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A PROCESS OF DETECTION OF ORGANOPHOSPHATE PESTICIDE BASED ON FLUORESCENCE SPECTROSCOPY

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A PROCESS OF DETECTION OF ORGANOPHOSPHATE PESTICIDE BASED ON FLUORESCENCE SPECTROSCOPY

ORDINARY APPLICATION

Published

date

Filed on 22 November 2024

Abstract

TITLE: A PROCESS OF DETECTION OF ORGANOPHOSPHATE PESTICIDE BASED ON FLUORESCENCE SPECTROSCOPY APPLICANT: MEENAKSHI ACADEMY OF HIGHER EDUCATION AND RESEARCH ABSTRACT The present invention discloses a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy. The process of the present invention comprises of following steps; a. preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder and treating the fine powder with HCl and adding NaOH followed by adding ammonia and polyethylene glycol and transferring to hydrothermal reactor and heating followed by centrifuging using ethanol and dialyzing against double distilled water to obtain purified carbon quantum dots; b. mixing purified carbon quantum dots with ethylenediamide and adding to sample containing organophosphate pesticide and allowing to stand to equilibrate and subjecting to fluorescence spectroscopy in which decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.

Patent Information

Application ID202441090786
Invention FieldPHYSICS
Date of Application22/11/2024
Publication Number48/2024

Inventors

NameAddressCountryNationality
MR. BHARATH PALANIRESEARCH SCHOLAR, DEPARTMENT OF RESEARCH, MEENAKSHI ACADEMY OF HIGHER EDUCATION AND RESEARCH, NO.12, VEMBULIAMMAN KOIL STREET, WEST K.K. NAGAR CHENNAI CHENNAI TAMIL NADU INDIA 600078IndiaIndia
DR. THIRUMAL KUMAR DASSISTANT PROFESSOR & ASSISTANT RESEARCH DIRECTOR, FACULTY OF ALLIED HEALTH SCIENCES, MEENAKSHI ACADEMY OF HIGHER EDUCATION AND RESEARCH, NO.12, VEMBULIAMMAN KOIL STREET, WEST K.K. NAGAR CHENNAI CHENNAI TAMIL NADU INDIA 600078IndiaIndia

Applicants

NameAddressCountryNationality
MEENAKSHI ACADEMY OF HIGHER EDUCATION AND RESEARCHNO.12, VEMBULIAMMAN KOIL STREET WEST K.K. NAGAR CHENNAI CHENNAI TAMIL NADU INDIA 600 078IndiaIndia

Specification

Description:Form 2


THE PATENT ACT, 1970
(39 of 1970)
&
THE PATENT RULES, 2003
COMPLETE SPECIFICATION
(See section 10 and rule 13)





"A PROCESS OF DETECTION OF ORGANOPHOSPHATE PESTICIDE BASED ON FLUORESCENCE SPECTROSCOPY"






in the name of MEENAKSHI ACADEMY OF HIGHER EDUCATION AND RESEARCH an Indian National having address at NO.12, VEMBULIAMMAN KOIL STREET, WEST K.K. NAGAR, CHENNAI, CHENNAI - 600 078, TAMIL NADU, INDIA.,


The following specification particularly describes the invention and the manner in which it is to be performed.


SOURCE AND GEOGRAPHICAL ORIGIN OF THE BIOLOGICAL MATERIAL:

SL.
NO COMMON NAME SCIENTIFIC NAME PART OF BIOLOGICAL SOURCES SOURCE OF ACCESS DETAILS OF GEOGRAPHICAL LOCATION
1. Pomegranate Punica granatum Fruit-Peel Market (i) Name of the Market:
Kovai Pazhamudir Nilayam.

(ii) Contact details:
No.5/3 Plot,
Senthil Andavar Fruits,
66, Ramaswamy Salai, Sector 11,
K. K. Nagar,
Chennai - 600078,
Tamil Nadu,
India.

FIELD OF THE INVENTION:

The present invention generally relates to the field of agriculture. Specifically, the present invention relates to detection of organophosphate pesticide. More particularly, the present invention relates to a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy.

BACKGROUND OF THE INVENTION:

Organophosphorus pesticides are widely used as insecticides in agricultural production because of their wide range of control targets and low cost. A large amount of organic phosphorus widely exists in food, water and soil, which poses great threat to human health and also seriously affects the ecological system and homeland safety. Research confirms that this kind of agricultural chemicals can produce toxic action to the nervous system of human body includes carcinogenic, teratogenesis, mutagenesis and certain accumulative, and excessive use is likely accumulated in human body, causes chronic accumulate poisoning.

Organophosphorus pesticides remaining on agricultural crops or fruit trees irreversibly inhibit the activity of acetylcholinesterase (AChE) in the human body, leading to accumulation of the neurotransmitter acetylcholine in the body, eventually leading to organ failure and death. Therefore, the detection of organophosphorus pesticides has become an urgent problem. At present, the organophosphorus pesticide detection and analysis technology includes HPLC, liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS) and the like.

There are reports available in the state of art relating to method of detection of organophosphorus pesticide.

CN101799460A discloses a method for detecting the organophosphorus pesticide residue in the tobacco, comprising the following steps of preprocessing by adding water of 5-15 times the weight of the tobacco sample to the tobacco sample to sock the tobacco sample, adding 10-20 ml of extracting agent to each gram of the tobacco sample to extract the tobacco sample, centrifuging to obtain organic-phase supernatant, purifying the organic-phase supernatant, concentrating the organic-phase supernatant by blowing with nitrogen, adding acetone solution to the organic-phase supernatant, and filtering the organic-phase supernatant with a organic-phase filtering membrane of 0.2 Mum to obtain the sample solution, wherein the extracting agent is the mixed solution of acetonitrile and acetone which are at the volume ratio of 1-2:1; and detecting the organophosphorus pesticide residue in the sample solution with gas chromatography and mass spectroscopy by using the chlorazol phosphorus as the internal standard substance after finishing preprocessing. Because the extracting agent is used for extracting in the preprocessing step, the target substance in the tobacco sample can be effectively extracted into the organic phase, and the precision for detecting the organophosphorus pesticide residue in the tobacco can be greatly improved.

CN103439436A discloses a gas chromatography method for detecting the residual quantity of eight organophosphorus pesticides contained in soil (loamy soil, clay and sandy soil) with different textures, and the gas chromatography method realizes the qualitative and quantitative detection of the pesticide residues of acephate, omethoate, dimethoate, phorate, malathion, fenitrothion, isocarbophos and hostathion. The gas chromatography method is characterized in that a dichloromethane-acetone (V:V=1:1) mixed solvent is used as an extracting agent; and an extracting solution is concentrated, metered in volume and filtered and then directly subjected to trace analysis on a gas chromatograph. The gas chromatography method disclosed by the invention has the advantages of good separation effect of chromatographic columns on target objects, high detection result accuracy and good repeatability and meets the detection requirements of residual pesticides on sensitivity; compared with the GB/T14552-2003 (gas chromatography method for detecting the organophosphorus pesticides contained in water and soil) national standard method, the gas chromatography method disclosed by the invention is simpler and more convenient, simplifies the preprocessing steps of a soil sample, shortens the detection time and saves the cost.

CN107490647B discloses a method for quantitatively detecting organophosphorus pesticide chlorpyrifos in ambient air, belonging to the field of pesticide residue detection methods. The invention utilizes the multi-reaction monitoring technology of high performance liquid chromatography/tandem mass spectrometry to overcome the defect that the selectivity and the sensitivity of the traditional detector for detecting the trace chlorpyrifos compound in the complex background matrix cannot be obtained at the same time, and the qualitative and the quantitative determination are more accurate and reliable, so the invention has very obvious advantages compared with the traditional UV-Vis, HPLC/PDA, GC/ECD and GC/NPD.

Though conventional method of detection of organophosphorus pesticide exhibits advantages in the prior arts they do have disadvantages such as, complex process, expensive and time consumption.

Thus there exists a need in the state of art for developing an alternative method for detection of organophosphate pesticide.

Hence an attempt has been made to develop a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy overcoming the above said drawbacks.

OBJECT OF THE INVENTION:

The main object of the present invention is to develop a process of detection of organophosphate pesticide.

Another object of the present invention is to develop a process of detection of organophosphate pesticide employing carbon quantum dots from Pomegranate peels and ethylenediamide.

Yet another object of the present invention is to develop a sustainable and cost effective method for detection of organophosphate pesticide.
Further object of the present invention is to utilize the method for detection of organophosphate pesticide from sample through fluorescence spectroscopy.

BRIEF DESCRIPTION OF DRAWINGS:
Figure 1 depicts the process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy of the present invention.

SUMMARY OF THE INVENTION:

The present invention discloses a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy. The process of the present invention comprises of following steps;

a. preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder and treating the fine powder with HCl and adding NaOH followed by adding ammonia and polyethylene glycol and transferring to hydrothermal reactor and heating followed by centrifuging using ethanol and dialyzing against double distilled water to obtain purified carbon quantum dots;
b. mixing purified carbon quantum dots with ethylenediamide and adding to sample containing organophosphate pesticide and allowing to stand to equilibrate and subjecting to fluorescence spectroscopy in which decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.


DETAILED DESCRIPTION OF THE INVENTION:

The present invention discloses a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy. The process of the present invention comprises of preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder. Then, treating the fine powder with HCl and adding NaOH followed by adding ammonia and polyethylene glycol. Then, transferring to hydrothermal reactor and heating followed by centrifuging using ethanol and dialyzing against double distilled water to obtain purified carbon quantum dots.

Mixing purified carbon quantum dots with ethylenediamide and adding to sample containing organophosphate pesticide and allowing to stand to equilibrate and subjecting to fluorescence spectroscopy in which decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.

The use of pomegranate peels as a carbon source may introduce a more standardized and reproducible synthesis approach and also addresses environmental concerns. Pomegranate peels are generally considered safe and edible thereby enhance their biocompatibility and reduce potential toxicity concerns.

Presence or absence of organophosphate pesticide in soil samples (100gm) were detected using the method of the present invention to ascertain its specificity and compared with HPLC method.




Table 1: Comparative study between process of the present invention and HPLC method.

Sample Results obtained employing detection process of the present invention Results obtained employing HPLC method

Soil sample Present Present
Absent Absent

Thus from the above, it was found that the process of the present invention be a suitable candidate for detecting organophosphate pesticide from sample based on fluorescence spectroscopy.

In one of the preferred embodiments, the present invention shall disclose a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy. The process of the present invention comprises of following steps;

a. preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder and treating the fine powder with HCl and adding NaOH followed by adding ammonia and polyethylene glycol and transferring to hydrothermal reactor and heating followed by centrifuging using ethanol and dialyzing against double distilled water to obtain purified carbon quantum dots;
b. mixing purified carbon quantum dots with ethylenediamide and adding to sample containing organophosphate pesticide and allowing to stand to equilibrate and subjecting to fluorescence spectroscopy in which decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.

In further preferred embodiment, the present invention shall disclose a process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy. The process of the present invention comprises of following steps;

a. preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder and treating 2g of the fine powder with 15mL of 3MHCl for 30mins and adding 10mL of 1M NaOH followed by adding 10mL of ammonia and 10mL of polyethylene glycol and transferring to hydrothermal reactor and heating to 200°C for a period of 12 hours followed by centrifuging using ethanol and dialyzing against double distilled water for 4 days at room temperature to obtain purified carbon quantum dots;
b. mixing1mL of purified carbon quantum dots with 1mL of ethylenediamide and adding to 1mL of sample containing organophosphate pesticide and allowing to stand for 30mins to equilibrate and subjecting to fluorescence spectroscopy in which decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.





Working example:

A process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy.

Washed Pomegranate peels were dried and grinded to form a fine powder. The obtained 2g of fine powder was treated with 15mL of 3M HCl for 30mins and also 10mL of 1M NaOH was added. Then 10mL of ammonia and 10mL of polyethylene glycol were added and transferred to hydrothermal reactor and heated to 200°C for a period of 12 hours. Then, centrifuged using ethanol and dialyzed against double distilled water for 4 days at room temperature to obtain purified carbon quantum dots. The obtained 1mL of purified carbon quantum dots was mixed with 1mL of ethylenediamide and was added to 1mL of sample containing organophosphate pesticide. Then, allowed to stand for 30mins to equilibrate and subjected to fluorescence spectroscopy in which decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.

Although the invention has now been described in terms of certain preferred embodiments and exemplified with respect thereto, one skilled in art can readily appreciate that various modifications, changes, omissions and substitutions may be made without departing from the scope of the following claims.
, Claims:WE CLAIM:

1. A process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy, comprises of following steps;
a. preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder and treating the said fine powder with HCl and adding NaOH followed by adding ammonia and polyethylene glycol and transferring to hydrothermal reactor and heating followed by centrifuging using ethanol and dialyzing against double distilled water to obtain purified carbon quantum dots;
b. mixing purified carbon quantum dots with ethylenediamide and adding to sample containing organophosphate pesticide and allowing to stand to equilibrate and subjecting to fluorescence spectroscopy wherein decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.

2. A process of detection of organophosphate pesticide from sample based on fluorescence spectroscopy, comprises of following steps;
a. preparation of carbon quantum dots by drying and grinding washed Pomegranate peels to form a fine powder and treating 2g of the said fine powder with 15mL of 3M HCl for 30mins and adding 10mL of 1M NaOH followed by adding 10mL of ammonia and 10mL of polyethylene glycol and transferring to hydrothermal reactor and heating to 200°C for a period of 12 hours followed by centrifuging using ethanol and dialyzing against double distilled water for 4 days at room temperature to obtain purified carbon quantum dots;
b. mixing 1mL of purified carbon quantum dots with 1mL of ethylenediamide and adding to 1mL of sample containing organophosphate pesticide and allowing to stand for 30mins to equilibrate and subjecting to fluorescence spectroscopy wherein decrease in fluorescence in comparison to control indicates presence of organophosphate pesticide else absence of organophosphate pesticide.


Dated this 21st day of NOV 2024


For MEENAKSHI ACADEMY OF HIGHER
EDUCATION AND RESEARCH
By its Patent Agent

Dr.B.Deepa
IN/PA 1477

Documents

NameDate
202441090786-COMPLETE SPECIFICATION [22-11-2024(online)].pdf22/11/2024
202441090786-DECLARATION OF INVENTORSHIP (FORM 5) [22-11-2024(online)].pdf22/11/2024
202441090786-DRAWINGS [22-11-2024(online)].pdf22/11/2024
202441090786-FIGURE OF ABSTRACT [22-11-2024(online)].pdf22/11/2024
202441090786-FORM 1 [22-11-2024(online)].pdf22/11/2024
202441090786-FORM 18 [22-11-2024(online)].pdf22/11/2024
202441090786-FORM-8 [22-11-2024(online)].pdf22/11/2024
202441090786-FORM-9 [22-11-2024(online)].pdf22/11/2024
202441090786-POWER OF AUTHORITY [22-11-2024(online)].pdf22/11/2024
202441090786-REQUEST FOR EARLY PUBLICATION(FORM-9) [22-11-2024(online)].pdf22/11/2024
202441090786-REQUEST FOR EXAMINATION (FORM-18) [22-11-2024(online)].pdf22/11/2024

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