A METHOD FOR PREPARING ANTI-DIABETIC THERAPEUTIC FORMULATION DERIVED FROM CANNABIS SATIVA AND ZIZANIA LATIFOLIA

Abstract

ABSTRACT A method (100) for preparing anti-diabetic therapeutic formulation derived from Cannabis sativa and Zizania latifolia. Further, the method (100) comprising extracting active compounds from Cannabis sativa and Zizania latifolia using a suitable solvent selected from the group consisting of ethanol, methanol, and aqueous solutions, at a temperature ranging from 25°C to 60°C, for a duration of 2 to 24 hours. Further, filtering the extract to remove solid residues and obtaining a clear liquid extract containing the active phytochemicals. Further, concentrating the filtered extract under reduced pressure at a temperature not exceeding 40°C to obtain a concentrated extract. Further, mixing the concentrated extract with pharmaceutically acceptable excipient. Further, characterizing the anti-diabetic therapeutic formulation for DPP4 and PPAR inhibitory activity using computational approach (i.e. Molecular docking) and in-silico pharmacokinetics.

Specification

Description:FIELD OF THE DISCLOSURE
[0001] This invention generally relates to a field of therapeutic formulation and, in particular to a method for preparing anti-diabetic therapeutic formulation derived from Cannabis sativa and Zizania latifolia.
BACKGROUND

[0002] The subject matter discussed in the background section should not be assumed to be prior art merely as a result of its mention in the background section. Similarly, a problem mentioned in the background section or associated with the subject matter of the background section should not be assumed to have been previously recognized in the prior art. The subject matter in the background section merely represents different approaches, which in and of themselves may also correspond to implementations of the claimed technology.
[0003] The growing interest in utilizing natural products for therapeutic purposes has surged in recent years, particularly in the context of managing chronic metabolic disorders such as diabetes. Cannabis sativa and Zizania latifolia, b , Claims:1. A method (100) for preparing anti-diabetic therapeutic formulation derived from Cannabis sativa and Zizania latifolia, the method comprising:
extracting active compounds from Cannabis sativa and Zizania latifolia using a suitable solvent selected from the group consisting of ethanol, methanol, and aqueous solutions, at a temperature ranging from 25°C to 60°C, for a duration of 2 to 24 hours, at step 102;
filtering the extract to remove solid residues and obtaining a clear liquid extract containing the active phytochemicals, at step 104;
concentrating the filtered extract under reduced pressure at a temperature not exceeding 40°C to obtain a concentrated extract, at step 106;
mixing the concentrated extract with pharmaceutically acceptable excipient, at step 108; and
characterizing the anti-diabetic therapeutic formulation for DPP4 and PPAR inhibitory activity using computational approach (i.e. Molecular docking) and in-silico pharmacokinetics, at step 110.

2. The method (100) as claimed in claim 1, wh

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