AN INSECTICIDAL COMPOSITION

Abstract

ABSTRACT AN INSECTICIDAL COMPOSITION The present invention relates to an insecticidal composition. In particular, it relates to Compound A: 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl)isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide, Compound B: 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl)thiourea and Compound C: 2-[1-(4-phenoxyphenoxy)propan-2-yloxy]pyridine demonstrating an effective and synergistic broad spectrum control of insects-pests and mites with one shot application by developing delay in resistance of hard to kill and resistant towards insects-pests and mites. Further, the insecticidal composition provides residual control and increases yield of treated plants due to protection against insect-pests and mites. The insecticidal composition of present invention increases overall health and plant vigor and is environmentally safe.

Specification

Description:FORM 2
THE PATENTS ACT 1970
(39 of 1970)
&
The Patents Rules, 2003
COMPLETE SPECIFICATION
(See section 10 and rule 13)
1. TITLE OF THE INVENTION: "AN INSECTICIDAL COMPOSITION"
2. APPLICANT:
(a) Name : BPI CHEMTEX PVT. LTD.
(b) Nationality : INDIAN
(c) Address : 138, G. V. M. M. Industrial Estate, Odhav, Ahmedabad 382 415, Gujarat, INDIA.
PROVISIONAL
The following specification describes the invention.  COMPLETE
The following specification particularly describes the invention and the manner in which it is to be performed.


FIELD OF INVENTION
The present invention relates to an insecticidal composition. More specifically, the present invention relates to an insecticidal composition comprising Compound A: 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl)isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide, Compound B: 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl)thiourea and Compound C: 2-[1-(4-phenoxyphenoxy)propan-2-yloxy]pyridine; and a process of preparing said composition. The present invention further relates to an insecticidal composition that provides an effective and synergistic control to broad spectrum of insect pests and mites with one shot application. Further, the insecticidal composition of present invention increases yield and vigor of the plant by providing residual control and increment in tolerance against insect pests and mites.

BACKGROUND OF THE INVENTION
In recent years, the problem of pest resistance in agricultural production is a global problem and has always been a key topic of concern for agricultural science and technology workers. Further, with the continuation of the use of chemical insecticides to control pests year after year, and the increase in the use of insecticides as well as the unscientific use of insecticides and other factors, the resistance of pests is becoming more and more serious, and the types of resistant pests are increasing.
At the same time, the high-intensity use of insecticides has led to a series of problems such as excessive insecticides residues in agricultural products, pollution of the environment, and increased costs for farmers to use drugs, which is not conducive to the sustainable development of agriculture.
The long-term use of single chemical insecticides will form a directional choice for insects, pests and mites which will increase the resistance of insects, pests and mites. Moreover, due to long-term use of chemical insecticides, insects, pests and mites have become more resistant to insecticides.
Patent number 372736 relates to the pesticidal compositions against sucking pests complex comprising bioactive amounts of diafenthiuron, pyriproxyfen and at least one agrochemically active insecticides selected from thiamethoxam, imidacloprid, thiacloprid, dinotefuran, clothianidin and pymetrozine.
Patent number 372550 relates to the synergistic miticidal composition comprising pyriproxyfen, diafenthiuron, and at least one (C8-C16) alkyl amide as an organic solvent, and wideninig spectrum of action on a plurality of mite's family with an increased safety upon use.
Patent Application number 202411009947 relates to the synergistic insecticidal composition comprising an isoxazoline insecticide and at least two insecticides. In particular, it provides a synergistic insecticidal composition comprising of fluxametamide, pyriproxyfen and flonicamid.
The active ingredients known from the prior arts have certain disadvantages such as insufficient control efficacy, restriction of its use due to the appearance of drug-resistant insects, pests and mites phytotoxicity and contamination to plants, or toxicity to human beings, fishes and the like.
From the above shown prior arts it is also pertinent to note that none of them shows the combination of the compounds and its effective and synergistic approach to control insects, pests and mites as shown by the present invention.
The insecticides currently in use are not that effective; and due to their prolonged indiscriminate and non-judicious use, some pests have developed resistance to such commonly used insecticides. Their use is thereby becoming increasingly difficult.
However, insecticides with different action mechanisms can be mixed reasonably according to a certain proportion, which can improve the efficacy, reduce the dosage, fewer residues, reduce environmental pollution, and effectively reduce the drug resistance of crops against insect pests and mites.
Therefore, there is an urgent need to develop new methods and formulations for controlling these harmful pests. The present inventors have surprisingly developed an effective insecticidal combination which ameliorates the aforesaid shortcomings of the prior art comprising high-efficiency, low-toxicity and environmentally-friendly insecticides which has become a mainstream direction in the field of insecticides research.

OBJECT OF THE INVENTION
The principal object of the present invention is to provide an insecticidal composition.
Another object of the present invention is to provide an insecticidal composition comprising bioactive amounts of Compound A: 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl)isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide, Compound B: 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl)thiourea, Compound C: 2-[1-(4-phenoxyphenoxy)propan-2-yloxy]pyridine and agrochemically acceptable excipients; and a method of preparation thereof.
Further object of the present invention is to provide an insecticidal composition demonstrating effective and synergistic control.
Yet another object of the present invention is to provide an insecticidal composition providing delay in development of resistance and effective control of hard to kill and resistant insect-pests and mites.
Still another object of the present invention is to provide an insecticidal composition which increases yield of treated plants.
Further object of the present invention is to provide an insecticidal composition having residual control by providing longer duration of control with immediate crop protection.
Another object of the present invention is to provide a method of preparing a stable and non-phytotoxic formulation which improves overall health, yield and vigor of the treated plant.
Further object of the present invention is to provide an insecticidal composition which increases plant vigor, is tolerant against insect-pests and mites and is environmentally safe.

SUMMARY OF THE INVENTION
The present invention provides an insecticidal composition for insects, pests and mites. Further, the insecticidal composition comprises Compound A: 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl)isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide; Compound B: 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl)thiourea; Compound C: 2-[1-(4-phenoxyphenoxy)propan-2-yloxy]pyridine; and agrochemically acceptable excipients.

The insecticidal composition of the present invention can be formulated as dry formulation or liquid formulation. Dry formulation includes Dustable powder (DP), Water soluble powder (SP), Wettable powder (WP), Wettable powder packed in water soluble pouch, Water dispersible granules (WDG/WG), Water soluble granule (SG), Emulsifiable granule (EG), Tablets (water soluble/dispersible), Oil dispersible powder (OP). The liquid formulations includes Capsule suspension (CS), Dispersible concentrate (DC), Emulsifiable concentrate (EC), Emulsion water-in-oil (EO), Emulsion oil-in-water (EW), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Suspension concentrate (SC), Suspo-emulsion (SE), Soluble concentrate (SL), Spreading oil (SO), Ultra-low volume (ULV) suspension, Ultra-low volume (ULV) liquid, Zeon Concentrate (ZC)-mixed formulation of CS and SC, Zoldine (ZE)-mixed formulation of CS and SE, ZW-mixed formulation of CS and EW.

The process for preparing the present insecticidal composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However, all such variation and modification is still covered by the scope of present invention.
The present invention is formulated to control broad spectrum control of harmful insect pests (caterpillars, sucking pests) and mites with one shot application. Further, the insecticidal composition provides effective and synergistic control i.e. grams of active ingredients required are less to achieve desired level of control.
Moreover, the insecticidal composition increases delay in development of resistance and effective control of hard to kill and resistant insect-pests and mites. Furthermore it also increases the yield of the treated plants by increasing plant vigor and tolerant against insects, pests and mites.

DETAILED DESCRIPTION OF THE INVENTION
Before explaining the present invention in detail, it is to be understood that the invention is not limited in its application to the details of the parts illustrated. The invention is capable of other embodiments, as described above and of being practiced or carried out in a variety of ways. It is to be understood that the phraseology and terminology employed herein is for the purpose of description and not to limitation. The invention may have various embodiments and they may be performed as described in the following pages of the complete specification.
The terms and words used in the following description are not limited to the bibliographical meanings, but, are merely used by the inventors to enable a clear and consistent understanding of the invention. Accordingly, it should be apparent to those skilled in the art that the following description of exemplary embodiments of the present invention are provided for illustration purpose only and not for the purpose of limiting the scope of the invention.
It is to be understood that the singular forms "a," "an," and "the" include plural reference unless the context clearly dictates otherwise.
Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.
It should be emphasized that the term "comprises/comprising" when used in this specification is taken to specify the presence of stated features, steps or Compounds but does not preclude the presence or addition of one or more other features, steps, compounds or groups thereof.
The term 'plants' as used herein, refers to all physical parts of a plant, including seeds, seedlings, saplings, roots, tubers, stems, stalks, foliage and fruits. The term "plant" is to be understood as including wild type plants and plants, which have been modified by either conventional breeding, or mutagenesis or genetic engineering, or by a combination thereof.
The term "crop" refers to both, growing and harvested crops.
The term "insects" as used herein, includes all organisms in the class "Insecta."
The term "animal pest" includes arthropods, gastropods, and nematodes. Preferred animal pests according to the invention are arthropods, preferably insects and arachnids, in particular insects. Insects, which are of particular relevance for crops, are typically referred to as crop insect pests.
The term "insecticidal" as used herein, refers to the ability of an insecticide to increase mortality or inhibit growth rate of insects.
To "control" or "controlling" pests means to inhibit, through a toxic effect, the ability of pests to survive, grow, feed, and/or reproduce, or to limit pest related damage or loss in crop plants. To "control" pests may or may not mean killing the pests, although it preferably means killing the pests.
The term "health of a plant" or "plant health" is defined as a condition of the plant and/or its products. As a result of the improved health, yield, plant vigor, quality and tolerance to abiotic or biotic stress are increased.
"Yield" is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits in the proper sense, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals).
"Increased yield" of a plant, in particular of an agricultural, silvicultural and/or horticultural plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the composition according to the invention.
The present invention provides an insecticidal composition comprising
1. Compound A: 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl) isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide,
2. Compound B: 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl) thiourea,
3. Compound C: 2-[1-(4-phenoxyphenoxy) propan-2-yloxy] pyridine, with the following mass percentage of the composition:

Sr. No. Ingredient Concentration range (w/w %)
1. Compound A 2 to 10
2. Compound B 15 to 35
3. Compound C 4 to 10

Compound A is Fluxametamide having chemical name as 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl)isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide is a novel wide-spectrum insecticide that was discovered and synthesized by Nissan Chemical Industries, Ltd. It belongs to a class of compounds called isoxazolines, which are potent inhibitors of γ-aminobutyric acid (GABA), glutamate, and glycine-gated chloride channels in insects, and exhibit high insecticidal activity against a variety of insect species, such as lepidoptera, thysanoptera, acarina, and diptera.

Compound A is a wide-spectrum isoxazoline insecticide effective against a broad spectrum of pests. It is mainly used in the control of lepidopteran pests, thrips, whiteflies, leaf miners, beetles and mites on crops such as fruit trees, vegetables, soybeans, cotton and tea trees and other crops.
Compound B is Diafenthiuron having chemical name as 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl)thiourea. It is a chemical compound which belongs to the thiourea group. It is an insecticide and acaricide and contains contact, stomach poison, systemic and fumigation effects.

Compound B is an active agent against a wide range of sucking insects and some lepidopteron pests. Further, it can control aphids and whiteflies in cotton, vegetable and other crops.
Compound C is Pyriproxyfen having chemical name as 2-[1-(4-phenoxyphenoxy) propan-2-yloxy] pyridine is a juvenile hormone analogue. Further, it is an insect growth regulator that can disrupt the growth of insects and possess the characteristics of activity, low toxicity, long-lasting effect, safety to crops, and little impact on the ecological environment.


Compound C has a good control effect on bemisia tabaci, scale insects, diamondback moth, spodoptera exigua, spodoptera litura, pear woodhopper, thrips, cotton bollworm and can be widely considered in hemiptera, thysanoptera, lepidoptera pest control, whitefly, scale insects, thrips, diamondback moth and other kind of small pests control effect is excellent.

Further, the insecticidal composition provides broad spectrum synergistic control i.e. grams of active ingredients required are less to achieve the desired level of control of insects-pests (caterpillars, sucking pets) and mites with one shot application. Moreover, it provides residual control i.e. longer duration of control with immediate crop protection and showcases the delay in development of resistance and effective control of hard to kill and resistant insect-pests and mites.

Furthermore, the insecticidal composition increases yield of treated plants (cereals, pulses, oilseeds, fiber crop, sugar crops, leafy vegetables, tuber crops, fruit crops, flowers, ornamentals, etc). The insecticidal composition increases yield due to greater number of tillers, more branches and sub branches, flowers and fruits as well as increases plant vigor and tolerance against insect-pests and mites damage.

The present invention optionally comprises agrochemically acceptable excipients and hence, those skilled in the art will appreciate that it is possible to utilize additional agrochemically acceptable excipients without departing from the scope of the present invention. The agrochemically acceptable excipient can be in the range from 0.1% to 99% of the total weight of the composition.

The amount of a composition according to the invention to be applied, will depend on various factors, such as the subject of the treatment, such as, for example plants, soil or seeds; the type of treatment, such as, for example spraying, dusting or seed dressing; the purpose of the treatment, such as, for example prophylactic or therapeutic disease control; or the application time. This amount of the combinations of the present invention to be applied can be readily deduced by a skilled agronomist.
The combination of the present invention is formulated in a manner which suits the specific application. The synergistic insecticidal composition of the present invention can be formulated as dry formulation or liquid formulation. Dry formulation includes Dustable powder (DP), Water soluble powder (SP), Wettable powder (WP), Wettable powder packed in water soluble pouch, Water dispersible granules (WDG/WG), Water soluble granule (SG), Emulsifiable granule (EG), Tablets (water soluble/dispersible), Oil dispersible powder (OP). The liquid formulations includes Capsule suspension (CS), Dispersible concentrate (DC), Emulsifiable concentrate (EC), Emulsion water-in-oil (EO), Emulsion oil-in-water (EW), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Suspension concentrate (SC), Suspo-emulsion (SE), Soluble concentrate (SL), Spreading oil (SO), Ultra-low volume (ULV) suspension, Ultra-low volume (ULV) liquid, Zeon Concentrate (ZC)-mixed formulation of CS and SC, Zoldine (ZE)-mixed formulation of CS and SE, ZW-mixed formulation of CS and EW.

More particularly, the composition is selected from Suspo Emulsion (SE), Suspension Concentrate (SC), Oil dispersion (OD), Micro Emulsion (ME) and Emulsifiable Concentrate (EC).

The inactive excipients used in above mentioned formulations are as follows:

Wetting agent: Ethylene oxide/propylene oxide (EO/PO) block copolymer, poly aryl phenyl ether phosphate, polyalkoxylated butyl ether, ethoxylated fatty alcohol, sodium dioctyl sulfosuccinate, sodium lauryl sulfate, sodium dodecyl benzene sulfonate, alkyl diphenyl sulfonates, sodium isopropyl naphthalene sulfonate, alkyl naphthalene sulfonate. Ethoxylated alcohol includes natural fatty alcohol (lauryl alcohol ethoxylate, lauryl alcohol alkoxylate), synthetic alcohol ethoxylate, tridecyl alcohol ethoxylate, 2-ethyl hexanol, 2-propylheptanol, isodecyl alcohol, trisiloxane ethoxylate, heptamethyl trisiloxane, modified form includes polyalkyleneoxide modified heptamethyl trisiloxane, polyether modified polysiloxane, polyalkyleneoxide modified trisiloxane, polyalkyleneoxide modified polydimethylsiloxane and mixture thereof.

Dispersing agent: Butyl polyalkylene oxide block copolymer, sodium naphthalene sulphonate formaldehyde condensates, alkylated naphthalene sulfonate, sodium salt, sodium salt of naphthalene sulfonate condensate, sodium ligno sulfonate, sodium polycarboxylate, ethylene-propylene oxide block copolymer, phenol sulfonate, sodium methyl oleoyl taurate, styrene acrylic acid copolymer, propylene oxide-ethylene oxide-copolymer, polyethylene glycol 2,4,6-tristyrylphenyl ether, ethoxylated tristrylphenol, tristyrylphenol-polyglycolether-phosphate, tristyrylphenole with 16 moles EO, oleyl-polyglycol ether with ethylene oxide, tallow fatty amine polyethylene oxide, nonylphenol polyglycol ether with 9-10 moles ethylene oxide, dodecyl benzene sulfonate in methylated and ethoxylated seed oil and mixture thereof.

Suspending agent: Aluminum magnesium silicate, bentonite clay, silica, attapulgite clay and mixture thereof.

Antifoaming agent: Silicone antifoam emulsion, dimethyl siloxane, polydimethyl siloxane, vegetable oil based antifoam, tallow based fatty acids and mixture thereof.

Anti-freezing agent: Ethylene glycol, propane diols, glycerin, urea, glycol, monoethylene glycol, diethylene glycol, 1,2-propylene glycol, polypropylene glycol, polyethylene glycol, magnesium sulfate heptahydrate, sodium chloride and mixture thereof.

Preservatives: 1,2-benzisothiazolin-3(2H)-one, sodium salt, sodium benzoate, 2-bromo-2-nitropropane-1,3-diol, formaldehyde, sodium o-phenyl phenate, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one and mixture thereof.

Thickeners: Xanthan gum, polyvinylcarbazole (PVK), carboxymethyl celluloses, polyvinyl alcohols, gelatin, sodium carboxymethylcellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, acacia gum and mixture thereof.

Stabilizers/Rheology modifier: Precipitated silica, clay and mixture thereof.

Emulsifier: Salts of dodecylbenzene sulphonate, Ca-salts or amine salts, sulphonates of other C11-C16 alkylbenzenes, calcium salt of alkylbenzene sulfonate, alkylether sulphates, alkylphenoletherphosphates, ester phosphates; non-ionic surfactants such as alkoxylated alcohols and alkylphenols, ethoxylated fatty acids, ethoxylated vegetable oils, ethoxylated castor oil, fatty acid esters, sorbitol and their ethoxylated derivatives, ethoxylated amines and condensates of glycerol; catanionic emulsifiers such as a cationic amine, optionally in combination with an alkylsulphonate or ether sulphonate or ether phosphate, alkoxylated alcohols, alkoxylated alkylphenols, ethoxylated fatty acids, ethoxylated vegetable oils, ethoxylated tristyrylphenol, tristyrlphenol with 16 moles EO, tristyrylphenol-polyglycolether-phosphate, fatty acid esters of sorbitol and ethoxylated derivatives; ethoxylated amines and condensates of glycerol; sulfonated alkylbenzenes in the range of C11-C16 and salts thereof; alkylether sulphates; alkyletherphosphates; alkylphenoletherphosphates; salts of phosphate esters of ethoxylated tristyrylphenol; salts of sulphated ethers of ethoxylated tristyrylphenol or a catanionic system, wherein a cationic amine is present in combination with an alkylsulphonate, an alkylethersulphonate, an ether sulphate, or an ether phosphate such as an alkyletherphosphate, nonylphenol polyethoxy ethanols, castor oil polyglycol ethers, polyadducts of ethylene oxide and polypropylene, tributyl phenoxy polyethoxy ethanol, octyl phenoxy polyethoxy ethanol, polyarylphenyl anionic ether sulfate, ammonium salt, octylphenol ethoxylate, polyoxyethylene sorbitan monooleate, ricinoleic acid ethoxylate and mixture thereof.

Solvent/Co-solvent: Diluent water, water, water soluble alcohols, dihydroxy alcohol ethers, methyl ester of oleic acid, isobutanol, tetrahydrofurfuryl alcohol, lower alcohol (1-4 carbon atoms) includes -methanol, ethanol, n-propanol, isopropanol, n-butanol, tert-butanol. Macromolecular alcohol includes polyethylene glycol, sorbitol, glucitol, dihydroxy alcohol ethers includes dihydroxy alcohol alkyl ether or dihydroxy alcohol aryl ethers. The examples of dihydroxy alcohol alkyl ether include ethylene glycol methyl ether, diethylene glycol methyl ether, propylene glycol methyl ether, di-propylene glycol methyl ether, ethylene glycol ethyl ether, diethylene glycol ethyl ether, propylene glycol ethyl ether, di-propylene glycol ethyl ether. The examples of dihydroxy alcohol aryl ethers include ethylene glycol phenyl ether, 5 diethylene glycol phenyl ether, propylene glycol phenyl ether, di-propylene glycol phenyl ether and mixture thereof.

Hyrdocarbons includes n-pentane, hexane, cyclohexane, methylcyclohexane, heptane, isooctane, benzene, toluene, xylene, isophorone and ester solvents such as methyloleate, dimethylamide and morpholineamide derivatives of C6-C16 fatty acids, and mono-alkylene carbonates such as ethylene carbonate, propylene carbonate and butylene carbonates, dimethylsulfoxide (DMSO), 2-ethylhexanol, n-butanol, n-alkylpyrrolidones, fatty acid dimethyl esters, fatty acid esters, dibasic esters, aromatic hydrocarbons, aliphatic hydrocarbons, one or more dimethylamides, such as C8-dimethylamide, C10-dimethylamide, C12-dimethylamide, ethylene glycol, propylene glycol, polyalkylene glycols, aromatic hydrocarbons, N-methyl-2-pyrrolidinone (NMP); dimethylformamide (DMF); dimethylisosorbide (DMI); isophorone; acetophenone; 1,3-dimethyl-2-imidazolidonone; lactate esters; dimethyl and diethylcarbonates; alcohols including methanol; ethanol; iso-propanol; n-propanol; n-butanol; iso-butanol; tert-butanol; methyl L-lactate, 2-ethylhexyl L-lactate, ethyl L-lactate, n-butyl L-lactate, gamma butyrolactone, propylene carbonate, solvent naphtha and mixture thereof.

Solvent for the present formulation are also selected from vegetable oil (plant, seed or tree) or it's alkylated or ethoxylated or esterified. The alkylated vegetable oil may be methylated vegetable oil or ethylated vegetable oil. The vegetable oils include olive oil, kapok oil, castor oil, papaya oil, camellia oil, sesame oil, corn oil, rice bran oil, cotton seed oil, soybean oil, groundnut oil, rapeseed-mustard oil, linseed oil, tung oil, sunflower oil, safflower oil, coconut oil. The alkyl ester of vegetable oils includes methyl ester, ethyl ester, propyl ester or butyl ester of vegetable oils. Some of the examples are methylated seed oil, polyalkyleneoxide modified polydimethylsiloxane alkylphenol ethoxylate, oleic acid methyl ester, rapeseed oil methyl ester, rapeseed oil ethyl ester, rapeseed oil propyl esters, rapeseed oil butyl esters, soybean oil methyl ester, soybean oil ethyl ester, soybean oil propyl ester, soybean oil butyl ester, castor oil methyl ester, castor oil ethyl ester, castor oil propyl ester, castor oil butyl ester, cotton seed oil methyl ester, cotton seed oil ethyl ester, cotton seed oil butyl ester, cotton seed oil propyl ester, tall oil fatty acids esters-tallow methyl ester, tallow ethyl ester, tallow propyl ester, bio-diesel, mineral oil (aromatic solvents, isoparaffin, base solvent), fatty acid amides (C1-C3 amines, alkylamines or alkanolamines with C6-C18 carboxylic acids), fatty acids, alkyl esters of fatty acids, methyl and ethyl oleate, methyl and ethyl soyate, alkyl benzenes and alkylnaphthalenes, polyalkylene glycol ethers, fatty acid diesters, fatty alkylamides and diamides, dialkylene carbonates, ketones, alcohols, cyclohexanone, acetophenone, dimethyl sulfoxide, benzyl alcohol, butanol, N-octanol, N-propanol, 2-ethyl hexanol, tetrahydro furfuryl alcohol, isophorone, fatty acid dimethyl amide, 2-hexylethyl lactate, propylene carbonate and mixture thereof.

EXAMPLES

The present invention has been described with reference to specific embodiment which is merely illustrative and not intended to limit the scope of the invention as defined in the present complete specification.


BIOLOGICAL EXAMPLES:

The synergistic pesticide action of the inventive mixtures can be demonstrated by the experiments below. A synergistic effect exists wherever the action of a combination (ready-mix) or tank mix of active ingredient is greater than the sum of the action of each of the components alone. Therefore, a synergistically effective amount or an effective amount of a synergistic composition or combination is an amount that exhibits greater pesticide activity than the sum of the pesticide activities of the individual components.

In the field of agriculture, it is often understood that the term "synergy" is as defined by Colby S.R. in an article entitled "Calculation of the synergistic and antagonistic responses of herbicide combinations" published in the journal Weeds, 1967, 15, p.20-22, incorporated herein by reference in its entirety. The action expected for a given combination of two or three active components can be calculated as follows:



The objective of the present studies was to study the synergism and benefits. The compositions comprising of Compound A + Compound B + Compound C were evaluated for its bio efficacy in different crops.

EXAMPLE 1: CONTROL OF INSECT-PESTS IN CHILLY CROP
Crop : Chilly
Number of Treatments: Eight including untreated check (UTC).
Plot size : 7 m x 6 m.
Crop stage : 65 days after transplanting.
Method of application: Foliar spray with battery operated backpack sprayer.
Water volume : 460 liter per hectare

Observation Methods:
Thrips (Scirtothrips dorsalis) control (%): Thrips are minute, slender insects with fringed wings and yellowish color. It mainly feeds on buds and young leaves. Its population counted by shaking twigs gently over black color piece of paper. Record the observations from four twigs per plant and 10 plants per plot. 20 hills per plot. The data on average number of live thrips per plant calculated and thrips control (as observed control) calculated by below given formula. Apply Colby formula to calculate expected control and thereby synergism.



Mite (Polyphagotarsonemus latus) control (%): The infestation of chilly mite causes downward curling and crinkling of leaves; leaves with elongated petiole; stunted growth of plant. Record the motile stages of mite with pocket microscope from five spots per leaf, ten leaves per plant. Record the observations from ten plants per plot.



SE formulation Range Actual
Description content (% w/w) -
Compound A technical 2 to 10 3.21
Compound B technical 15 to 35 25
Compound C technical 3 to 10 6.32
Emulsifier 8 to 20 12.72
Wetting agent 2 to 10 5.00
Dispersing agent 3 to 10 5.51
Suspending agent 0.1 to 2 0.50
Preservative 0.1 to 2 0.18
Anti freezing agent 1 to 10 3.90
Rheology modifier/stabilizer 0.1 to 2 0.50
Anti foaming agent 0.1 to 2 0.48
Thickener 0.1 to 2 0.15
Solvent q.s 36.53


Composition of Compound A 3.21%+Compound B 25%+Compound C 6.32% SE (Suspo Emulsion) [T1 of Ex.1]

Ingredients Description content (% w/w)
Compound A technical (93.5% purity) Active Ingredient 3.21
Compound B technical (96% purity) Active Ingredient 25
Compound C technical (95% purity) Active Ingredient 6.32
Polyarylphenyl anionic ether sulfate, ammonium salt Emulsifier 2.62
Octylphenol ethoxylate Emulsifier 10.10
Polyalkyleneoxide modified trisiloxane Wetting agent 5.00
Ethoxylated tristrylphenol Dispersing agent 4.00
Butyl Polyalkylene Oxide block copolymer Dispersing agent 1.51
Aluminium magnesium silicate Suspending agent 0.50
1,2-benzisothiazolin-3(2H)-one Preservative 0.18
1,2-propylene glycol Anti freezing agent 3.90
Precipitated Silica Rheology modifier/ stabilizer 0.50
Polydimethylsiloxane Anti foaming agent 0.48
Xanthan gum Thickener 0.15
Diluent water Solvent/Co-solvent 36.53
Total 100.00


Storage Stability: Compound A 3.21%+Compound B 25%+Compound C 6.32% SE (Suspo Emulsion) [T1 of Ex.1]
Laboratory storage stability for 14 days
Parameters Specification Initial At 54±2 0C At 0±2 0C
Compound A content ( %, w/w) 3.05 to 3.53 3.10 3.09 3.10
Compound B content (%, w/w) 22.80 to 25.20 24.31 24.29 24.31
Compound C content (%, w/w) 5.70 to 6.60 6.15 6.14 6.15
Compound A suspensibility (%) 80 95.43 95.41 95.43
Compound B suspensibility (%) 80 96.78 96.74 96.78
Compound C suspensibility (%) 80 98.99 98.92 98.99
pH range (1% aq. Suspension) 5.5 to 8.0 6.34 6.31 6.34
Pourability (%) 95 Min. 99.45 99.44 99.45
Specific gravity 1.05-1.10 1.08 1.08 1.08
Viscosity at spindle no.62,20 rpm 350-800 cps 540 545 542
Particle size (micron) D50<3, D90<10 2.1,5.7 2.1,5.8 2.2,5.7
Persistent foam ml (after 1 minute) max. 60 Nil Nil Nil


Room temperature storage stability up to 12 months [T1 of Ex.1]
Parameters Specification 1 month 6 month 12 month
Compound A content ( %, w/w) 3.05 to 3.53 3.10 3.09 3.07
Compound B content (%, w/w) 22.80 to 25.20 24.31 24.30 24.28
Compound C content (%, w/w) 5.70 to 6.60 6.15 6.13 6.11
Compound A suspensibility (%) 80 95.43 95.42 95.39
Compound B suspensibility (%) 80 96.78 96.76 96.71
Compound C suspensibility (%) 80 98.99 98.96 98.93
pH range (1% aq. Suspension) 5.5 to 8.0 6.34 6.35 6.37
Pourability (%) 95 Min. 99.45 99.44 99.44
Specific gravity 1.05-1.10 1.08 1.08 1.08
Viscosity at spindle no. 62, 20 rpm 350-800 cps 540 542 545
Particle size (micron) D50<3, D90<10 2.1,5.7 2.1,5.7 2.1,5.7
Persistent foam in ml (after 1 minute) max. 60 Nil Nil Nil
The composition of Compound A 3.21% + Compound B 25% + Compound C 6.32% SE (Suspo Emulsion) meets the all inhouse specifications for storage stability studies in laboratory (at 54±2 °C and at 0±2 °C for 14 days) and room temperature (for 12 months).


Manufacturing process for 100 kg batch of Compound A 3.21%+Compound B 25%+Compound C 6.32% SE (Suspo Emulsion) [T1 of Ex.1]
Step 1 Preparation of 2% Gum Solution: Xanthan gum (2.0 kg) and 1,2-benzisothiazoline-3-one (2.0 kg) was charged into 96.0 kg water and homogenized. It was prepared 12 to 18 hours prior to use.
Step 2 EC premix: Octylphenol ethoxylate (10.10 kg) was added into other vessel containing slow stirring. Further, polyarylphenyl anionic ether sulfate, ammonium salt (2.62 kg) and Compound C technical (6.32 kg) were added and mixed properly for 30 to 45 minutes.
Step 3 DM water (30 kg) and 1,2-propylene glycol (3.9 kg) were charged into designated vessel and mixed thoroughly.
Step 4 Aluminum magnesium silicate (0.5 kg), ethoxylated tristrylphenol (4 kg), butyl polyalkylene oxide block copolymer (1.51 kg) and polydimethylsiloxane (0.16 kg) were added into the vessel containing water and the contents were homogenised for 45 to 60 minutes using high shear homogenizer.
Step 5 Compound A technical (3.21 kg) and Compound B technical (25 kg) were added under stirring and homogenized well.
Step 6 Prior grinding half of the quantity of polydimethylsiloxane (0.15 kg) was added and it was subjected to grinding in dyno mill till desired particle size was achieved.
Step 7 The remaining polydimethyl siloxane (0.16 kg) antifoam was added after completion of grinding process and prior sampling for in process analysis.
Step 8 The EC premix was mixed to this milled slurry under slow stirring and homogenized for 30 to 45 minutes
Step 9 6.35 kg of 2% Xanthum gum solution and 5.0 kg of polyalkyleneoxide modified trisiloxane were added to this formulation and homogenized for 30 minutes.
Step 10 The final formulation was sent to QC for quality check.


TABLE 1: TREATMENT DETAILS
S. No. Treatment details Dose per hectare
Formulation (ml) Active ingredients (g)
T1 Compound A 3.21%+Compound B 25%+Compound C 6.32% SE 1000 ml 30+240+60
T2 Compound B 24%+Compound C 6% SE 1000 ml 240+60
T3 Compound A 5%+Compound C 10% EC 600 ml 30+60
T4 Compound A 5%+Compound B 40% SC 600 ml 30+240
T5 Compound A 10% EC 300 ml 30
T6 Compound B 47.8% SC 502 ml 240
T7 Compound C 10% EC 600 ml 60
T8 Untreated Check (UTC) - -

T1: present composition, T2 to T4: known mixtures, T5 to T7 are single compounds and T8 is UTC.

TABLE 2: CONTROL OF THRIPS AND MITES IN CHILLY CROP

S.No Thrips control (%) Mite control (%)
3 DAA 10 DAA 3 DAA 10 DAA
Observed Expected Colby's ratio Observed Expected Colby's ratio Observed Expected Colby's ratio Observed Expected Colby's ratio
T1 100.00 89.36 1.12 98.97 75.29 1.31 99.12 90.84 1.09 96.82 83.08 1.17
T2 69.32 68.87 1.01 44.39 46.81 0.95 76.95 75.79 1.02 61.28 64.32 0.95
T3 75.95 74.32 1.02 57.86 58.58 0.99 74.12 72.29 1.03 57.84 59.87 0.97
T4 87.15 85.84 1.02 71.98 72.30 1.00 88.36 87.50 1.01 78.27 80.00 0.98
T5 65.82 - - 53.55 - - 62.18 - - 52.58 - -
T6 58.57 - - 40.36 - - 66.96 - - 57.83 - -
T7 24.86 - - 10.82 - - 26.73 - - 15.38 - -
T8 0.00 - - 0.00 - - 0.00 - - 0.00 - -

Abbreviation: DAA: Days after application.

The treatment of Compound A 3.21% + Compound B 25% + Compound C 6.32% SE provides synergistic control of thrips and mites on 3rd and 10th DAA (Days after application).

Conclusion [Ex.1]: Among the various compositions as shown in Table 1 treatment number T1 is considered to be present inventive composition which showed excellent synergism and effectiveness against thrips control at 3DAA [days after application] for 100% and at 10 DAA it showed 98.97% of control as compared to the known mixtures. Moreover, the mite control at 3DAA showed 99.12% control and at 10 DAA it showed 96.82% of control as compared to the known mixtures. Furthermore, the colby's ratio of the present invention is more than 1 which shows stronger and effective synergism in chilly crop.

EXAMPLE 2: CONTROL OF INSECT-PESTS IN FRENCH BEAN CROP
Crop : French bean
Number of Treatments: Eight including untreated check (UTC).
Plot size : 8 m x 5 m.
Crop stage : 75 days after transplanting.
Method of application: Foliar spray with battery operated backpack sprayer.
Water volume : 500 liter per hectare

Observation Methods:
Jassid (Amrasca biguttula biguttula) control (%): Jassids are small, soft bodied insects, walk diagonally and suck the cell sap by remaining on the lower surface of leaf. Count the number of live insects per trifoliate leaf, three leaves per plant and ten plants per plot. The data on average number of live jassid per plant calculated and jassid control (as observed control) calculated.

Pod borer (Helicoverpa armigera) larval control (%): Count the number of live larvae per plant, ten plants per plot and calculate the larval control.



SC formulation Range Actual
Description content (% w/w) -
Compound A technical 2 to 10 4.28
Compound B technical 15 to 35 31.25
Compound C technical 3 to 10 7.37
Wetting agent 2 to 10 5.00
Dispersing agent 3 to 10 5.14
Suspending agent 0.1 to 2 0.52
Anti foaming agent 0.1 to 2 0.28
Preservative 0.1 to 2 0.17
Anti freezing agent 1 to 10 5.75
Thickener 0.1 to 2 0.12
Solvent/Co-solvent q.s. 40.12


Composition of Compound A 4.28%+Compound B 31.25%+Compound C 7.37% SC (Suspension Concentrate) [T1 of Ex.2]
Ingredients Description content (% w/w)
Compound A technical (93.5% purity) Active Ingredient 4.28
Compound B technical (96% purity) Active Ingredient 31.25
Compound C technical (95% purity) Active Ingredient 7.37
Polyalkyleneoxide modified trisiloxane Wetting agent 5.00
Ethylene-propylene oxide block copolymer Dispersing agent 3.85
Sodium naphthalene sulphonate formaldehyde condensates Dispersing agent 1.29
Aluminum magnesium silicate Suspending agent 0.52
Polydimethylsiloxane Anti foaming agent 0.28
1,2-benzisothiazolin-3(2H)-one Preservative 0.17
1,2-Propylene glycol Anti freezing agent 5.75
Xanthan gum Thickener 0.12
Diluent water Solvent/Co-solvent 40.12
Total 100.00


Storage Stability: Compound A 4.28%+Compound B 31.25%+Compound C 7.37% SC (Suspension Concentrate) [T1 of Ex.2]
Laboratory storage stability for 14 days
Parameters Specification Initial At 54±2 0C At 0±2 0C
Compound A content ( %, w/w) 3.80 to 4.40 4.12 4.11 4.12
Compound B content (%, w/w) 28.50 to 31.50 30.32 30.31 30.32
Compound C content (%, w/w) 6.65 to 7.70 7.19 7.18 7.19
Compound A suspensibility (%) 80 92.79 92.75 92.79
Compound B suspensibility (%) 80 94.45 94.43 94.45
Compound C suspensibility (%) 80 91.78 91.77 91.78
pH range (1% aq. Suspension) 5.5 to 8.0 6.70 6.71 6.70
Pourability (%) 95 98.00 97.98 97.97
Specific gravity 1.05-1.15 1.07 1.07 1.07
Viscosity at spindle no.62,20 rpm 350-800 cps 510 517 515
Particle size (micron) D50<3, D90<10 1.9,6.2 1.9,6.3 1.9,6.2
Persistent foam ml (after 1 minute) max. 60 Nil Nil Nil


Room temperature storage stability up to 12 months [T1 of Ex.2]
Parameters Specification 1 month 6 month 12 month
Compound A content ( %, w/w) 3.80 to 4.40 4.12 4.11 4.09
Compound B content (%, w/w) 28.50 to 31.50 30.32 30.31 30.29
Compound C content (%, w/w) 6.65 to 7.70 7.19 7.17 7.15
Compound A suspensibility (%) 80 92.79 92.77 92.56
Compound B suspensibility (%) 80 94.45 94.39 94.24
Compound C suspensibility (%) 80 91.78 91.72 91.65
pH range (1% aq. Suspension) 5.5 to 8.0 6.70 6.71 6.75
Pourability (%) 95 98.00 97.79 97.71
Specific gravity 1.05-1.15 1.07 1.07 1.07
Viscosity at spindle no. 62, 20 rpm 350-800 cps 510 515 519
Particle size (micron) D50<3, D90<10 1.9,6.2 2.0,6.3 2.0,6.5
Persistent foam in ml (after 1 minute) max. 60 Nil Nil Nil
The composition of Compound A 4.28%+Compound B 31.25%+Compound C 7.37% SC (Suspension Concentrate) meets the all inhouse specifications for storage stability studies in laboratory (at 54±2 °C and at 0±2 °C for 14 days) and room temperature (for 12 months).


Manufacturing process for 100 kg batch of Compound A 4.28% + Compound B 31.25% + Compound C 7.37% SC (Suspension Concentrate) [T1 of Ex.2]
Step 1 Preparation of 2% Gum Solution: Xanthan gum (2.0 kg) and 1,2-benzisothiazoline-3-one (2.0 kg) were charged into 96.0 kg water and homogenized. The said solution was made 12 to 18 hour prior to use.
Step 2 DM water (34.12 kg) and 1,2-Propylene glycol (5.75 kg) were charged into designated vessel and mixed thoroughly.
Step 3 Ethylene-propylene oxide block copolymer (3.85 kg), Sodium naphthalene sulphonate formaldehyde condensates (1.29 kg) and Aluminium magnesium silicate (0.52 kg) were added into the vessel containing water and the contents were homogenised for 45 to 60 minutes using high shear homogenizer.
Step 4 Compound A technical (4.28 kg), Compound B technical (31.25 kg) and Compound C technical (7.37 kg) were slowly added to this premix and homogenised to achieve uniform slurry ready for grinding.
Step 5 Prior grinding half the quantity of Polydimethylsiloxane (0.14 kg) was added and then the material was subjected to grinding in Dyno mill till desired particle size was achieved.
Step 6 Remaining Polydimethyl siloxane (0.14 kg) antifoam was added after completion of grinding process and prior the sampling for in process analysis.
Step 7 6.0 kg of 2% Xanthum gum solution and 5.0 kg of polyalkyleneoxide modified trisiloxane were added to this formulation and homogenized for 30 minutes.
Step 8 The final formulation was sent to QC for quality check.



TABLE 3: TREATMENT DETAILS
S.No. Treatment details Dose per hectare
Formulation (ml) Active ingredients (g)
T1 Compound A 4.28%+Compound B 31.25%+Compound C 7.37% SC 875 ml 35+262.5+61.25
T2 Compound B 30%+Compound C 7% SE 875 ml 262.5+61.25
T3 Compound A 4%+Compound C 7% EC 875 ml 35+61.25
T4 Compound A 5%+Compound B 37.5% SC 700 ml 35+262.5
T5 Compound A 10% EC 350 ml 35
T6 Compound B 50% WP 525 g 262.5
T7 Compound C 10% EC 612.5 ml 61.25
T8 Untreated Check (UTC) - -

T1: present composition, T2 to T4: known mixtures and T5 to T7 are single compounds and T8 is UTC.

TABLE 4: CONTROL OF INSECT-PESTS IN FRENCH BEAN

sr.no. Jassid control (%) Pod borer larval control (%)
3 DAA 10 DAA 3 DAA 10 DAA
Observed Expected Colby's ratio Observed Expected Colby's ratio Observed Expected Colby's ratio Observed Expected Colby's ratio
T1 100.00 92.15 1.09 98.89 82.64 1.20 96.88 85.84 1.13 89.58 74.12 1.21
T2 74.16 73.70 1.01 48.83 53.29 0.92 42.86 44.94 0.95 26.84 29.15 0.92
T3 80.87 78.33 1.03 67.28 68.28 0.99 79.86 79.06 1.01 65.28 67.46 0.97
T4 91.28 89.18 1.02 77.73 79.67 0.98 83.12 82.61 1.01 68.35 70.94 0.96
T5 70.15 - - 62.84 - - 74.28 - - 63.47 - -
T6 63.76 - - 45.28 - - 32.38 - - 20.46 - -
T7 27.42 - - 14.63 - - 18.57 - - 10.93 - -
T8 0.00 - - 0.00 - - 0.00 - - 0.00 - -

The treatment of Compound A 4.28%+Compound B 31.25%+Compound C 7.37% SC (T1) provides synergistic control of jassid and pod borer larvae on 3rd and 10th DAA (Days after application).

Conclusion [Ex.2]: Among the various compositions as shown in Table 3, T1 is the present composition which showed excellent synergism and effectiveness against Jassid control and pod borer larval control at 3 DAA and 10 DAA. In particular, at 3 DAA [days after application] 100% jassid control is observed whereas at 10 DAA 98.89% of jassid control is observed as compared to other known mixtures. Further, at 3 DAA treatment number T1 showed 96.88% of pod borer larval control likewise at 10 DAA 89.58% of control is observed when compared to other known mixtures. At last the colby's ratio observed is more than 1 which means stronger synergism in French bean crop.

EXAMPLE 3: RESIDUAL CONTROL INSECT-PESTS IN OKRA CROP
Crop : Okra
Number of Treatments: Nine
Plot size : 10m x 5m.
Crop stage : 75 days after transplanting.
Method of application: Foliar spray with battery operated backpack sprayer.
Water volume : 500 liter per hectare

Observation Methods:
Whitefly (Bemisia tabaci) control (%): Count the number of live insects per leaf, three leaves per plant and ten plants per plot. The data on average number of live whiteflies per plant calculated and whitefly control (as observed control) calculated.

Jassid (Amrasca biguttula biguttula) control (%): Jassids are small, soft bodied insects, walk diagonally and suck the cell sap by remaining on the lower surface of leaf. Count the number of live insects per trifoliate leaf, three leaves per plant and ten plants per plot. The data on average number of live jassid per plant calculated and jassid control (as observed control) calculated.

Fruit borer larval control (%): Count the number of live larvae per plant, ten plants per plot. Calculate larval control.



TABLE 5: TREATMENT DETAILS
S. No. Treatments Dose per hectare
Active Ingredients (g) Formulation (ml)
T1 Compound A 2.5%+Compound B 25%+Compound C 7% SE 20+200+56 800 ml
T2 Compound B 18%+Compound C 8%+Dinotefuran 5% SC 180+80+50 1000 ml
T3 Compound B 30%+Compound C 8% SE 300+80 1000 ml
T4 Compound A 2.25%+Cyantraniliprole 4.5%+Compound C 6% SC 22.5+45+60 1000 ml
T5 Compound A 2.25%+Chlorantraniliprole 2.25%+Compound C 6% SC 22.5+22.5+60 1000 ml
T6 Compound A 2%+Chlorantraniliprole 2.5%+Compound B 20% SC 20+25+200 1000 ml
T7 Compound A 2%+Cyantraniliprole 4.5%+Compound B 20% SC 20+45+200 1000 ml
T8 Compound A 4.5%+Compound C 9.5%+Flonicamid 13% SE 33.75+71.25+97.5 750 ml
T9 Control Plot (water spray only) - -

T1: present composition, T2 to T8: known compositions/prior arts and T9 is control.

TABLE 6A: RESIDUAL CONTROL
S.No. Whitefly control (%) Jassid control (%)
3 DAA 7 DAA 10 DAA 14 DAA 3 DAA 7 DAA 10 DAA 14 DAA
T1 97.58 95.84 91.58 87.86 98.68 93.28 88.45 84.63
T2 90.36 85.26 73.56 65.32 90.35 83.58 77.43 60.48
T3 95.47 92.64 83.32 74.28 86.38 78.73 65.62 57.27
T4 93.86 89.26 78.45 70.84 91.42 83.58 75.67 65.36
T5 92.85 88.78 76.92 67.42 90.28 80.24 68.42 58.63
T6 95.45 91.42 81.43 70.85 93.46 84.27 77.15 69.28
T7 96.12 92.14 84.56 72.44 95.32 88.81 82.56 76.98
T8 88.14 82.63 69.54 54.32 96.38 91.58 85.17 80.35
T9 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

The field trial results shows that present composition T1: Compound A 2.5% + Compound B 25% + Compound C 7% SE provides desirable residual control of whitefly and jassid for more than 14 days showcasing the highest number of control on whitefly and jassid respectively as compared to all known/prior art compositions (T2 to T8).

TABLE 6B: RESIDUAL CONTROL AND FRUIT YIELD
S.No. Fruit borer control (%) Number of healthy fruits per 5 plants Increase in healthy fruits over control plot
3 DAA 7 DAA 10 DAA 14 DAA
T1 100.00 96.94 92.42 88.78 43.30 37.46
T2 46.28 30.84 17.32 6.83 36.40 15.56
T3 54.82 40.72 23.18 10.78 37.60 19.37
T4 100.00 95.72 91.24 85.28 39.60 25.71
T5 100.00 96.53 92.18 88.58 40.90 29.84
T6 100.00 98.25 94.84 90.21 41.20 30.79
T7 100.00 94.92 90.78 82.43 39.10 24.13
T8 100.00 94.28 88.67 80.16 38.80 23.17
T9 0.00 0.00 0.00 0.00 31.50 0.00

The present composition T1: Compound A 2.5% + Compound B 25% + Compound C 7% SE also provides excellent desirable residual control of fruit borer larvae and also yielded higher number of healthy fruits as compared to all known/prior art compositions (T2 to T8).

Conclusion [Ex.3]: Among the various compositions as shown in Table 5, T1 is the present composition which shows phenomenal results in terms of whitefly control, jassid control, fruit borer control and number of healthy fruits per 5 plants. At first, the whitefly control at 3 DAA [days after application] is observed at 97.58%, at 7 DAA it is at 95.84%, at 10 DAA it is at 91.58% and at 14 DAA it is at 87.86%. Moreover, jassid control at 3 DAA shows 98.68%, 93.28% control at 7 DAA, at 10 DAA it shows 88.45% control and at 14 DAA it shows 84.63% of control as compared to the known compositions.

Moreover, the treatment number T1 at 3 DAA showed 100% fruit borer control followed by 96.94% at 7 DAA, 92.42% of control at 10 DAA and 88.78% control at 14 DAA as compared to the known compositions. Furthermore, the number of healthy fruits per 5 plants of T1 is 43.30 and increase in healthy fruits over the control plot is 37.46 when compared to the known compositions in okra crop.

EXAMPLE 4: RESIDUAL CONTROL INSECT-PESTS IN WATERMELON
Crop : Watermelon
Number of Treatments: Seven
Plot size : 4 rows of 5-meter length per treatment.
Crop stage : 58 days after sowing.
Method of application: Foliar spray with battery operated backpack sprayer.
Water volume : 400 liter per hectare

Observation Methods:
RED SPIDER MITE (TETRANYCHUS URTICAE) CONTROL (%): The infestation of mite causes downward curling and crinkling of leaves; leaves with elongated petiole; stunted growth of plant. Record the motile stages of mite with pocket microscope from five spots per leaf, ten leaves per plant. Record the observations from ten plants per plot.



JASSID (AMRASCA BIGUTTULA BIGUTTULA) CONTROL (%): Jassids are small, soft bodied insects, walk diagonally and suck the cell sap by remaining on the lower surface of leaf. Count the number of live insects per trifoliate leaf, three leaves per plant and ten plants per plot. The data on average number of live jassid per plant calculated and jassid control (as observed control) calculated.

EPILACHNA BEETLE CONTROL (%): Count the number of live beetles per leaf, three leaves per vine, and ten vines per plot. The data on average number of live beetles per plant calculated and beetle control (as observed control) calculated.

OD formulation Range Actual
Description content (% w/w) -
Compound A technical 2 to 10 -
Compound B technical 15 to 35 -
Compound C technical 3 to 10 -
Wetting agent 2 to 10 5.00
Dispersing agent 2 to 15 10.51
Emulsifier 4 to 10 6.70
Anti foaming agent 0.1 to 2 0.28
Preservative 0.1 to 2 0.17
Stabilizer/rheology modifier 1 to 10 1.10
Solvent/Co-solvent q.s. 39.80


Composition of Compound A 4.28% + Compound B 25% + Compound C 7.37% OD (Oil Dispersion) [T2 of Ex.4]
Ingredients Description content (% w/w)
Compound A technical (93.5% purity) Active Ingredient 4.28
Compound B technical (96% purity) Active Ingredient 25.00
Compound C technical (95% purity) Active Ingredient 7.37
Polyalkyleneoxide modified trisiloxane Wetting agent 5.00
Tristyrylphenol-polyglycolether-phosphate Dispersing agent 3.71
Dodecyl benzene sulfonate in methylated and ethoxylated seed oil Dispersing agent 6.80
Fatty acid ethoxylates Emulsifier 6.70
Precipitated silica Stabilizer/rheology modifier 1.10
Polydimethylsiloxane Antifoaming 0.12
1,2-benzisothiazolin-3(2H)-one Preservative 0.12
Methyl ester of Oleic acid Solvent/co-solvent 39.80
Total 100.00


Storage Stability: Compound A 4.28% + Compound B 25% + Compound C 7.37% OD (Oil Dispersion) [T2 of Ex.4]
Laboratory storage stability for 14 days
Parameters Specification Initial At 54±2 0C At 0±2 0C
Compound A content ( %, w/w) 3.80 to 4.40 4.17 4.16 4.17
Compound B content (%, w/w) 22.80 to 25.20 24.31 24.30 24.31
Compound C content (%, w/w) 6.65 to 7.70 7.21 7.20 7.21
Compound A suspensibility (%) 80 90.89 90.87 90.89
Compound B suspensibility (%) 80 92.34 92.30 92.34
Compound C suspensibility (%) 80 89.91 89.90 89.91
pH range (1% aq. Suspension) 5.5 to 8.0 6.10 6.20 6.10
Pourability (%) 95 99.00 98.79 99.00
Specific gravity 0.93-1.02 0.97 0.97 0.97
Viscosity at spindle no.62,20 rpm 150-500 cps 170 175 170
Particle size (micron) D50<3, D90<10 1.9,7.2 2.0,7.3 1.9,7.2
Persistent foam ml (after 1 minute) max. 60 Nil Nil Nil


Room temperature storage stability up to 12 months [T2 of Ex.4]
Parameters Specification 1 month 6 month 12 month
Compound A content (%, w/w) 3.80 to 4.40 4.17 4.16 4.13
Compound B content (%, w/w) 22.80 to 25.20 24.30 24.29 24.25
Compound C content (%, w/w) 6.65 to 7.70 7.21 7.20 7.16
Compound A suspensibility (%) 80 90.88 90.81 90.45
Compound B suspensibility (%) 80 92.34 92.29 91.98
Compound C suspensibility (%) 80 89.90 89.87 89.45
pH range (1% aq. Suspension) 5.5 to 8.0 6.10 6.11 6.12
Pourability (%) 95 99.00 98.89 98.78
Specific gravity 0.93-1.02 0.97 0.97 0.97
Viscosity at spindle no. 62, 20 rpm 150-500 cps 170 175 185
Particle size (micron) D50<3, D90<10 2.0,7.3 2.0,7.3 2.1,7.4
Persistent foam in ml (after 1 minute) max. 60 Nil Nil Nil
The composition of Compound A 4.28% + Compound B 25% + Compound C 7.37% OD (Oil Dispersion) meets the all inhouse specifications for storage stability studies in laboratory (at 54±2 °C and at 0±2 °C for 14 days) and room temperature (for 12 months).


Manufacturing process for 100 kg batch of Compound A 4.28 % + Compound B 25% + Compound C 7.37 % OD (Oil Dispersion) [T2 of Ex.4]
Step 1 OD Premix: 39.80 kg of Methyl ester of Oleic acid was charged into a designated vessel for OD production.
Step 2 3.71 kg of Tristyrylphenol-polyglycolether phosphate, 6.8 kg of Dodecyl benzene sulfonate in methylated and ethoxylated seed oil, 6.7 kg of Fatty acid ethoxylates, 0.12 kg of 1,2-benzisothiazolin-3(2H)-one and 1.1 kg of Precipitated silica were added and the contents were homogenised for 45 to 60 minutes using high shear homogeniser.
Step 3 Compound A technical (4.28 kg), Compound B technical (25.00 kg) and Compound C technical (7.37 kg) were slowly added to this premix and homogenised to achieve uniform slurry ready for grinding.
Step 4 Before grinding, half the quantity of Polydimethylsiloxane (0.06 kg) was added and then the material was subjected to grinding in Dyno mill till desired particle size is achieved.
Step 5 The remaining Polydimethylsiloxane (0.06 kg) and 5.00 kg of Polyalkyleneoxide modified trisiloxane were added to avoid foaming.
Step 6 The final formulation was sent to QC for quality check.


TABLE 7: TREATMENT DETAILS
S.No. Treatments Dose (g.a.i/ha)

T1 Compound A 8%+Compound B 15%+Compound C 8% OD 60+112.5+60
T2 Compound A 4.28%+Compound B 25%+Compound C 7.37% OD 30+180+52.5
T3 Compound A 2%+Compound B 28%+Compound C 4% OD 15+210+30
T4 Compound A 10% EC+Compound B 50% WP 75+300
T5 Compound A 10% EC+Compound C 10% EC 75+75
T6 Compound B 25%+Compound C 5% SE 312.5+62.5
T7 Control Plot (water spray only) -

T1 to T3: present compositions, T4 and T5: farm tank mixes at higher doses and T6: prior art.

TABLE 8: RESIDUAL CONTROL OF RED SPIDER MITE AND EPILACHNA BEETLE IN WATERMELON

S.No. Red spider mite control (%) Epilachna beetle control (%)
5 DAA 10 DAA 15 DAA 20 DAA 5 DAA 10 DAA 15 DAA 20 DAA
T1 96.83 93.84 90.48 88.48 100.00 100.00 97.48 93.28
T2 92.18 90.68 86.73 83.28 100.00 99.32 93.54 89.65
T3 91.52 87.49 83.55 80.91 97.49 93.28 87.48 81.43
T4 85.38 80.48 74.20 60.37 96.49 90.84 79.53 64.73
T5 82.15 76.52 70.53 55.43 91.28 84.85 68.38 47.73
T6 78.95 68.38 56.46 44.86 85.43 70.28 57.42 36.48
T7 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

All the present compositions (T1 to T3) provide excellent residual control of red spider mite and epilachna beetles in watermelon crop as compared to their on-farm tank mixes (T4, T5) and ready mix (T6) at higher doses. All present compositions shows excellent synergism in terms of level of control and duration of control.

Conclusion [Ex.4]: Among the various compositions as shown in Table 7, the present compositions are T1 to T3. The present compositions proved its effectiveness against Red spider mite control and Epilachna beetle control at 5 DAA, 10 DAA, 15 DAA and 20 DAA. In particular T1-T3 at 5 DAA showed 91.52% to 96.83% of control, at 10 DAA 87.49% to 93.84% of control, at 15 DAA 83.55% to 90.48% of control and at 20 DAA 80.91% to 88.48% of red spider mite control.

Further, in case of epilachna beetle control T1-T3 at 5 DAA showed 97.49% to 100% control, at 10 DAA93.28% to 100%, at 15 DAA 87.48% to 97.48% and at 20 DAA 81.43% to 93.28% of epilachna beetle control in watermelon crop as compared to farm tank mixes at higher dose and prior art composition.

The below shown table showcases Micro Emulsion (ME) and Emulsifiable Concentrate (EC) formulation:

ME formulation Range Actual
Description content (% w/w) -
Compound A technical 2 to 10 -
Compound B technical 15 to 35 -
Compound C technical 3 to 10 -
Wetting agent 2 to 10 5.00
Emulsifier 20 to 40 36.08
Solvent/Co-solvent 20 to 40 30.16


Composition of Compound A 2.67% + Compound B 20.83% + Compound C 5.26% ME (Micro Emulsion)

Ingredients Description content (% w/w)
Compound A technical (93.5% purity) Active Ingredient 2.67
Compound B technical (96% purity) Active Ingredient 20.83
Compound C technical (95% purity) Active Ingredient 5.26
Polyalkyleneoxide modified trisiloxane Wetting agent 5.00
Polyoxyethylene sorbitan monooleate Emulsifier 17.75
Ricinoleic acid ethoxylate Emulsifier 17.25
Calcium salt of alkylbenzene sulfonate Emulsifier 1.08
Isobutanol Solvent/co-solvent 7.10
Tetrahydrofurfuryl alcohol Solvent/co-solvent 23.06
Total 100.00


Storage Stability: Compound A 2.67% + Compound B 20.83% + Compound C 5.26% ME (Micro Emulsion)
Laboratory storage stability for 14 days
Parameters Specification Initial At 54±2 0C At 0±2 0C
Compound A content ( %, w/w) 2.38 to 2.75 2.62 2.61 2.62
Compound B content (%, w/w) 19.00 to 21.00 20.18 20.17 20.18
Compound C content (%, w/w) 4.74 to 5.50 5.10 5.09 5.10
pH range (1% aq. Suspension) 5.5 to 8.0 5.80 5.77 5.80
Emulsion stability Stable Stable Stable Stable
Specific gravity 1.01-1.05 1.02 1.02 1.02
Persistent foam ml (after 1 minute) max. 60 20 22 20


Room temperature storage stability up to 12 months
Parameters Specification 1 month 6 month 12 month
Compound A content ( %, w/w) 2.38 to 2.75 2.62 2.61 2.60
Compound B content (%, w/w) 19.00 to 21.00 20.18 20.18 20.16
Compound C content (%, w/w) 4.74 to 5.50 5.10 5.09 5.07
pH range (1% aq. Suspension) 5.5 to 8.0 5.80 5.78 5.77
Emulsion stability Stable Stable Stable Stable
Specific gravity 1.01-1.05 1.02 1.02 1.02
Persistent foam in ml (after 1 minute) max. 60 20 20 20
The composition of Compound A 2.67% + Compound B 20.83% + Compound C 5.26% ME (Micro Emulsion) meets the all inhouse specifications for storage stability studies in laboratory (at 54±2 °C and at 0±2 °C for 14 days) and room temperature (for 12 months).


Manufacturing process for 100 kg batch of Compound A 2.67% + Compound B 20.83% + Compound C 5.26% ME (Micro Emulsion)
Step 1 The solvent tetrahydrofurfuryl alcohol (23.06 kg) and isobutanol (7.10 kg) were charged into the vessel. Further, Compound A technical (2.67 kg), Compound B technical (20.83 kg) and Compound C technical (5.26 kg) were slowly added and mixed thoroughly till it gets completely dissolved.
Step 2 Polyoxyethylene sorbitan monooleate (17.75 kg), ricinoleic acid ethoxylate (17.25 kg) and calcium salt of alkylbenzene sulfonate (1.08 kg) were added into this premix slowly and homogenised to achieve uniform solution.
Step 3 Polyalkyleneoxide modified trisiloxane (5 kg) was added and mixed thoroughly to achieve uniform clear solution and then sent to QC for quality check.


EC formulation Range Actual
Description content (% w/w) -
Compound A technical 2 to 10 -
Compound B technical 15 to 35 -
Compound C technical 3 to 10 -
Wetting agent 2 to 10 5.00
Emulsifier 10 to 30 12.00
Solvent/Co-solvent 20 to 40 33.94


Composition of Compound A 4.10% + Compound B 31.25% + Compound C 7.90% EC (Emulsifiable Concentrate)
Ingredients Description content (% w/w)
Compound A technical (93.5% purity) Active Ingredient 4.10
Compound B technical (96% purity) Active Ingredient 31.25
Compound C technical (95% purity) Active Ingredient 7.90
Polyalkyleneoxide modified trisiloxane Wetting agent 5.00
Tristyrylphenol ethoxylate Emulsifier 6.75
Calcium salt of alkylbenzene sulfonate Emulsifier 3.00
Vegetable oil ethoxylates Emulsifier 2.25
Gamma butyrolactone Solvent/Co-solvent 5.81
Propylene carbonate Solvent/Co-solvent 3.75
Solvent naphtha Solvent/Co-solvent 30.19
Total 100.00


Storage Stability: Compound A 4.10% + Compound B 31.25% + Compound C 7.90% EC (Emulsifiable Concentrate)
Laboratory storage stability for 14 days
Parameters Specification Initial at 54±2 °C at 0±2 °C
Compound A content ( %, w/w) 3.56 to 4.13 3.79 3.78 3.79
Compound B content (%, w/w) 28.50 to 31.50 30.19 30.18 30.19
Compound C content (%, w/w) 7.13 to 8.25 7.58 7.56 7.58
pH range (1% aq. Emulsion) 5.5 to 8.0 5.80 5.84 5.80
Emulsion stability Stable Stable Stable Stable
Specific gravity 0.9-1.04 1.01 1.01 1.01
Persistent foam ml (after 1 minute) max. 60 10 12 10


Room temperature storage stability up to 12 months
Parameters Specification 1 month 6 month 12 month
Compound A content ( %, w/w) 3.56 to 4.13 3.79 3.78 3.78
Compound B content (%, w/w) 28.50 to 31.50 30.19 30.19 30.18
Compound C content (%, w/w) 7.13 to 8.25 7.58 7.57 7.56
pH range (1% aq. Suspension) 5.5 to 8.0 5.80 5.81 5.82
Emulsion stability Stable Stable Stable Stable
Specific gravity 0.9-1.04 1.01 1.01 1.01
Persistent foam in ml (after 1 minute) max. 60 10 12 12
The composition of Compound A 4.10% + Compound B 31.25% + Compound C 7.90% EC (Emulsifiable Concentrate) meets the all inhouse specifications for storage stability studies in laboratory (at 54±2 °C and at 0±2 °C for 14 days) and room temperature (for 12 months).


Manufacturing process for 100 kg batch of Compound A 4.10% + Compound B 31.25% + Compound C 7.90% EC (Emulsifiable Concentrate)
Step 1 The solvent naphtha (30.19 kg), gamma butyrolactone (5.81 kg) and propylene carbonate (3.75 kg) were charged into the vessel and then Compound A technical (4.10 kg), Compound B technical (31.25 kg) and Compound C technical (7.90 kg) were slowly added and mixed thoroughly till it gets completely dissolved.
Step 2 Tristyrylphenol ethoxylate (6.75 kg), Vegetable oil ethoxylate (2.25 kg) and Calcium salt of alkylbenzene sulfonate (3 kg) were added into this premix slowly and homogenised to achieve uniform solution.
Step 3 Polyalkyleneoxide modified trisiloxane (5 kg) was added and mixed thoroughly to achieve uniform clear solution and then sent to QC for quality check.

Overall field trials summary:
The present compositions of Compound A + Compound B+ Compound C shows synergism in terms of insect-pests control and produces more fruits, flowers, and grains, increases spectrum of control, reduces number of pesticidal applications under field conditions.

The process for preparing the insecticidal composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However, all such variation and modification is still covered by the scope of present invention.
Application to the seeds is carried out before sowing, either directly on the seeds or after having pregerminated the latter. Suitable application methods include inter alia soil treatment, seed treatment, in furrow application, and foliar application. Soil treatment methods include drenching the soil, drip irrigation (drip application onto the soil), dipping roots, tubers or bulbs, or soil injection. Seed treatment techniques include seed dressing, seed coating, seed dusting, seed soaking, and seed pelleting. In furrow applications typically include the steps of making a furrow in cultivated land, seeding the furrow with seeds, applying the pesticidally active composition to the furrow, and closing the furrow. Foliar application refers to the application of the pesticidally active composition to plant foliage, e.g. through spray equipment.
The rates of application vary within wide limits and depend on the nature of the soil, the method of application, the crop plant, the pest to be controlled, the prevailing climatic conditions, and other factors governed by the method of application, the time of application and the target crop.

The present invention is suitable for use in protecting crops, plants, plant propagation materials, such as seeds, or sailor water, in which the plants are growing, from attack or infestation by animal pests. Therefore, the present invention also relates to a plant protection method, which comprises contacting crops, plants, plant propagation materials, such as seeds, or soil or water, in which the plants are growing, to be protected from attack or infestation by animal pests, with an insecticidally effective amount of the present invention.
The present invention is also suitable for use in combating or controlling animal pests. Therefore, the present invention also relates to a method of combating or controlling animal pests, which comprises contacting the animal pests, their habitat, breeding ground, or food supply, or the crops, plants, plant propagation materials, such as seeds, or soil, or the area, material or environment in which the animal pests grow, with an insecticidally effective amount of the present invention.
The lists of crops on which the present invention is used include, but not limited to GMO (Genetically Modified Organism) and Non GMO traits, hybrids and conventional varieties of Cotton (Gossypium spp.), Paddy (Oryza sativa), Wheat (Triticum aestavum), Barley (Hordeum vulgare), Maize (Zea mays), Sorghum (Sorghum bicolor), Oat (Avena sativa), Pearl millet (Pennisetum glaucum), Sugarcane (Saccharum officinarum), Sugarbeet (Beta vulgaris), Soybean (Glycin max), Groundnut/Peanut (Arachis hypogaea), Sunflower (Helianthus annuus), Mustard (Brassica juncea), Rape seed (Brassica napus), Sesame (Sesamum indicum), Green gram (Vigna radiata), Black gram (Vigna mungo), Chickpea (Cicer aritinum), Cowpea (Vigna unguiculata), Red gram (Cajanus cajan), French bean (Phaseolus vulgaris), Indian bean (Lablab purpureus), Horse gram (Macrotyloma uniflorum), Field pea (Pisum sativum), Cluster bean (Cyamopsis tetragonoloba), Lentils (Lens culinaris), Brinjal (Solanum melongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschus esculentus), Onion (Allium cepa L.), Tomato (Solanum lycopersicun), Potato (Solanum tuberosum), Sweet potato (Ipomoea batatas), Chilly (Capsicum annum), Bell pepper (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumis sativus), Muskmelons (Cucumis melo), Watermelon (Citrullus lanatus), Bottle gourd (Lagenaria siceraria), Bitter gourd (Momordica charantia), Radish (Raphanus sativus), Carrot (Dacus carota subsp. sativus), Turnip (Brassica rapa rapa), Apple (Melus domestica), Banana (Musa spp.), Citrus groups (Citrus spp.), Grape (Vitis vinifera), Guava (Psidium guajava), Mango (Mangifera indica), Papaya (Carica papaya), Pineapple (Ananas comosus), Pomegranate (Punica granatum), Sapota (Manilkara zapota), Tea (Camellia sinensis), Coffea (Coffea Arabica), Turmeric (Curcuma longa), Ginger (Zingiber officinale), Cumin (Cuminum cyminum), Black Pepper (Piper nigrum), Mentha ( Mentha spp.), Rose (Rosa spp.), Jasmine (Jasminum spp.), Marigold ( Tagetes spp.), Common daisy (Bellis perennis), Dahlia (Dahlia hortnesis), Gerbera ( Gerbera jamesonii), Carnation (Dianthus caryophyllus).

Crops are to be understood as also including those crops which have been rendered tolerant to herbicides or classes of herbicides (e.g. ALS-, GS-, EPSPS-, PPO-, ACCase- and HPPD-inhibitors) by conventional methods of breeding or by genetic engineering. An example of a crop that has been rendered tolerant to imidazolinones, e.g. imazamox, by conventional methods of breeding is Clearfield® summer rape (canola). Examples of crops that have been rendered tolerant to herbicides by genetic engineering methods include e.g. glyphosate- and glufosinate-resistant maize varieties commercially available under the trade names RoundupReady® and LibertyLink®.

Crops are also to be understood as being those which have been rendered resistant to harmful insects by genetic engineering methods, for example Bt maize (resistant to European corn borer), Bt cotton (resistant to cotton boll weevil) and also Bt potatoes (resistant to Colorado beetle). Examples of Bt maize are the Bt 176 maize hybrids of NK® (Syngenta Seeds). The Bt toxin is a protein that is formed naturally by Bacillus thuringiensis soil bacteria. Examples of toxins, or transgenic plants able to synthesise such toxins, are described in EP-A-451 878, EP-A-374 753, WO 93/07278, WO 95/34656, WO 03/052073 and EP-A-427 529. Examples of transgenic plants comprising one or more genes that code for an insecticidal resistance and express one or more toxins are KnockOut® (maize), Yield Gard® (maize), NuCOTIN33B® (cotton), Bollgard® (cotton), NewLeaf® (potatoes), NatureGard® and Protexcta®. Plant crops or seed material thereof can be both resistant to herbicides and, at the same time, resistant to insect feeding ("stacked" transgenic events). For example, seed can have the ability to express an insecticidal Cry3 protein while at the same time being tolerant to glyphosate.

Crops are also to be understood to include those which are obtained by conventional methods of breeding or genetic engineering and contain so-called output traits (e.g. improved storage stability, higher nutritional value and improved flavour).

Other useful plants include turf grass for example in golf-courses, lawns, parks and roadsides, or grown commercially for sod, and ornamental plants such as flowers or bushes.

The insecticidal composition of present invention is used to control the following insects-pests.
The major insects pests belongs to the order Hemiptera, for example, rice leafhopper/green leaf hopper (GLH) Nephotettix nigropictus, rice brown plant hopper (BPH) Nilaparvata lugen, rice backed plant hopper (WBPH) Sogatella furcifera, Apple Mealy bug Phenococcus aceris, bean aphid Aphis fabae, black citrus aphid Toxoptera aurantii, citrus black scale Saissetia oleae, cabbage aphid Brevicoryne brassicae, Lipaphis erysimi, citrus red scale Aonidiella aurantii, yellow scale Aonidiella citrine, citrus mealybug Planococcus citri, corn leaf aphid Rhopalosiphum maidis, aphid Aphis gossypii, jassid Amrasca biguttula biguttla, mealy bug Planococcus spp. And Pseudococcus spp., cotton stainer Dysdercus suturellus, whitefly Bemisia tabaci, cowpea aphid Aphis crassivora, grain aphid Sitobion avenae, golden glow aphid Uroleucon spp., grape mealybug Pseudococcus maritimus, green peach aphid Myzus persicae, greenhouse whitefly Trialeurodes vaporariorum, papaya mealy bug Pracoccus marginatus, pea aphid Acyrthosiphon pisum, sugarcane mealybug Saccharicoccus sacchari, potato aphid Myzus persicae, potato leaf hopper Empoasca fabae, cotton whitefly Bemisia tabaci, tarnished plant bug Lygus lineolaris, wooly apple aphid Eriosoma lanigerum, mango hopper Amritodus atkinsoni, Idioscopus spp.; order Lepidoptera, army worm Mythimna unipuncta, asiatic rice borer Chilo suppressalis, beet armyworm Spodoptera exigua, fall armyworm Spodoptera frugiperda, black cutworm Agrotis ipsilon, bollworm Helicoverpa armigera, cabbage looper Trichoplusia ni, codling moth Cydia pomonella, croton caterpillar Achea janata, diamond backmoth Plutella xylostella, cabbage worm Pieris rapae, pink bollworm Pectinophora gossypiella, sugarcane borer Diatraea saccharalis, sugarcane early shoot borer Chilo infuscatellus tobacco budworm Heliothis virescens, tomato fruitworm Helicoverpa zea, velvet bean caterpillar Anticarsia gemmatalis, yellow stem borer Scirpophaga incertulas, spotted bollworm Earias vittella, rice leaffolder Cnaphalocrocis medinalis, pink stem borer Sesamia spp., tobacco leafeating caterpillar Spodoptera litura; brinjal fruit and shoot borer Leucinodes orbonalis, bean pod borer Maruca vitrata, Maruca testulalis, armyworm Mythimna separata, cotton pinkbollworm Pectinophora gossypiella, citrus leafminer Phyllocnistis citrella, cabbage butterfly Pieris bras-sicae, diamond backmoth Plutella xylostella, paddy stem borer Scirpophaga excerptallis, Scirpophaga incertulas, Scirpophaga innotata, wheat stem borer Sesamia inferens, Sitotroga cerealella, Spilosoma obliqua, fall armyworm Spodoptera frugiperda, Spodoptera littoralis, Spodoptera litura, Trichoplusia ni, Tryporyza nivella, Tryporyza incertulas, Tuta absoluta.

Further, from the order Coleoptera, for example, apple twig borer Amphicerus spp., corn root worm Diabrotica virgifera, cucumber beetle diabrotica balteata, boll weevil Anthonomus grandis, grape flea beetle Altica chalybea, grape root worm Fidia viticola, grape trunk borer Clytoleptus albofasciatus, radish flea beetle Phyllotreta armoraciae, maize weevil Sitophilus zeamais, northern corn rootworm Diabrotica barberi, rice water weevil Lissorhoptrus oryzophilus, Anthonomus grandis, Bruchus lentis, Diabrotica semipunctata, Diabrotica virgifera, Dicladispa armigera, Epilachna beetle, white grubs; from the order Orthoptera, for example, Gryllotalpa spp., Locusta spp., and Schistocerca is spp.; from the order Thysanoptera, for example, Thrips- Frankliniella spp., Thrips palmi, Thrips tabaci, Thrips parvispinus, Scirtothrips dorsalis; termites (Isoptera), e.g. Calotermes flavicollis, Coptotermes formosanus, Heterotermes aureus, Leucotermes flavipes, Microtermes obesi, Odontotermes obesus, Reticulitermes flavipes, Termes natalensis; from the order Heteroptera, for example, Dysdercus spp., Leptocorisa spp., from the order Hymenoptera, for example, Solenopsis spp. ; from the order Diptera, for example, Antherigona soccata, Dacus spp., Liriomyza spp., Melanagromyza spp., from the order Acarina, for example, Aceria mangiferae, Brevipalpus spp., Eriophyes spp., Oligonychus mangiferus, Oligonychus punicae, Panonychus citri, Panonychus ulmi, Polyphagotarsonemus latus, Tarsonemus spp., Tetranychus urticae, Tetranychus cinnabarinus.

The mixtures according to the present invention can be applied to any and all developmental stages of pests, such as egg, larva, pupa, and adult. The pests may be controlled by contacting the target pest, its food supply, habitat, breeding ground or its locus with a pesticidally effective amount of the inventive mixtures or of compositions comprising the mixtures.

The present invention has been described with reference to specific embodiment which is merely illustrative and not intended to limit the scope of the invention as defined in the present complete specification.
, Claims:We claim:
1. An insecticidal composition comprising:
A) Compound A: 4-((5RS)-5-(3,5-dichlorophenyl) -4,5-dihydro-5-(trifluoromethyl)isoxazol-3-yl) -N-((EZ)-(methoxyimino)methyl)-o-toluamide in an amount of 2 to 10 w/w%;
B) Compound B: 1-tert-butyl-3-(2,6-diisopropyl-4-phenoxyphenyl)thiourea in an amount of 15 to 35 w/w%; and
C) Compound C: 2-[1-(4-phenoxyphenoxy) propan-2-yloxy] pyridine in an amount of 4 to 10 w/w% and agrochemically acceptable excipients.

2. The insecticidal composition as claimed in claim 1, wherein the agrochemically acceptable excipients are selected from the group consisting of emulsifier(s), wetting agent(s), dispersing agent(s), suspending agent(s), anti freezing agent(s), antifoaming agent(s), thickener(s), rheology modifier(s)/stabilizer(s), solvent(s)/co-solvent(s) and preservative(s).

3. The insecticidal composition as claimed in claim 1, wherein the composition is in the form of dustable powder (DP), water soluble powder (SP), wettable powder (WP), wettable powder packed in water soluble pouch, water dispersible granules (WDG/WG), water soluble granule (SG), emulsifiable granule (EG), tablets (water soluble/dispersible), oil dispersible powder (OP), capsule suspension (CS), dispersible concentrate (DC), emulsifiable concentrate (EC), emulsion water-in-oil (EO), emulsion oil-in-water (EW), micro-emulsion (ME), oil-dispersion (OD), oil miscible flowable concentrate (OF), oil miscible liquid (OL), suspension concentrate (SC), suspo-emulsion (SE), soluble concentrate (SL), spreading oil (SO), ultra-low volume (ULV) suspension, ultra-low volume (ULV) liquid, zeon concentrate (ZC) mixed formulation of CS and SC, zoldine (ZE) mixed formulation of CS and SE, (ZW) mixed formulation of CS and EW.

4. The insecticidal composition as claimed in claim 3, wherein the preferable composition is in the form of suspension concentrate (SC), suspo emulsion (SE), oil dispersion (OD), emulsifiable concentrate (EC) and micro emulsion (ME).

5. The insecticidal composition as claimed in claim 2, wherein the emulsifier(s) is selected from polyarylphenyl anionic ether sulfate, ammonium salt, octylphenol ethoxylate, ethoxylated fatty acid, salt of dodecylbenzene sulphonate, Ca-salts, amine salt, sulphonate of C11-C16 alkylbenzene, calcium salt of alkylbenzene sulfonate, alkylether sulphate, alkylphenoletherphosphate, ester phosphate, non-ionic surfactant, alkoxylated alcohol, alkylphenol, ethoxylated fatty acid, ethoxylated vegetable oil, ethoxylated castor oil, fatty acid ester, sorbitol their ethoxylated derivative, ethoxylated amine, condensate of glycerol, catanionic emulsifier, cationic amine, alkylsulphonate, ether sulphonate, ether phosphate, alkoxylated alcohol, alkoxylated alkylphenol, ethoxylated vegetable oil, ethoxylated tristyrylphenol, tristyrlphenol with 16 moles EO, tristyrylphenol-polyglycolether-phosphate, fatty acid ester of sorbitol and ethoxylated derivative, ethoxylated amine and condensate of glycerol, sulfonated alkylbenzene in C11-C16 and salt, alkylether sulphate, alkyletherphosphate, alkylphenoletherphosphate, salt of phosphate ester of ethoxylated tristyrylphenol, salt of sulphated ether of ethoxylated tristyrylphenol, catanionic system, alkylsulphonate, alkylethersulphonate, ether sulphate, ether phosphate, alkyletherphosphate, nonylphenol polyethoxy ethanol, castor oil polyglycol ether, polyadduct of ethylene oxide and polypropylene, tributyl phenoxy polyethoxy ethanol, octyl phenoxy polyethoxy ethanol, polyarylphenyl anionic ether sulfate, ammonium salt, octylphenol ethoxylate, polyoxyethylene sorbitan monooleate, ricinoleic acid ethoxylate and mixture thereof.

6. The insecticidal composition as claimed in claim 2, wherein the wetting agent(s) is selected from polyalkyleneoxide modified trisiloxane, ethylene oxide/propylene oxide (EO/PO) block copolymer, poly aryl phenyl ether phosphate, polyalkoxylated butyl ether, ethoxylated fatty alcohol, sodium dioctyl sulfosuccinate, sodium lauryl sulfate, sodium dodecyl benzene sulfonate, alkyl diphenyl sulfonate, sodium isopropyl naphthalene sulfonate, alkyl naphthalene sulfonate, ethoxylated alcohol, natural fatty alcohol, lauryl alcohol ethoxylate, lauryl alcohol alkoxylate, synthetic alcohol ethoxylate, tridecyl alcohol ethoxylate, 2-ethyl hexanol, 2-propylheptanol, isodecyl alcohol, trisiloxane ethoxylate, heptamethyl trisiloxane, modified form of polyalkyleneoxide modified heptamethyl trisiloxane, polyether modified polysiloxane, polyalkyleneoxide modified trisiloxane, polyalkyleneoxide modified polydimethylsiloxane and mixture thereof.

7. The insecticidal composition as claimed in claim 2, wherein the dispersing agent(s) is selected from ethoxylated tristrylphenol, butyl polyalkylene oxide block copolymer, ethylene-propylene oxide block copolymer, tristyrylphenol-polyglycolether-phosphate, sodium naphthalene sulphonate formaldehyde condensate, alkylated naphthalene sulfonate, sodium salt, sodium salt of naphthalene sulfonate condensate, sodium ligno sulfonate, sodium polycarboxylate, phenol sulfonate, sodium methyl oleoyl taurate, styrene acrylic acid copolymer, propylene oxide-ethylene oxide-copolymer, polyethylene glycol 2,4,6-tristyrylphenyl ether, tristyrylphenole with 16 moles EO, oleyl-polyglycol ether with ethylene oxide, tallow fatty amine polyethylene oxide, nonylphenol polyglycol ether with 9-10 moles ethylene oxide, dodecyl benzene sulfonate in methylated, ethoxylated seed oil and mixture thereof.

8. The insecticidal composition as claimed in claim 2, wherein the suspending agent(s) is selected from aluminium magnesium silicate, bentonite clay, silica, attapulgite clay and mixture thereof.

9. The insecticidal composition as claimed in claim 2, wherein the anti freezing agent(s) is selected from 1,2-propylene glycol, ethylene glycol, propane diol, glycerine, urea, glycol, monoethylene glycol, diethylene glycol, polypropylene glycol, polyethylene glycol, magnesium sulfate heptahydrate, sodium chloride and mixture thereof.

10. The insecticidal composition as claimed in claim 2, wherein the anti foaming agent(s) is selected from polydimethylsiloxane, silicone antifoam emulsion, dimethyl siloxane, vegetable oil based antifoam, tallow based fatty acid and mixture thereof.

11. The insecticidal composition as claimed in claim 2, wherein the thickener(s) is selected from xanthan gum, polyvinylcarbazole (PVK), carboxymethyl cellulose, polyvinyl alcohol, gelatin, sodium carboxymethylcellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, acacia gum and mixture thereof.

12. The insecticidal composition as claimed in claim 2, wherein the rheology modifier(s)/stabilizer(s) is selected from precipitated silica, clay and mixture thereof.

13. The insecticidal composition as claimed in claim 2, wherein the solvent(s)/co-solvent(s) is selected from diluent water, water, water soluble alcohol, methyl ester of oleic acid, dihydroxy alcohol ether, lower alcohol, 1-4 carbon atom, methanol, ethanol, n-propanol, isopropanol, n-butanol, tert-butanol, macromolecular alcohol, polyethylene glycol, sorbitol, glucitol, dihydroxy alcohol ether, dihydroxy alcohol alkyl ether, dihydroxy alcohol aryl ether, ethylene glycol methyl ether, diethylene glycol methyl ether, propylene glycol methyl ether, di-propylene glycol methyl ether, ethylene glycol ethyl ether, diethylene glycol ethyl ether, propylene glycol ethyl ether, di-propylene glycol ethyl ether, aryl ether, ethylene glycol phenyl ether, 5 diethylene glycol phenyl ether, propylene glycol phenyl ether, di-propylene glycol phenyl ether, hydrocarbon, n-pentane, hexane, cyclohexane, methylcyclohexane, heptane, isooctane, benzene, toluene, xylene, isophorone, ester solvent, methyloleate, dimethylamide, morpholineamide derivative of C6-C16 fatty acid, mono-alkylene carbonate, ethylene carbonate, propylene carbonate, butylene carbonate, dimethylsulfoxide (DMSO), 2-ethylhexanol, n-butanol, n-alkylpyrrolidone, fatty acid dimethyl ester, fatty acid ester, dibasic esters, aromatic hydrocarbon, aliphatic hydrocarbon, dimethylamide, C8-dimethylamide, C10-dimethylamide, C12-dimethylamide, ethylene glycol, propylene glycol, polyalkylene glycol, aromatic hydrocarbon, dimethylformamide (DMF), dimethylisosorbide (DMI), isophorone, acetophenone, 1,3-dimethyl-2-imidazolidonone, lactate ester, dimethyl, diethylcarbonate, alcohol, methanol, ethanol, iso-propanol, n-propanol, n-butanol, iso-butanol, tert-butanol, methyl L-lactate, 2-ethylhexyl L-lactate, ethyl L-lactate, n-butyl L-lactate, gamma butyrolactone, propylene carbonate, vegetable oil, methylated vegetable oil, ethylated vegetable oil, olive oil, kapok oil, castor oil, papaya oil, camellia oil, sesame oil, corn oil, rice bran oil, cotton seed oil, soybean oil, groundnut oil, rapeseed-mustard oil, linseed oil, tung oil, sunflower oil, safflower oil, coconut oil, alkyl ester of vegetable oils, methyl ester, ethyl ester, propyl ester, butyl ester of vegetable oil, methylated seed oil, polyalkyleneoxide modified polydimethylsiloxane alkylphenol ethoxylate, oleic acid methyl ester, rapeseed oil methyl ester, rapeseed oil ethyl ester, rapeseed oil propyl ester, rapeseed oil butyl ester, soybean oil methyl ester, soybean oil ethyl ester, soybean oil propyl ester, soybean oil butyl ester, castor oil methyl ester, castor oil ethyl ester, castor oil propyl ester, castor oil butyl ester, cotton seed oil methyl ester, cotton seed oil ethyl ester, cotton seed oil butyl ester, cotton seed oil propyl ester, tall oil fatty acid esters-tallow methyl ester, tallow ethyl ester, tallow propyl ester, bio-diesel, mineral oil, aromatic solvent, isoparaffin, base solvent, fatty acid amide, C1-C3 amine, alkylamine, alkanolamine with C6-C18 carboxylic acid, fatty acid, alkyl ester of fatty acid, methyl oleate, ethyl oleate, methyl soyate, ethyl soyate, alkyl benzene, alkylnaphthalene, polyalkylene glycol ether, fatty acid diester, fatty alkylamide, fatty diamide, dialkylene carbonate, ketone, alcohol, cyclohexanone, acetophenone, N-methyl-2-pyrrolidinone (NMP), dimethyl sulfoxide, benzyl alcohol, butanol, N-octanol, N-propanol, 2-ethyl hexanol, tetrahydro furfuryl alcohol, isophorone, fatty acid dimethyl amide, 2-hexylethyl lactate, propylene carbonate and mixture thereof.

14. The insecticidal composition as claimed in claim 2, wherein the preservative(s) is selected from 1,2-benzisothiazolin-3(2H)-one, sodium salt, sodium benzoate, 2-bromo-2-nitropropane-1,3-diol, formaldehyde, sodium o-phenyl phenate, 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one and mixture thereof.

Dated this on 4th November, 2024

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