherbal Cream
[TAILED DESCRIPTION
[013] The following is a detailed description of embodiments of the disclosure depicted in the accompanying drawings.
[014] FIG.1 Antimicrobial Study, Zone of Inhibition for E. Coli, Streptococcus Species for LMM Sample and Ampicillin
[015] FIG. 2 Anti Inflammatory activity by LMM - protein denaturation method
[016] FIG. 3-4. Anti-Inflammatory activity by HRBC Membrane Stabilization Method and Evaluation of Polyherbal Cream.
Colour: The cream exhibited a light greenish hue, consistent with the presence of herbal extracts. The colour remained stable over time, indicating good formulation stability. Odour: The odour was pleasant and herbal, with no detectable off-smell. This suggests that the extracts were well-incorporated and no degradation of components occurred.Consistency: The cream had a smooth and uniform consistency, indicating proper formulation techniques. It was neither too thick nor too runny, which is ideal for topical applications. State: The cream remained homogeneous, with no phase separation observed over the evaluation period. This indicates good emulsification and stability of the cream. pH: The pH of the cream was found to be 6.5, which is within the acceptable range for topical applications, ensuring skin compatibility. A neutral to slightly acidic pH is preferred to match the natural pH of the skin. Spreadability: The spreadability was measured to be 7.2 cm, indicating good application properties. The cream spread easily and uniformly, which is important for effective coverage and absorption. Washability: The cream was easily washable with water, suggesting user convenience. Easy removal ensures that the cream does not leave residues or cause discomfort. Non-irritancy test: No signs of irritation or allergic reactions were observed in any of the volunteers, indicating the formulation's safety for topical use. This suggests that the cream is suitable for sensitive skin and regular use The polyherbal cream demonstrated excellent physical properties and user-friendly characteristics. The results indicate that the cream is stable, easy to apply, and safe for use, making it a promising candidate for further pharmacological evaluations and potential therapeutic applications
REFERENCE CITED:
Patent Literature:
1. US20190254987A1: - This patent details methods and compositions for delivering bioactive, nanoencapsulated antioxidants to target tissues
2. ES2345806B1 - This patent describes nanoparticulate systems made from anionic polymers for administering bioactive molecules for cosmetic use.
, Claims:WE CLAIM:
1. In claim 1, Composition Claim: A topical formulation comprising: An effective amount of bioactive herbal extracts selected from [specific herbs known for anti-inflammatory and skin health benefits, e.g., turmeric, neem, aloe vera]; Nanoparticles encapsulating said herbal extracts, wherein the nanoparticles are composed of [specific biocompatible materials, e.g., chitosan, liposomes, or solid lipid nanoparticles]; A pharmaceutically acceptable carrier suitable for topical application.
2. In claim 2, Method of Preparation Claim: A method for preparing the bioactive herbal cream as claimed in claim 1, comprising: Extracting bioactive compounds from [specific herbs] using [specific extraction method, e.g., aqueous or solvent extraction]; Formulating nanoparticles by [specific method, e.g., emulsification, solvent evaporation, or nanoprecipitation] to encapsulate the herbal extracts;
3. In claim 3, Use Claim: Use of the bioactive herbal cream as claimed in claim 1 for: Reducing skin inflammation associated with conditions such as eczema, psoriasis, or acne; Promoting skin regeneration and healing of minor wounds or irritations; Enhancing overall skin health by providing antioxidant and antimicrobial effects.
Dated this: 24th November 2024.