ANTIMICROBIAL UV PROTECTIVE BAMBOO UNION FABRICS AND METHOD OF PREPARATION

Abstract

The present invention relates to the antimicrobial and UV protective bamboo union fabrics with textile finish comprising a textile substrate, anti-microbial plant based metal nanoparticle and binder with antimicrobial activity against E. coli, Bacillus subtilis and Aspergillus niger. The textile finish is incorporated with biosynthesized nanoparticles from extracts of Saraca asoca(Ashoka), Mangifera indica(Mango), Ficus religiosa(Peepal) trees. Further the durability of the fabric of the present invention is assessed.

Specification

Description:FIELD OF INVENTION
The present invention relates to the antimicrobial and UV protective bamboo union fabrics. Further the fabrics are incorporated with plant extracts and biosynthesized nanoparticles that are developed.
BACKGROUND OF THE INVENTION
As there is tremendous development happening in textile technology, the use of textiles is no more limited to protection from the environment, aesthetics, and modesty. Many categories of technical textiles such as, medical textiles, smart textiles, geotextiles, and agri textiles are developed with specific functionality. The textile finish is anything done to fiber, yarn, or fabric before or after fabric construction, to change the fabric's appearance, hand, and performance. Fabrics commercially available in the market are given one or more finishes depending on the end use. Functional finishes are applied to fabrics to improve performance and enhance fabric suitability for a specific purpose. They are also called special purpose finishes. Many functional finishes are topical - they are added to the surface and do not penetrate into the fiber or yarn. Antimicrobial and UV-protective finishes are functional finishes, which have growing demand.
Microbes are omnipresent. They multiply rapidly under suitable conditions such as heat, humidity, and food. The microorganisms could be pathogenic and non-pathogenic. Pathogenic microbes will grow in textiles that come in contact with the human body. They adhere to the fiber, multiply quickly, and finally, damage the fiber. Even if the microbes are non-pathogenic, they can cause undesirable color changes, stains, foul odor, and degeneration of textile fibers. The natural fibers provide favorable conditions for growth of microbes. They consist of starch, protein, and fats or oils, which may be added during the finishing, and provides food for microbes. The synthetic fibers and blends also, when come in contact with human body, immediately absorb perspiration and encourage microbial growth. Textiles are worn next to the skin and act as a barrier between the wearer's body and the external environment. Though they provide protection, they can become a potential breeding ground for microbe growth as well as a medium of transmission. The availability of large surfaces and moisture retaining property of textile materials provide a nourishing environment for microbial growth, causing undesirable effects on the textile as well as the wearer.
The antimicrobial finish for such textiles inhibits the growth of bacteria and prevents decay and damage from perspiration. Textiles are the most suitable materials to impart antimicrobial properties. The antimicrobial finish is used on textiles to control bacteria, fungi, mold, mildew, and algae and the problems of deterioration, staining, odors, and health concerns that they cause. Thus, textile finishes, which impart properties such as anti-fungal, anti-mildew, and anti-bacterial are given to medical textiles, bandages used for wound healing, and protective clothing, creating the need for innovative research in developing antimicrobial textile finishes. A lot of scientific investigations are going on in developing durable antimicrobial agents for a long time. The present invention relates to an antimicrobial and UV protective textile finish on the bamboo union fabrics which makes the textiles antimicrobial in nature.
OBJECTIVE OF THE INVENTION
The objective of the present invention is to prepare antimicrobial and UV protective bamboo union fabrics with textile finish comprising a textile substrate, anti-microbial plant based metal nanoparticles and binder.
An objective of the present invention relates to the union fabrics that is prepared with antimicrobial activity against E. coli, Bacillus subtilis and Aspergillus niger.
Another objective of the present invention relates to the antimicrobial and UV protective textile finish incorporated with biosynthesized nanoparticles.
Yet another objective of the present invention relates to an incorporation of biosynthesized nanoparticles from extracts of Saraca asoca(Ashoka), Mangifera indica(Mango), Ficus religiosa(Peepal) trees.
Another objective of the present invention is to formulate a method to prepare the nanoparticles using metals and tree bark extracts for the antimicrobial textile finish of the union fabrics.
SUMMARY OF THE INVENTION
In an aspect of the present invention relates to the development of Antimicrobial and UV-Protective Bamboo Union Fabrics.
In an aspect of the present invention relates to the antimicrobial and UV protective bamboo union textile finish wherein the textile substrates are three natural fibres, bamboo, cotton and linen woven in to two union fabrics, comprising Bamboo/ Linen union fabric (UF1) with 2/20s count Bamboo yarn in warp and 40 Lea Linen yarn in weft and Bamboo/ Cotton union fabric (UF2) was made with Bamboo 2/20s count warp yarn and 2/20s count Cotton in weft.
In another aspect of the present invention antimicrobial and UV protective textile finish, the plant extracts used for biosynthesis of the nanoparticles are Saraca asoca(Ashoka), Mangifera indica (Mango), Ficus religiosa(Peepal).
Yet another aspect of the present invention relates to the antimicrobial and UV protective textile finish as claimed in claim 1 wherein, antimicrobial metal nanoparticle is synthesized with copper and zinc.
Another aspect of the present invention, antimicrobial and UV protective textile finish as claimed in claim 1 wherein the UPF values of the finished union fabrics are in the range of 32-721.42.
In an aspect the present invention relates to a process to prepare the antimicrobial and UV protective bamboo union fabrics, the steps comprising of:
a. Three natural fibres, bamboo, linen and cotton unbleached 100% pure yarns were procured and two union fabrics UF1 & UF2 were woven and used in the invention;
b. Barks of Ashoka, Mango & Pipal trees are collected, pounded and grinded to a smooth powder. The Ashoka and Mango bark powders were macerated with methanol and acetone respectively, for 24 hrs. For the Pipal bark powder, aqueous extraction was done, yielding three bark extracts;
c. 1.64 gm of Zn (NO3)2 (zinc nitrate) and 0.125 gm of Cu(CH3COO)2 (Cupric Acetate) are added to 100 ml of distilled water and stirred continuously for 15 mins to get Zn/Cu nano solution, to this the bark extract is added in the proportions of 4:1, 1:2, 2:1 & 1:1 and Zn/Cu mixture, pH is adjusted using NaOH solution to yield a nanocomposite solution;
d. The prepared solution is heated in a convection microwave at 15 seconds cycles until all the liquid evaporated, the nano particles thus obtained were washed with water and ethanol and calcinated in a muffle furnace at 500°C for 1 hour and the resulting particles are grinded into a fine powder and stored in air tight bottles for textile application.
e. The nano particles obtained after calcination were dissolved in distilled water to prepare nano particle solution, to which 1% PVA binder solution was added and applied on union fabrics.
DETAILED DESCRIPTION OF THE INVENTION
The following description provides specific details of certain aspects of the disclosure illustrated in the drawings to provide a thorough understanding of those aspects. It should be recognized, however, that the present disclosure can be reflected in additional aspects and the disclosure may be practiced without some of the details in the following description.
The various aspects including the example aspects are now described more fully with reference to the accompanying drawings, in which the various aspects of the disclosure are shown. The disclosure may, however, be embodied in different forms and should not be construed as limited to the aspects set forth herein. Rather, these aspects are provided so that this disclosure is thorough and complete and fully conveys the scope of the disclosure to those skilled in the art. In the drawings, the sizes of components may be exaggerated for clarity.
The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process or method that comprises a list of steps does not include only those steps but may include other steps not expressly listed or inherent to such a process or method. Similarly, one or more components, compounds, and ingredients preceded by "comprises" does not, without more constraints, preclude the existence of other components or compounds or ingredients or additional components. Appearances of the phrase "in an embodiment", "in another embodiment" and similar language throughout this specification may, but not necessarily do, all refer to the same embodiment.
In the following specification and the claims, reference will be made to a number of terms, which shall be defined to have the following meanings. The singular forms "a", "an", and "the" include plural references unless the context clearly dictates otherwise.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this disclosure belongs. The system, methods, and examples provided herein are only illustrative and not intended to be limiting.
In an embodiment the present invention relates to the antimicrobial and UV protective bamboo union fabrics with textile finish comprising a textile substrate, plant based metal nanoparticle and binder to chemically bind to textile substrate wherein the textile article exhibits antimicrobial properties after 15 washes in accordance with AATCC Test Method including the exhibition of zone of inhibition ranging between 0.1-6.26 mm against E. coli, Bacillus subtilis and Aspergillus niger.
In another embodiment the present invention relates to usage of natural substratesare three natural fibres bamboo, cotton and linen woven in two union fabrics comprising Bamboo/ Linen union fabric (UF1) with 2/20s count Bamboo yarn in warp and 40 Lea Linen yarn in weft and bamboo/ Cotton union fabric (UF2) was made with bamboo 2/20s count warp yarn and 2/20s count cotton in weft.
In yet another embodiment of the present invention relates to an antimicrobial and UV protective bamboo union textile finish wherein the plant extracts of the nanoparticles used are Saraca asoca(Ashoka), Mangifera indica (Mango), Ficus religiosa (Peepal).
In an embodimment the present invention relates to a process to prepare an antimicrobial and UV Protective bamboo union fabric with textile finish, the steps comprising of:
a. Three natural fibres, bamboo, linen and cotton unbleached 100% pure yarns were procured and two union fabrics UF1 & UF2 were woven and used in the invention;
b. Barks of Ashoka, Mango & Pipal trees are collected, pounded and grinded to a smooth powder. The Ashoka and Mango bark powders were macerated with methanol and acetone respectively, for 24 hrs. For the Pipal bark powder, aqueous extraction was done, yielding three bark extracts;
c. 1.64 gm of Zn (NO3)2 (zinc nitrate) and 0.125 gm of Cu(CH3COO)2 (Cupric Acetate) are added to 100 ml of distilled water and stirred continuously for 15 mins to get Zn/Cu nanoparticles, to this the bark extract is added in the proportions of 4:1, 1:2, 2:1 & 1:1 of tree bark extracts and Zn/Cu mixture, pH is adjusted using NaOH solution to yield a nanocomposite solution.
d. The prepared solution is heated in a convection microwave at 15 seconds cycles until all the liquid evaporated, washed with water and ethanol and calcinated in a muffle furnace at 500°C for 1 hour and the resulting particles are grinded into a fine powder and stored in air tight bottles for textile application.
e. The nano particles obtained after calcination were dissolved in distilled water to prepare nano particle solution, to which 1% PVA binder solution was added and applied on union fabrics.
While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.





EXAMPLES
The present invention is further explained in the form of the following examples. However, it is to be understood that the following examples are merely illustrative and are not to be taken as limitations upon the scope of the invention.
Example 1: Materials
Three natural fibres, bamboo, linen and cotton unbleached 100% pure yarns were procured and two union fabrics were woven on a frame loom at master weaver's unit, Sathenapally, Andhra Pradesh, India. The Bamboo/ Linen union fabric (UF1) was woven with 2/20s count Bamboo yarn in warp and 40 Lea Linen yarn in weft. The bamboo/ Cotton union fabric (UF2) was made with bamboo 2/20s count warp yarn and 2/20s count cotton in weft.
The Mango, Ashoka and Pipal barks were procured from local market. The barks were pounded in to small pieces in a stone grinder and made in to a smooth powder, using a mixer grinder, the obtained powders were sieved several times to remove big particles, until smooth, fine powders were filtered.
Example 2: Extraction of Antimicrobial agents from Barks
Three methods of extraction, viz., Soxhlet extraction, aqueous extraction and maceration were tested. Maceration was selected over Soxhlet extraction, due to high yield, low cost, ease of extraction. Maceration was carried out with two solvents, methanol and acetone in three different concentrations, viz, 5%, 10% and 20%, using a magnetic stirrer at 550RPM for 24 hours and the solution was kept aside for 8 hours. The supernatant liquid was filtered with Whatman filter paper and stored in a refrigerator for further use. The aqueous extraction was conducted by taking distilled water as solvent along with bark powder, on a hot plate heated to 99°C, heated for 30 minutes. The solution was cooled down to room temperature, filtered and refrigerated.
Example 3: Synthesis and analysis of nanocomposite materials
Biosynthesis of nanocomposites
Nanoparticles of two metals i.e. Copper & Zinc, which have very good antimicrobial properties were selected. 1.64 gm of Zn (NO3)2 (zinc nitrate) and 0.125 gm of Cu(CH3COO)2 (Cupric Acetate) were added to 100 ml of distilled water and stirred continuously for 15 mins. Using a magnetic stirrer at 550 RPH. Then measured quantity of bark extract was added to the premeasured nano solution of Zn and Cu. Then the pH of the solution was adjusted to above 7 PH (alkaline) by adding required quantity of NaOH(Sodium Hydroxide) solution (1.995%).
The nano particles were synthesized using different proportions of optimized concentrations of tree bark extracts and the chemicals. The proportions and sample names are furnished in Table 1.
Name of the sample nanoparticle Ratios Proportions
A1 4:1 80/20
A2 1:2 33/66
A3 1:1 50/50
A4 2:1 66/33
P1 4:1 80/20
P2 1:2 33/66
P3 1:1 50/50
P4 2:1 66/33
M1 4:1 80/20
M2 1:2 33/66
M3 1:1 50/50
M4 2:1 66/33
Table 1: Different proportions of optimized concentrations of tree bark extracts and the chemicals.
Microwave assisted synthesis
The prepared solution was heated in a convection microwave at 15 seconds cycles until all the liquid evaporated and dried solid particles remained at the bottom of the beaker. The solid particles obtained were cooled, collected and washed several times with distilled water, followed by washing three times with ethanol. The washing process was done by placing the nano particles on a filter paper over a funnel. The filter paper containing the nano particles was kept in a hot air oven at 90°C for 24 hours.
Calcination: The dried nano particles were collected in a crucible, covered with aluminum foil and kept at 500°C for one hour using a muffle furnace. The furnace was allowed to cool and the nano particles were hand grinded in to fine powder in lab mortar and pestle.


XRD Tests to study Nanoparticle structure and size:
XRD Analysis (Table 2, 3 &4) was conducted to find out the nanoparticle size and lattice strain values. Smaller the nanoparticle, higher the antimicrobial efficacy.

Name of the NP Proportions of bimetallic Ashoka Extract NPs CRYSTALLITE SIZE (NM) Lattice strain (%) Structure of NP
A1 80/20 24.83 0.576 Cubic structure
A2 33/66 27.2 0.731 Cubic structure
A3 50/50 21.01 0.462 Cubic structure
A4 66/33 22.1 0.401 Cubic structure
Table.2: XRD analysis of Ashoka Extract biosynthesized Nano particles

Name of the NP Proportions of bimetallic Mango Extract NPs Crystallite size (NM) Lattice strain (%) Structure of NP
M1 80/20 16.3 0.505 Cubic structure
M2 33/66 15.5 0.349 Cubic structure
M3 50/50 14.1 0.377 Cubic structure
M4 66/33 19.68 0.064 Cubic structure
Table.3:XRD analysis of Mango Extract biosynthesized Nano particles
Name of the NP Proportions of bimetallic Pipal Extract NPs Crystallite size (NM) Lattice strain (%) Structure of NP
P1 80/20 17.55 0.493 Cubic structure
P2 33/66 17.45 0.482 Cubic structure
P3 50/50 17.02 0.621 Cubic structure
P4 66/33 20.87 0.421 Cubic structure

Table.4: XRD analysis of Pipal Extract biosynthesized Nano particles

Example 4: Antimicrobial Tests
The treated samples were tested for antibacterial activity against E. coli and Bacillus subtilis, using the test method AATCC 147-2004 parallel streak method and the antifungal efficacy was tested against Aspergillus niger with the test method AATCC 30-2004-disc diffusion method. According to AATCC test method 147-2004, the samples were cut to a size of 25mm X 50mm rectangle pieces. The nutrient agar was prepared and poured into sterile petri plates and allowed to cool. The inoculum of E. coli streaked in five parallel lines on the petri plates. The fabric sample was gently placed on these parallel streaks and incubated at 37 ± 2°C (99 ± 4°F) for 18-24 hours. The petri plates were evaluated for interrupted growth and clear Zone of Inhibition (ZOI in mm) along the streak. The average ZOI was calculated by the equation
W = (T - D)/2
W= Width of the clear zone of inhibition (mm)
D= Diameter of the specimen (mm)
T= total diameter of the clear zone including test specimen diameter(mm)
The ZOI of each streak was assessed and an average value was reported. The same method was followed for testing antibacterial activity against Bacillus subtilis also.
The antifungal activity against Aspergillus niger was tested using AATCC 30 test method, disc diffusion. The control and treatment samples of 10 mm diameter circles were cut. The petri plates were poured with nutrient agar. The inoculum of Aspergillus niger was streaked on the petri plates. The fabric discs were gently placed on the petri plates. The petri plates were kept aside at room temperature for 7 days and the area of discs covered with Aspergillus niger growth were observed and reported in percentage. Four standards were assigned based on the percentage of fungus growth. They are given below.

% of growth of Aspergillus niger Standard rating
0% / no growth 4
Less than 10% growth 3
10% - 30% growth 2
60% - 90% growth 1
90%-100% Growth 0
Table 5: Antifungal Tests Against Aspergillus Niger - Ratings for Assessment of Results


NP E. coli (ZOI in mm) Bacillus subtilis (ZOI in mm)
CUF1 TUF1 CUF2 TUF2 CUF1 TUF1 CUF2 TUF2
A1 0.00 4.20 0.00 2.00 0.00 1.40 0.00 2.40
A2 0.00 3.00 0.00 1.60 0.00 2.00 0.00 3.40
A3 0.00 4.40 0.00 2.60 0.00 4.00 0.00 4.20
A4 0.00 4.20 0.00 2.20 0.00 1.20 0.00 1.80
M1 0.00 2.00 0.00 5.40 0.00 1.60 0.00 2.80
M2 0.00 2.20 0.00 5.20 0.00 5.20 0.00 3.60
M3 0.00 3.80 0.00 6.40 0.00 5.00 0.00 4.80
M4 0.00 2.40 0.00 2.80 0.00 3.00 0.00 3.00
P1 0.00 2.60 0.00 2.80 0.00 3.20 0.00 3.40
P2 0.00 3.20 0.00 3.80 0.00 3.00 0.00 4.00
P3 0.00 3.80 0.00 3.80 0.00 4.80 0.00 4.00
P4 0.00 2.80 0.00 4.40 0.00 1.60 0.00 6.40

Table 6: Antibacterial Tests Results

Based on the above antibacterial test results, A3, M3 and P3nano particles were selected for textile finishing, to conduct UV resistance tests, and antifungal tests. The antifungal test results are given below.
NP A. niger (Rating)
CUF1 TUF1 CUF2 TUF2
A3 0 3 0 3
M3 0 4 0 2
P3 0 4 0 4

Table 7: Antifungal Tests Results

Example 5: Washing Tests of Treated Samples - Test for Durability
Wash fatness tests were conducted with a Launder-O-meter to test the durability of the antimicrobial finish after 1 wash, 5 washes, 10 washes and 15 washes, followed by antibacterial tests against E. coli and Bacillus subtilis and antifungal tests against Aspergillus niger. The antimicrobial efficacy of the samples was tested after each set of washes.

NP No. of washes E. coli (ZOI in mm) Bacillus subtilis (ZOI in mm) A. niger (Rating)
CUF1 TUF1 CUF2 TUF2 CUF1 TUF1 CUF2 TUF2 CUF1 TUF1 CUF2 TUF2
A3 1 0.00 4.00 0.00 2.20 0.00 3.70 0.00 4.00 0 1 0 1
5 0.00 3.60 0.00 1.90 0.00 2.90 0.00 3.40 0 1 0 1
10 0.00 2.50 0.00 0.80 0.00 1.70 0.00 2.80 0 1 0 1
15 0.00 1.50 0.00 0.30 0.00 0.90 0.00 1.65 0 0 0 1
M3 1 0.00 3.20 0.00 6.00 0.00 4.30 0.00 4.00 0 4 0 4
5 0.00 2.80 0.00 5.00 0.00 3.60 0.00 3.10 0 4 0 2
10 0.00 2.00 0.00 4.10 0.00 2.40 0.00 2.20 0 4 0 2
15 0.00 1.30 0.00 3.40 0.00 1.80 0.00 1.30 0 2 0 2
P3 1 0.00 3.20 0.00 3.30 0.00 4.00 0.00 3.50 0 4 0 3
5 0.00 2.70 0.00 2.50 0.00 3.20 0.00 2.90 0 1 0 2
10 0.00 1.90 0.00 1.60 0.00 2.70 0.00 2.00 0 1 0 2
15 0.00 0.70 0.00 0.50 0.00 1.90 0.00 1.20 0 1 0 2

Table:8 Antimicrobial property durability to washing
Example 6: Ultra Violet protection Factor
The ratio of the average effective UVR irradiance calculated for unprotected skin to the average effective UVR irradiance calculated for skin protected by the test fabric.
Ultra Violet resistance determines the extent of blocking or transmission of ultraviolet radiation through textile fabrics intended to be used for UV protection. The ability of a fabric to block UV light is given by the ultraviolet protection factor (UPF). The UV-A, UV-B and UPF values of all the treated and untreated samples were analyzed as per Australia - New Zealand test method AS/NZS 4399:1996 using a Shimadzu UV3101 PC (UV-VIS-NIR scanning spectrophotometer) at Textile Laboratory and Research Centre, Textile Committee, Mumbai. The UV rays passed through the fabric and collected by detector were measured. The UPF value of the fabric was determined from the total spectral transmittance using the following equation:

400
∑ EλSλΔλ
290
UPF= --------------------------------
400
∑ EλSλTλΔλ
290

Where, Eλ is the relative erythemal spectral effectiveness (unitless),
Sλ is the solar UVR spectral irradiance in W.m-2.nm-1,
Tλ is the measured spectral transmission of the fabric,
Δλ is the bandwidth in millimeter
λ is the wavelength in is the wavelength in nanometer
The analyzed samples were rated following the standard category for UPF ratings and protection as per the test method. The details of the ratings are given below.

UPF rating Protection category % UV radiation transmitted
15-24 Good 6.7-4.2
25-39 Very good 4.1-2.6
40-50+ Excellent ≤ 2.5
Table 9: Standard category for UPF ratings
The UV protection functionality of the biosynthesized nano particles, A3, M3, and P3 were tested and rated based on the calculated UPF values. The results are furnished below.

NP Type of sample % transmittance (UFA) % Transmittance (UFB) UPF Value UPF Range UPF Rating
CUF1 25.20 24.60 23 Below 25 good
CUF2 25.60 27.71 25 Below 25 good
A3 A3UF1 26.70 3.77 57.96 50+ Excellent
A3UF2 32.09 31.33 47.60 40-50+ Excellent
M3 M3UF1 46.98 23.65 51.17 50+ Excellent
M3UF2 52.41 32.76 32.83 25-39 Very Good
P3 P3UF1 58.06 35.98 45.34 40-50+ Excellent
P3UF2 51.21 39.12 70.29 50+ Excellent
Table. 10. Assessment of UV protective property of Nano finishes
NP Name of the sample Number of washes % transmittance (UFA) % transmittance (UFB) UPF Value UPF Range UPF Rating
A3 A3UF1 1W 64.05 54.87 115.92 50+ Excellent
A3UF2 21.46 15.25 32.48 25-39 Very Good
A3UF1 5W 67.14 54.60 71.75 50+ Excellent
A3UF2 38.60 23.77 63.46 50+ Excellent
A3UF1 10W 52.53 27.05 63.46 50+ Excellent
A3UF2 52.53 27.05 77.43 50+ Excellent
A3UF1 15W 18.20 24.26 50.33 50+ Excellent
A3UF2 57.82 33.26 35.51 25-39 Very Good
M3 M3UF1 1W 44.30 48.98 721.42 50+ Excellent
M3UF2 40.12 0.00 360.02 25-39 Very Good
M3UF1 5W 39.05 1.78 496.25 50+ Excellent
M3UF2 32.28 0.04 333.79 50+ Excellent
M3UF1 10W 23.91 2.32 288.85 50+ Excellent
M3UF2 26.57 4.53 186.14 50+ Excellent
M3UF1 15W 23.89 8.23 256.05 50+ Excellent
M3UF2 31.44 4.39 89.29 50+ Excellent
P3 P3UF1 1W 66.77 49.39 47.24 40-50+ Excellent
P3UF2 66.00 45.00 42 50+ Excellent
P3UF1 5W 100.00 100.00 45.46 40-50+ Excellent
P3UF2 65.40 38.54 40.72 40-50+ Very Good
P3UF1 10W 66 49 40 50+ Excellent
P3UF2 100.00 100.00 95.35 50+ Excellent
P3UF1 15W 39.28 175.55 73.37 50+ Excellent
P3UF2 39.90 23.73 38.29 25-39 Very Good

Table. 11. Assessment of durability of UV protective property of Nano finishes to washing

A skilled artisan will appreciate that the quantity and type of each ingredient can be used in different combinations or singly. All such variations and combinations would be falling within the scope of present disclosure.
The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
, Claims:1. The antimicrobial and UV protective bamboo union fabrics with textile finish comprising a textile substrate, antimicrobial plant-based metal nanoparticle and binder to chemically bind to textile substrate wherein the textile article exhibits antimicrobial properties after 15 washes in accordance with AATCC Test Method including the exhibition of zone of inhibition ranging between 0.1-6.26 mm against E. coli, Bacillus subtilis and Aspergillus niger.
2. The antimicrobial and UV protective bamboo union fabrics as claimed in claim 1, wherein the textile substrates are three natural fibres bamboo, cotton and linen woven in two union fabrics comprising bamboo/ linen union fabric (UF1) with 2/20s count bamboo yarn in warp and 40 lea linen yarn in weft and bamboo/ cotton union fabric (UF2) made with bamboo 2/20s count warp yarn and 2/20s count cotton in weft.
3. The antimicrobial and UV protective bamboo union fabrics as claimed in claim 1, wherein the plant-based nanoparticles are prepared from the extracts of Saraca asoca (Ashoka), Mangifera indica (Mango), Ficus religiosa (Pipal).
4. The antimicrobial and UV protective bamboo union fabrics as claimed in claim 1, wherein the metals used in preparing antimicrobial metal nanoparticle are copper and zinc.
5. A process to prepare the antimicrobial and UV protective bamboo union fabrics with textile finish as claimed in claim 1, the steps comprising of:
a. three natural fibres, bamboo, linen and cotton unbleached 100% pure yarns were procured and two union fabrics UF1 & UF2 were woven and used in the invention;
b. barks of Ashoka, Mango & Pipal trees are collected, pounded and grinded to a smooth powder, the Ashoka and Mango bark powders were macerated with methanol and acetone respectively, for 24 hrs and for the Pipal bark powder, aqueous extraction was done, yielding three bark extracts;
c. 1.64 gm of Zn (NO3)2 (zinc nitrate) and 0.125 gm of Cu(CH3COO)2 (Cupric Acetate) are added to 100 ml of distilled water and stirred continuously for 15 mins to get Zn/Cu nanoparticles, to this the bark extract is added in the proportions of 4:1, 1:2 & 1:1 of tree bark extracts and Zn/Cu mixture, pH is adjusted using NaOH solution to yield a nanocomposite solution;
d. the prepared solution is heated in a convection microwave at 15 seconds cycles until all the liquid evaporated, washed with water and ethanol and calcinated in a muffle furnace at 500°C for 1 hour and the resulting particles are grinded into a fine powder;
e. the nano particles obtained after calcination are dissolved in distilled water to prepare nano particle solution, to which 1% PVA binder solution is added and applied on union fabrics.

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